... delete site I), C and D (to delete site II) , E and F (to delete site III), G and H (to delete the perfect inverted repeat) and I and F (todelete simultaneously sites II and III) were ligated ... Further single replacementswithin each binding site (I, II or III) may reveal the essential role of single nucleotides in the specific inter-action with SenR. The position of the SenR operatorG. ... pET21a, and sub-sequently transformed into E. coli XL1-Blue. Havingsequenced several of the resulting plasmids pETR1, pET-R1D60A or pETR1D65A, we verified the correctness of the senR gene and the...