... ligase). The multiubiquitinatedtarget proteins thus produced are degraded by the 26Sproteasome. On the other hand, some proteins, such asornithine decarboxylase, are degraded by the proteasomewithout ... Takentogether, the results suggest that the BTG/Tob familymembers BTG1, BTG2, Tob, and Tob2 are degraded by the 26S proteasome.BTG/Tob family proteins are multiubiquitinatedTo determine whether ... respectively, the PCR products subcloned in the pGEM-T-vector (Pro-mega) were digested with EcoRI and SalI and theninserted into the EcoRI and SalI sites of the pCI-neo-T7-vector (38). The terminal-truncated...