Báo cáo khoa học: Puriﬁcation, microsequencing and cloning of spinach ATP-dependent phosphofructokinase link sequence and function for the plant enzyme pdf

... spinach ATP-dependent phosphofructokinase link sequence and function for the plant enzyme Christian Winkler, Britta Delvos, William Martin and Katrin HenzeInstitute of Botany III, University of Du¨sseldorf, ... [22] reported the existence of chloroplast- and cytosol-speciﬁc isoenzymes in spinach. Since then, the isoforms of ATP-PFK from various plant sources havebeen studied [23–34]. Spinach cytosolic ... its importance in plant metabolism, no sequences of higher plant ATP-dependent phosphofructokinase (EC 2.7.1.11) are annotated in the databases. We have puriﬁed the enzyme from spinach leaves 309-fold...

Báo cáo khoa học: Puriﬁcation, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt

... to the anchor sequence combined to the 3¢-end of the oligo-dT primer for first-strand synthesis of cDNA [12], and 5¢-RACE amplification was performed with a gene-specificprimer based on the sequence ... in the sequence (Fig. 3). Genomic PCR experiments with a variety of different primers designed from the cDNA sequence resulted in the amplification of bands in each case.Sequencing of these bands ... aproductivity of 0.072 lmolÆmin)1Æ(mg protein))1 .The optimal pH of P. hilaris cellulase was 5.5, close to the pH in the midgut of P. hilaris larvae. The N-terminal amino-acid sequence of the purified...

Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... fromcleavage of the signal peptide and the propeptide,which happens in the maturation process for everyconotoxin [8], the removal of the two additional resi-dues Leu and Val at the C-terminus is rather ... with the presence of ashort 310-helix. A random coil rather than a compactloop in the region of residues 1–10 existed inl-Phe13-conomarphin.Fig. 3. The overlay of the backbone atoms for the ... cDNA and the deduced precursor sequence of conomarphin. The signal pep-tide is shadowed and the mature peptide isunderlined. The polyA signal AATAAA in the 3¢-UTR is also underlined. The cDNA of...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc

... Genes, enzymes and regulation of arginine biosynthesis in plants. Plant Physiol Biochem(in press).19 Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant ... CLGAT5a for the first PCR and CLGAT5b for the second PCR. For 3¢-RACE, single-stranded cDNA wassynthesized using First-Strand cDNA Synthesis Kit (AmershamBioscience) with a NotI-d(T)18bifunctional ... clone of the GAT, gene-specific primers were designed from the sequence of watermelon EST AI563351 and used for 5¢- and 3¢-RACE. The cloned cDNA encoded a proteincomposed of 460 amino acids. The...

Tài liệu Báo cáo khoa học: Puriﬁcation and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt

... middle and C-terminalregion of the HdEG66, respectively, from the sequence similarity to the termite cellulase. Then, a forward primer F1was synthesized on the basis of the N-terminal sequence of the ... sides of the cDNAsindicateprimersusedforthePCR.LengthsofthecDNAsareshown in the parentheses. (B) Structure of the genomic fragment,Hdcel-1 DNA. Open and closed boxes represent exons and introns,respectively. ... enco-ding for C-terminal region of the HdEG66 was ampliﬁed. The Hdcel-1 DNA consisted of four exons and three introns(Fig. 4B) and the coding sequence of the exons wasconsistent with that of cDNA....

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... constit-uents of the cell surface. They are found at the outermostends of the sugar chain of animal glycoconjugate. They actas an important component of the ligands recognized by the lectins. Recognition ... gently at room temperature for 3–4 h. The degree of coupling was checked by the reduction of BSM in the coupling medium. The protein concentration in the mediumbefore and after coupling was estimated ... De-O-acetylation of glycoproteins was performed following the procedures of Sarris and Palade [30] and Schauer [6]. Asolution of 750 lL of glycoprotein (5 mgÆmL)1) was addedto 250 lLof0.04M of NaOH,...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulﬁde reductase from methanogenic archaea pdf

... physiologicalelectron acceptor of the enzyme. The enzyme was notoxidized by the heterodisulﬁde of coenzyme M and coenzyme B, or by the homodisulﬁdes of these twocoenzymes. Also potassium trithionate and sodium ... out in the presence of one of the substrates of the enzyme. The physiological electron acceptor of the A. ful-gidus enzyme is still unknown. Therefore, no substrate couldbe added to the titration ... Further studies are necessary toelucidate the nature of the extractable heme present in this enzyme. The oxidation of the heme groups of the enzyme byvarious compounds was tested to identify the...

Báo cáo khoa học: Puriﬁcation and characterization of zebraﬁsh hatching enzyme – an evolutionary aspect of the mechanism of egg envelope digestion pot

... kDa product.Furthermore, their 36.5 kDa bands became broaderthan that for the 50 min incubation. The sequence analyses of the 36.5 kDa bands revealed that twosequences of further digested products ... wheremost of cross-links are located, and swelled the eggenvelope. Therefore, the manner of egg envelope diges-tion is analogous between ZHE1 and MHCE. The cross-species digestion experiments and the experiments ... ancestral form of egg envelopedigestion, and the HCE-LCE system comprises a moredeveloped form. HCE and ZHE1 would inherit the character of the ancestral enzyme with respect to the swelling of egg...

Báo cáo khoa học: Puriﬁcation and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt

... three of them were N-terminally sequenced. The observed microhet-erogeneity in the N-terminal and cDNA sequences reveals the presence of several distinct cysteine proteinase isoforms in the latex ... content in their latex; and (2) the pres-ence of other cysteine proteinases or isoforms in the latex. To investigate these two hypotheses, the proteinconcentration per milligram of dried latex, ... the other sequences. The MP tree reveals threemajor clusters. Cluster I combines the ﬁve chymo-papain isoforms of C. papaya with VXH-C, VS-B,CC-III and CC23. These results conﬁrm the hypothesisof...

Báo cáo khoa học: Puriﬁcation and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot

... cDNA sequence and sequence alignment of Cyn d 24. (A) Nucleotide and deduced amino acid sequences of Cyn d 24. The numberson the right of the ﬁgure indicate the positions of the nucleotide sequence. ... this partial sequence, two speciﬁc primerswere designed and used together with the anchor pri-mer AP2 to obtain the 5¢ and 3¢ portions of the Cyn d 24 cDNA. The nucleotide sequence of the Cyn d ... on the basis of the amino acids of number 4 and number 5 peptidesequences (Fig. 2B), and an antisense primer, designedon the basis of the amino acids of the number 2 pep-tide sequence (Fig....

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