Báo cáo khoa học: DNA polymerase e associates with the elongating form of RNA polymerase II and nascent transcripts pot

Tài liệu Báo cáo khoa học: KCNE4 can co-associate with the IKs (KCNQ1–KCNE1) channel complex ppt

... lysates werederived from the cerebellum. Colon lysates were derived from the descending colon. Heart lysates were derived from the left ventricle.Muscle lysates were derived from skeletal muscle ... the plasma mem-brane in the presence of KCNQ1. All lysates were probed with anti-HA to demonstrate the presence of KCNE4. (C) KCNQ1 trafﬁcs to the plasma membrane in the presence of KCNE1 and ... lanes 7 and 8),no cross-reactivity was observed between the respectiveantibodies. These experiments offer conclusive evidencethat KCNE4 forms channel complexes with KCNQ1in vitro. The suppression...

Tài liệu Báo cáo khoa học: "Enhanced word decomposition by calibrating the decision threshold of probabilistic models and using a model ensemble" pdf

... counts presented in the 2 × 2 contingency table of Figure 1. For gen-eral evidence, we averaged across all experimentsusing relative frequencies. Note that the relativefrequencies of positives (TP ... are often considered as the smallest unit of a language when examining the grammaticalstructure or the meaning of sentences, referred toas syntax and semantics, however, words them-selves ... PROMODES -E gives better results than the individual modelsthemselves and therefore manages to leverage the individual strengths of both to a certain extend.However, can we pinpoint the exact contributionof...

Tài liệu Báo cáo khoa học: "NATURAL VS. PRECISE CONCISE LANGUAGES FOR HUMAN OPERATION OF COMPUTERS: RESEARCH ISSUES AND EXPERIMENTAL APPROACHES" doc

... the expectation that the second time the task is per- formed the subject does better, half the subjects begin with natural language, while half the subjects begin 14C with the alternative ... ' ~e are computer workers and therefore well placed to know the present and future dangers of computer systems. They are used to classify, control and to repress.") reveal the anger and ... called repeated measures) designs are effective. Su:,~ects perform the task with each mode and the statisical tests compare scores in one mode against the other. To account for learning effects,...

Báo cáo khoa học: Nanoparticles can induce changes in the intracellular metabolism of lipids without compromising cellular viability docx

... protected or if they degradewithin the biological environment. We have studied the effects of QDs on living cells and have reportedtheir internalization, the intracellular production of reactive ... of CdTe QDs. (E) The mean number of lipid droplets per microscopic ﬁeld evaluated asdescribed in Materials and methods. The data represent the mean and SEM from two independent experiments (n ... unique luminescence char-acteristics and the potential to become attractive tools for medical imaging.However, some of these particles can cause oxidative stress and induce celldeath. The objective...

Báo cáo khoa học: C ﬁ G base mutations in the CArG box of c-fos serum response element alter its bending ﬂexibility Consequences for core-SRF recognition potx

... candeduce that 10 °C corresponds closely to the tempera-ture transition between the bent form and the linear form, since their populations are roughly equivalent. The agreement between our results ... stiffnessexperience part of the SRE internal movements. If the conversion between a bent and a linear form occurs ata shorter or longer timescale than the fluorescenceexcited state of fluorescein (4 ns), the ... specific complexformation. They appear to be as important as the con-servation of the DNA base sequence. Therefore, the basic question is no longer what prerequisite site on the DNA determines...

Tài liệu Báo cáo khoa học: DNA methylation-mediated nucleosome dynamics and oncogenic Ras signaling pptx

... depletion of H3K4Me2 in the regions of DNA methylation. Conversely, the level of H3K4Me2 remains high in the unmethylated DNA regions regardless of the presence of RNA Pol II. It can be proposed thatMeCpG- ... promoters of silenced gene during cancer development [16]. Catalyticmechanisms of DNMTs involve the formation of acovalent bond between a cysteine residue in the activesite of the enzyme and ... example of the emerging ﬁeld of pharmacoepigenomics. The impact of chemotherapy would be dependent on the epigenetic state of cardinal genes such as MGMT[1,41]. Knowing the state of methylation of...

Tài liệu Báo cáo khoa học: DNA adenine methylation changes dramatically during establishment of symbiosis pdf

... MluI, and NotIaslandmark enzymes. These four enzymes cleave speciﬁcGC-rich sequences and therefore gave satisfactoryresolution of a sufﬁcient number of spots because the genomes of all three bacterial ... The cleavage by the landmark enzymes(AscI, MluI, and NotI) is not affected by CcrMmethylation because of the lack of GANTC sequenceson their recognition sequences; therefore, the spotintensity ... fragmentation enzyme. There are 1071 informative spots in the pattern. (B) Virtual RLGS pattern calculated based on the whole-genome sequence. The numbers to the right of the spots correspond to the sequence...

Tài liệu Báo cáo khoa học: DNA modiﬁcation with cisplatin affects sequence-speciﬁc DNA binding of p53 and p73 proteins in a target site-dependent manner pptx

... depending on the nucleotide sequence of the given target site. The relative sensitivities of protein DNA binding to cisplatin DNA treatmentcorrelated with the occurrence of sequence motifs forming ... 2A) whosemobilities reﬂected different molecular weights of the p73 isoforms. To verify the speciﬁcity of the bandshifts for DNA complexes with the proteins studied,we used the band supershift ... manifested by differ-ent intensities of the R bands). To eliminate thesedifferences, the effects of DNA cis-platination on the protein DNA interactions were always normalized with the intensity...

Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by ﬂuorescence resonance energy transfer and the role of ATP hydrolysis pptx

... barriers in the reaction pathway. The FRET chan-ges observed were indeed related to stable changesassociated with DNA in the strand exchange reactionas they were stable to the steps of protein ... line 3) reflects genuine FRET change associated with strand renaturation.Homology dependence of renaturation activityIn the following experiment, we examined the homol-ogy dependence of the renaturation ... confirm that the increasein the fluorescence signal is specific to the emissionmaximum of fluorescein, the donor dye in the FRETpair, we compared the emission spectra of the strandexchange reaction...

Báo cáo khoa học: DNA-binding characteristics of the regulator SenR in response to phosphorylation by the sensor histidine autokinase SenS from Streptomyces reticuli doc

... delete site I), C and D (to delete site II) , E and F (to delete site III), G and H (to delete the perfect inverted repeat) and I and F (todelete simultaneously sites II and III) were ligated ... Further single replacementswithin each binding site (I, II or III) may reveal the essential role of single nucleotides in the speciﬁc inter-action with SenR. The position of the SenR operatorG. ... pET21a, and sub-sequently transformed into E. coli XL1-Blue. Havingsequenced several of the resulting plasmids pETR1, pET-R1D60A or pETR1D65A, we veriﬁed the correctness of the senR gene and the...

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