Báo cáo khoa học: DNA-binding characteristics of the regulator SenR in response to phosphorylation by the sensor histidine autokinase SenS from Streptomyces reticuli doc
... 2007 The Authors Journal compilation ª 2007 FEBS 3913
DNA-binding characteristics of the regulator SenR in
response to phosphorylation by the sensor histidine
autokinase SenS from Streptomyces reticuli
Gabriele ... characterization of the
DNA-binding properties in response to phosphoryla-
tion by the sensorkinase SenS.
Results
Cloning...
... neigh-
bouring monomer in the tetramer. By contrast, the
side chain of the mutated residue (Val376) points
toward the inside of the helix–loop–helix structure.
Consequently, the side-chain atoms of Val376 ... Determination of full-length cDNA
sequences using the RACE method in both strains
revealed the presence of a single point mutation in
1337 nucleotides...
... in am-
matory disease that occurs in about 1% of the popula-
tion. The in ammatory process is characterized by
in ltration of in ammatory cells into the joints, leading
to the proliferation of ... chemokines stimu-
lated mainly by in ammatory cytokines, including IL-1b, TNF-a and
IFN-c. (B) Chemokines expressed in the joint recruit leukocytes into
the joints....
... to the pK
a
of
His210, is mainly determined by the type of side-chain at
residue 169. In the Trp169 mutants, His210 deprotonates at
a lower pH compared to that for wild-type API, due to the
increased ... size of the side-chain at residue 169 and the pK
2
showed a clear linear relationship (Fig. 3B). The pK
2
increased as the size of the residue at position...
...
attempt to find fillers for the descriptor slots of the
temp]ate in question, and a third argument which re-
presents the output of the procedure, i.e., the in-
stantiated slots.
The body of the ...
flects the information user's conceptualization of the
domain, i.e., his view of what that class of entities
involves. In the domains under inves...
... the N-domain
and C-domain (indicated by the arrow in Fig. 1A).
Thus, information on the selectivity of both the sub-
strate and the inhibitor for the N-domain and
C-domain can be determined from ... the binding of RXP407
to the N-domain led to 80% inhibition of its activity
(arrow in Fig. 1A, closed circle), whereas in the rat
enzyme binding of RXP407...
... presently of the nature of the FtsY–membrane
interaction in Archaea. Moreover, the steps leading to
deliveryofSRPtothearchaealmembranehaveyettobe
described.
Towards an understanding of the membrane interaction
of ... the ability of
either FtsY or the FtsY NG domain to bind to the
membrane, with floatation of the proteins to the upper
gradient fractions o...
... Within a region of a cubic
phase in the diagram an increase in the temperature
generally results in a decrease in the size of the unit cell.
This is interpreted to be an effect of an increase in ... present in the MO bilayer, the shorter tail will only affect
the part of the MO molecule from the head group to the
double bond, leaving the rest o...
... fragment from K. pneumoniae M5a1 into
pBSK
+
vector creating pRS167, followed by insertion of
thetetracyclineresistancecassetteintotheEcoRV site of
fdnG fragment resulting in pRS177. The 3.2-kb ... transferred from DMNH
2
to the FAD-cofactor of
NifL (Fig. 4A). The reduction of NifL-bound FAD by a
quinol derivative was confirmed using menadiol that also
resulted in r...
... of RNase molecules
on the cell surface), thereby favoring their endocytosis
[19]. The stoichiometry of RI binding to RATEs and
the role of the RI–RATE complex in the in vitro inacti-
vation of ... Unfortunately, the cytotoxicity of these RNases
is limited by several factors at the cellular level [8],
including restricted internalization into the cell, release...