Báo cáo khoa học: GTP binding and hydrolysis kinetics of human septin 2 pot

... presence of 15 20 lm SEPT2 and 5 mm MgCl 2 and 100 lm of eachGTPase properties of human SEPT2 Y W. Huang et al. 325 8 FEBS Journal 27 3 (20 06) 324 8– 326 0 ª 20 06 The Authors Journal compilation ª 20 06 ... properties of human SEPT2 Y W. Huang et al. 326 0 FEBS Journal 27 3 (20 06) 324 8– 326 0 ª 20 06 The Authors Journal compilation ª 20 06 FEBS GTP binding and hydrolysis kinetics of human septin 2 Yi-Wei Huang1, ... exchange and GTP hydrolysis reactions of Rho family GTP- bind-ing proteins. J Biol Chem 27 5, 25 299 25 307.35 Zhao J, Wang WN, Tan YC, Zheng Y & Wang ZX (20 02) Effect of Mg (2+ ) on the kinetics of...

Báo cáo khoa học: Ligand binding and antigenic properties of a human neonatal Fc receptor with mutation of two unpaired cysteine residues docx

... alkylationC96-C159C48α1 2 C25-C80β2mC198-C2 52 C251α3AB 2. 0WT, pH 6.0WT, pH 7.4C48S/C251S, pH 6.0C48S/C251S, pH 7.41.51.00.50.00 25 50 75 100hlgG1 (nM)A405Fig. 1. Structure of shFcRn and pH-dependent ... C251(D), C251 ⁄ C2 52 (H) and C48 (I).FcRn HC mutant J. T. Andersen et al.41 02 FEBS Journal 27 5 (20 08) 4097–4110 ª 20 08 The Authors Journal compilation ª 20 08 FEBSin vitro refolding on hb2m. ... the development of IgGs with increased half-life [20 22 ] and improved imaging properties [23 25 ],as well as of Fc and albumin fusion products with anincreased half-life [26 29 ]. Furthermore,...

Báo cáo khoa học: DNA-binding and transcription characteristics of three cloned sigma factors from mustard (Sinapis alba L.) suggest overlapping and distinct roles in plastid gene expression doc

... (EC 2. 7.7.6).(Received 19 November 20 02, revised 23 January 20 03, accepted 3 February 20 03)Eur. J. Biochem. 27 0, 128 8–1300 (20 03) Ó FEBS 20 03 doi:10.1046/j.14 32- 1033 .20 03.03494.xIsolation of ... 1998),SASIG2 (AJ276656) and SASIG3 (AJ276657) from S. alba and r70from E. coli (U23083). The HTH motif in region 4 .2 is boxed. 129 0 A. Homann and G. Link (Eur. J. Biochem. 27 0) Ó FEBS 20 03possible ... eitheranti-SASIG2 (lanes 1 and 2) or anti-SASIG3 antisera (lanes 3 and 4).Ó FEBS 20 03 Recombinant plastid sigma factors (Eur. J. Biochem. 27 0) 129 1were capable of faithful and efﬁcient binding and transcrip-tion...

Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx

... 100Mg 2+ 1.0 100Mn 2+ 1.0 100Zn 2+ 0.1 6Ni 2+ 1.0 100Ca 2+ 1.0 100Fe3+0.1 15Fe 2+ 0.1 124 1.0 90Co 2+ 0.1 120 1.0 130Cr3+0.5 35Cd 2+ 0.5 25 Fig. 7. Lineweaver)Burk analysis of ACMSD ... acid 0.5 72 1.0 61Picolinic acid 0 .25 800.5 581.0 47 Human ACMSD L. Pucci et al.8 32 FEBS Journal 27 4 (20 07) 827 –840 ª 20 07 The Authors Journal compilation ª 20 07 FEBS(not shown) and, as expected, ... Takikawa O &Culler KM (20 05) Indoleamine 2, 3 dioxygenase and Human ACMSD L. Pucci et al.838 FEBS Journal 27 4 (20 07) 827 –840 ª 20 07 The Authors Journal compilation ª 20 07 FEBSquinolinic acid...

Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf

... 10 October 20 02, revised 8 January 20 03,accepted 5 February 20 03)Eur. J. Biochem. 27 0, 1 327 –1337 (20 03) Ó FEBS 20 03 doi:10.1046/j.14 32- 1033 .20 03.03503.xfound that the second isoform without ... recombinant Lyc e 2 isoforms rLyc e 2. 01 (lane 1) and rLyc e 2. 02 (lane 2) , Coomassie stain. M, molecular mass marker.13 32 S. Westphal et al. (Eur. J. Biochem. 27 0) Ó FEBS 20 03glycan analysis of the natural ... 3) 2/ 2 1 (n ¼ 1) 1/1 2( n ¼ 6) 1/1 2 (n ¼ 1) 0/13(n ¼ 5) 3/3 3 (n ¼ 5) 3/34(n ¼ 2) 2/ 2 4 (n ¼ 3) 3/35(n ¼ 1) 1/1 5 (n ¼ 0) 0/0Ó FEBS 20 03 Allergenic glycoprotein Lyc e 2 (Eur. J. Biochem. 27 0)...

