Báo cáo khoa học: cGMP transport by vesicles from human and mouse erythrocytes potx

Báo cáo khoa học: Linking pseudouridine synthases to growth, development and cell competition potx

... Cfb5p, rat NAP57, and mouse and human dyskerin [1]. These proteins localize in thenucleolus and are involved in a variety of essential cellu-lar functions, including processing and modiﬁcation ... support and helpful discussions, and to A. Mon-aco for contributing to Drosophila rearing. J. F. deCelis is supported by a grant (BFU2006-06501) from the Spanish Ministry of Education and Science.G. ... complexes, one involved in ribosomebiogenesis and snoRNA stability and the other in telo-mere maintenance. In humans, dyskerin is encoded by the DKC1 gene [15], and its loss of function is responsi-ble...

Tài liệu Báo cáo khoa học: The alr2505 (osiS) gene from Anabaena sp. strain PCC7120 encodes a cysteine desulfurase induced by oxidative stress docx

... production of pyruvate from cysteine by OsiS was measured and found to beapproximately 32 nmol pyruvateÆmin)1Æmg OsiS)1.Pyruvate can also be produced from cysteine by cyste-ine lyase enzymes ... to that of IscS(Fig. 1A), with a two-domain organization and thepresence of both a-helices and b-strands. On the basisof these data and the fact that alr2505-expression isspecifically induced ... approaches tested, solubilization by urea was found to be the most efficient method ofpurification, and this method was used to obtain theenzyme followed by removal of urea and refolding.Pure OsiS showed...

Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt

... described above are very similar from a regulatory point of view. dCMP deaminase is activa-ted by dCTP and inhibited by dTTP and both nucleo-tides act on the enzyme by binding to an allosteric siteto ... represent-ing free and total radioactive nucleotide were washed by TLC in 1 m acetic acid, cut out and quantiﬁed by liquidscintillation as above for samples from kinetic experiments.Data from presteady-state ... designated A and B,originating from the A and B chains in the structure.dTTP binds at the site of the protein shown previouslyto bind the nucleotides dUTP and dCTP in wild-type and the E138A...

Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx

... induced by ligands on C-LytA monitored by near-UV CD with no ligand added (—) and upon addition of ligands:s, d, 2.5 and 20 mM choline; n, m, 2.5 mM and 20 mM atropine;h, j), 2.5 mM and 20 ... min. Experimental con-ditions depended on the enzyme, and were set as follows:37 °C and pH 6.9 for LytA; 30 °C and pH 6.0 for LytC; and 30 °C and pH 6.9 for Pce. The speciﬁc activities of theenzymes ... different from thatof choline (Fig. 6B).To check whether the toxic effects of atropine and ipratropium might be reversed by addition of excessamounts of choline, most likely by their displacementfrom...

Tài liệu Báo cáo khoa học: Calcium signalling by nicotinic acid adenine dinucleotide phosphate (NAADP) doc

... followed by recruitment of InsP3Rs and RyRs by CICR. Althoughthere is much circumstantial evidence from physiologi-cal and pharmacological studies that cholecystokininincreases NAADP and cADPR ... release by cyclic ADP-ribose and calmodulin. J Biol Chem 270, 9060–9066.7 Galione A (2002) Regulation of synthesis of cADPR and NAADP. In Cyclic ADP-Ribose and NAADP:Structures, Metabolism and ... ampliﬁed by InsP3Rs and RyRs the on the ER by CICR[22,34,36,38]. This model accounts for the ﬁnding insome cells that localized NAADP-induced signals per-sist in the presence of InsP3Rs and...

Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate speciﬁcity by replacement of Asn149 with Asp docx

... Insights into the interaction of human arginase II withsubstrate and manganese ions by site-directedmutagenesis and kinetic studiesAlteration of substrate speciﬁcity by replacement of Asn149with ... betweenarginine and agmatine. Agmatine, which results from decarboxylation of arginine by arginine decarboxylase,is a metabolic intermediate in the biosynthesis ofputrescine and higher polyamines and may ... the interaction of human arginase IIwith substrate and metal ions was examined by site-directed mutagenesis and kinetic studies. Selection oftarget residues (Asp149, His120 and His145) was basedon...

Tài liệu Báo cáo khoa học: "Faster Parsing by Supertagger Adaptation" pptx

... (76,739,723 sentences) from the MEDLINE abstracts tokenised by McIn-tosh and Curran (2008). These sentences werePOS-tagged and parsed twice, once as for thenewswire and Wikipedia data, and then again, ... adapted to suit the parser by train-ing on the combination of these sets and the stan-dard training corpora. We applied standard evalu-ation metrics for speed and accuracy, and exploredthe source ... Budapest, Hungary.John D. Lafferty, Andrew McCallum, and FernandoC. N. Pereira. 2001. Conditional random ﬁelds:Probabilistic models for segmenting and labeling se-quence data. In Proceedings of...

Tài liệu Báo cáo khoa học: "Finding Hedges by Chasing Weasels: Hedge Detection Using Wikipedia Tags and Shallow Linguistic Features" doc

... Singapore, 4 August 2009.c2009 ACL and AFNLPFinding Hedges by Chasing Weasels: Hedge Detection UsingWikipedia Tags and Shallow Linguistic FeaturesViola Ganter and Michael StrubeEML Research ... (Lakoff, 1973; Hyland, 1998).Many NLP applications could beneﬁt from identifying linguistic hedges, e.g. question an-swering systems (Riloff et al., 2003), informationextraction from biomedical ... are simply cateringto public demand.(2) therefore America is viewed by some {{weasel-inline}} technologyplanners as falling further behindEurope 4 Data and AnnotationWeasel tags indicate...

Tài liệu Báo cáo khoa học: "Acceptability Prediction by Means of Grammaticality Quantiﬁcation" doc

... final GI of the VP reaches a high value. Itbenefits on the one hand from its own quality (in-dicated by PI) and on another hand from that ofits embedded constituents. In the end, the final GIobtained ... characterization and is formed by the subset of the satisfied properties, noted P+, and the set of the violated ones, noted P−.For example, the characterizations associated tothe NPs “the book” and “book ... set of sentences onthe one hand the grammaticality index (done auto-matically), on the basis of a PG grammar, and onthe other hand the acceptability judgment given by a set of subjects. This...

Tài liệu Báo cáo khoa học: "Tagging English by Path Voting Constraints" pptx

... text from the Wall Street Journal and the Brown Corpora. Our results from the Wall Street Journal Corpus indicate that with 400 statistically derived constraint rules and about 800 hand-crafted ... average), and corre- sponding testing texts (with about 11,800 words on the average), and then tagged these texts using the statistical rules and hand-crafted constraints. The hand-crafted ... directed acyclic graph 2 Tagging by Path Voting Constraints We assume that sentences are delineated and that each token is assigned all possible tags by a lexicon or by a morphological analyzer....

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