Báo cáo khoa học: Identiﬁcation and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot

... which is involved in determining DNA binding speciﬁcity, is identical tomost members of nuclear receptor subfamily I, forinstance retinoic acid receptor (RAR) and vitamin D3 receptor. In a ... a mammalian cellreporter gene assay in in vivo results.Experimental proceduresParasites The NMRI strain of S. mansoni was maintained in snails(Biomphalaria glabrata) and Syrian golden hamsters(Mesocricetus ... Sequence alignment. (A) Alignment of DNA binding domain (C domain) and itsC-terminal extension. (B) Alignment of ligandbinding domain (E domain) (after helix 2).Helices as described in [60] are...

Báo cáo khoa học: Identiﬁcation and characterization of a collagen-induced platelet aggregation inhibitor, triplatin, from salivary glands of the assassin bug, Triatoma infestans ppt

... Identiﬁcation and characterization of a collagen-inducedplatelet aggregation inhibitor, triplatin, from salivaryglands of the assassin bug, Triatoma infestansAkihiro Morita1, Haruhiko Isawa2, ... have been reported in the saliva of blood-sucking arthropods. Among them, pallidipin and moubatin are similar to triplatin in their inhibitoryproperties. Like triplatin, pallidipin and moubatin ... respectively.Fig. 2. Western blot analysis of triplatin-1 and -2 from the salivarygland of T. infestans. Extracts from a pair of salivary glands, recom-binant triplatin-1 and recombinant triplatin-2...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... likelythat sialic acid-binding lectins in crab may recognize and bind to such organisms that contain sialic acid [12]. Lectinshave the ability to agglutinate bacteria [56,57], interact withmicroorganisms ... linkage. The sialic acid afﬁnity of the Paratelphusa lectin wasfurther proved by its inability to inhibit sialidase-treatedBSM and bovine thyroglobulin. Also the lectin failed toagglutinate desialylated ... rich in organic decomposing matter, contain-ing sialic acid in microbial polymers with which it wouldhave interacted to develop an innate immunity.Invasion of parasite, repeated injury, and...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebraﬁsh, Danio rerio docx

... STAT protein inter-action domain, STAT protein all-alpha domain, STATprotein DNA-binding domain and SH2 domain of stat6, and the zinc-ﬁnger domain, leucine-zipperdomain and fork-head domain ... of 80–100 amino acids forming a motif that is critical forDNA binding and nuclear localization), which isshared by all FOX proteins, a leucine zipper domain and a C2H2 zinc ﬁnger domain, both of which ... N-terminal STAT pro-tein interaction domain, which strengthens interactionsbetween STAT dimers on adjacent DNA-binding sites, a coiled-coil STAT protein all-alpha domain, which isimplicated in...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf

... motifs that arecommon to R-Smads, such as a nuclear localizationsignal and a DNA-binding domain in the MH1 domain and the L3 loop, and the C-terminal, receptor phos-phorylation motif in the ... size in bp, domain size in bp and domain size in amino acids are indicated at the bottom of each schematic representation of the gene, cDNA, and protein, respectively. The schematic representation ... pres-ence of a short linker region comprising only 42 aminoacids. The N-terminal MH1 domain of SmSmad1Bconsists of 137 amino acids, and comprises a conserved nuclear localization signal and a b-hairpin...

Báo cáo khoa học: Identiﬁcation and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf

... Cloning and sequencing of thiol-specificantioxidant from mammalian brain: alkyl hydroperoxidereductase and thiol-specific antioxidant define a largefamily of antioxidant enzymes. Proc Natl Acad ... during aero-bic respiration. In contrast with Prxs discovered in the aerobichyperthermophilic archaeon A. pernix and the anaer-obic hyperthermothilic archaeon P. horikoshii, the ana-lysis of ... 731 Identification and characterization of 1-Cys peroxiredoxinfrom Sulfolobus solfataricus and its involvement in the response to oxidative stressDanila Limauro1, Emilia Pedone2, Luciano Pirone2and...

Báo cáo khoa học: Identiﬁcation and characterization of B¢¢-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues Evidence for an independent N-terminal splice variant of PR130 and an extended human PR48 protein pot

... height,proving that no internal methionine was used as initiationstart in the translation of the wild type mRNA of XN73 and that the XN73 protein runs with an aberrant molecular mass in SDS/PAGE. It was ... TTGCAAgtatgc 3811 tttcagACCCTA8 157 ACCAGGgtaagt 5461 atttagCTTCAT9 49 AACAAGgtaaga 2646 ctttagGGGAAA10 90 TACCAGgtatga 11341 atctagCATTGA11 177 TTGATGgtgaga 1075 atgtagGCAAAA12 119 CAGAAGgtaaca ... propor-tion of PP 2A can also exist in vivo as a dimer [5]. The association of a B subunit with the AC core dimer can result in altered substrate speciﬁcity, catalytic activity and subcel-lular localization....

Báo cáo khoa học: Identiﬁcation and characterization of plasma kallikrein–kinin system inhibitors from salivary glands of the blood-sucking insect Triatoma infestans pptx

... that both triafestin-1 and triafestin-2 inhibitedkallikrein–kinin system activation by inhibiting the reciprocal activation of FXII and PK without affectingtheir amidolytic activities. Activation ... pathway.Triafestin inhibits FXII and PK reciprocalactivation and bradykinin generationTo investigate inhibition of the kallikrein–kinin systemby triafestin-1 and triafestin-2, the effects of triafestin-1 and ... suggestthat triafestin-1 and triafestin-2 inhibit activation of the kallikrein–kininsystem by interfering with the association of factor XII and high molecularweight kininogen with biological activating...

Báo cáo khoa học: Identiﬁcation and characterization of oxidized human serum albumin A slight structural change impairs its ligand-binding and antioxidant functions pptx

... Sonaka1, Eiichiro Suzuki2 and Kazuo Hirayama21 Pharmaceutical Research Laboratories, Ajinomoto Co. Inc., Kawasaki, Japan2 Institute of Life Science, Ajinomoto Co. Inc., Kawasaki, JapanHuman serum ... albumin A slight structural change impairs its ligand-binding and antioxidantfunctionsAsami Kawakami1,*, Kazuyuki Kubota2,*, Naoyuki Yamada2, Uno Tagami2, Kenji Takehana1,Ichiro Sonaka1, ... investigated the relative binding proper-ties of these two types of HSA. We investigated the binding of L(small)-Trp as an endogenous ligandwhich binds to site II, and of cefazolin (site I- ligand)and...

Báo cáo khoa học: Identiﬁcation and characterization of four novel peptide motifs that recognize distinct regions of the transcription factor CP2 doc

... Wilanowski T, Cerruti L, Zhao LL, Cunning-ham JM & Jane SM (2003) The identiﬁcation and char-acterization of human Sister -of- Mammalian Grainyhead(SOM) expands the grainyhead-like family of develop-mental ... N-terminal transcriptionalactivation (amino acids 1–63), N-terminal Elf-1(amino acids 63–244), the basic (amino acids 244–250), SPXX (amino acids 250–403), Q ⁄ P (amino acids403–413) and C-terminal ... that bind to CP2 shows a preponderance of histidines, implying the importance of this amino acid residue in the CP2-binding motif of proteins that interact with CP2. Indeed, two orthree histidine...

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