Báo cáo khoa học: Puriﬁcation and functional characterization of human 11b hydroxylase expressed in Escherichia coli doc

... volume and energeticproperties of the binding of CO to hemoproteins.Biophys J 66, 89–98. 42 Tuckey RC & Kamin H (1983) Kinetics of O 2 and CO Binding to adrenal cytochrome P-450scc. Effect of ... thesignal at 326 can be attributed to an anisotropy of the porphyrin absorption band [22 ]. Upon addition of substrate, the signal of the positive CD bandat 29 0 nm and the negative band at 386 nm ... N & Bernhardt R (20 02) The effect of amino-acid substitutions I112P, D147E and K152Nin CYP11B2 on the catalytic activities of the enzyme.Eur J Biochem 26 9, 1118–1 127 .31 Nishihara K, Kanemori...

Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt

... Journal 27 2 (20 05) 1103–1116 ª 20 05 FEBS 1115cAMP-induced allosteric changes in T 127 I, S 128 A and T 127 I ⁄ S 128 A mutants of cAMP receptor protein fromEscherichia coli. J Biol Chem 27 8, 43 020 –43 026 .19 ... PolandFax: +48 12 66 46 9 02 Tel: +48 12 66 46 122 E-mail: wasylewski@mol.uj.edu.pl(Received 29 July 20 04, revised 22 November 20 04, accepted 21 December 20 04)doi:10.1111/j.17 42- 4658 .20 05.04540.xCyclic ... 1. 62 4.60 – 3. 92 CRPW85A–lac 4 .23 0.57 0. 62 2.68 2. 07 – 2. 29CRPW85A–gal 3.93 0.51 0. 62 2.31 2. 17 – 3.39Fig. 9. Kinetics of binding between IAEDANS-labeled CRP and ﬂuo-rescein-labeled DNA, as...

Báo cáo khoa học: Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B docx

... spectrum of XPCB–hHR23B.Fig. S2. Relaxation studies of XPCB–hHR23B at 600MHz ﬁeld.Dynamic structure of XPC -binding domain of hHR23B B. Kim et al. 24 76 FEBS Journal 27 2 (20 05) 24 67 24 76 ª 20 05 ... hHR23BFEBS Journal 27 2 (20 05) 24 67 24 76 ª 20 05 FEBS 24 71L298, L299, and P300) and thus form a U-shapedhydrophobic surface (Fig. 4A,B). The boundary and size of the hydrophobic linker patch and ... (A1), Kamionka and Feigon [20 ] (A2), and XPCB–hHR23B (B).Dynamic structure of XPC -binding domain of hHR23B B. Kim et al. 24 72 FEBS Journal 27 2 (20 05) 24 67 24 76 ª 20 05 FEBSsought to determine which...

Báo cáo khoa học: Oxygen binding and its allosteric control in hemoglobin of the primitive branchiopod crustacean Triops cancriformis pdf

... magnaPale 3.8 1.3 20 7 .2 Phosphate [71]Pale 8.3 20 WH [ 72] Pale 7.7 1.6a 25 7 .2 Bis-Tris ⁄ propane [57]Red 1.6 1.5 20 7 .2 Phosphate [71]Red 2. 5 20 WH [ 72] Red 2. 6 1.8a 25 7 .2 Bis-Tris ⁄ propane ... cancriformis 13 2. 3 20 7.4 Tris )0.18 )49 Present study14 2. 3 20 7.5 HepesLepidurus bilobatus 20 2 20 7 .2 Tris )0.13 )31 [66]Lepidurus lynchi 21 3 20 8.0 Tris )0.19 [67]Lepidurus couesi 22 3 20 8.0 ... s43 2 11/4 1 /2 1Number (m) of Mg 2+ -binding sites per hemeNumber (h) of proton -binding sites per hemeSSR 20 30 40 2 11 2 –Fig. 7. Gallery of error contour maps showing the dependence of SSR...

Báo cáo khoa học: Solution structure and internal dynamics of NSCP, a compact calcium-binding protein doc

... delays of 2. 5 s were used at thebeginning of R1 and R2 pulse sequences. Spectra for R1measurements were acquired using 11.04 (· 2) , 55 .22 ,165.66, 22 0.88 (· 2) , 386.54, 5 52. 2, 6 62. 64, 828 .3 and 1104.4 ... Thepositive and negative potential are conventionally coded in blue and red, respectively.Structure and dynamics of NSCP in solution G. Rabah et al. 20 24 FEBS Journal 27 2 (20 05) 20 22 20 36 ª 20 05 FEBStains ... Structure and dynamics of NSCP in solutionFEBS Journal 27 2 (20 05) 20 22 20 36 ª 20 05 FEBS 20 27Figure 4 shows the relaxation parameters (R1, R2,NOE) and their uncertainty plotted as a function of residue...

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