... 2007)
doi:10.1111/j.1742-4658.2007.05704.x
The two forms of monoamine oxidase, monoamine oxidase A and mono-
amine oxidase B, have been associated with imidazoline- binding sites (type
2). Imidazoline ligands saturate the imidazoline- binding ... Interactions of imidazoline ligands with the active site of
purified monoamine oxidase A
Tadeusz Z...
... assay
endpoints were used in this case: glycosylase activity
was measured after full thermal degradation of the AP
site left by base excision, whereas the AP lyase activity
was assayed as unassisted ... that
electrostatic interactions may stabilize catalytically
inactive conformations of the enzyme in the case of
8-oxoG :A.
Unlike 8-oxoG:C pairs that exist in a convention...
... 2006).
They used a natural language tagger which was
trained on the output of ParaMor and Morfes-
sor. The goal was to mimic each algorithm since
ParaMor is rule-based and there is no access ... Background
In the past years, there has been a lot of inter-
est and activity in the development of algorithms
for morphological analysis. All these approaches
have in common that th...
... et al.
[44]; they contain a CdSe core, their diameter is 2.4 nm and
they have an emission maximum of 518 nm. The CdSe core
was capped with one layer of ZnS to which cysteamine was
attached, and ... Shvedova AA, Castranova V, Kisin ER, Schwegler-
Berry D, Murray AR, Gandelsman VZ, Maynard A &
Baron P (2003) Exposure to carbon nanotube material:
assessment of nanotube cytotoxi...
... decomposition
analysis of Raman spectra
The Raman spectra of the three duplexes are sensi-
tive to temperature variations between 10 and 65 °C.
To find out the basic character of these changes,
each set of ... 415–419.
58 Arai S, Chatake T, Ohhara T, Kurihara K, Tanaka I,
Suzuki N, Fujimoto Z, Mizuno H & Niimura N (2005)
Complicated water orientation in the minor groove of...
... the understanding of the role of KCNE4 as a
potentially important regulator of KCNQ1 and other
K
V
channels.
Results
Characterization of KCNE4 antibody
A rabbit polyclonal antibody raised against ... & Greger R (1999) Carbachol activates a
K
+
channel of very small conductance in the basolater-
al membrane of rat pancreatic acinar cells. Pflugers
Arch 438, 597–603....
... hippocampus, substantia
nigra and striatum. Sensory neurons of rat dorsal root
ganglia are also able to produce anandamide in high
amounts [42].
Degradation
Theoretically, intracellular anandamide ... G .A. , Finazzi-Agro, A. , Vliegenthart,
J.F. & Maccarrone, M. (2002) Oxygenated metabolites of ana-
ndamide and 2-arachidonoylglycerol: conformational analysis and
interaction with...
... prepared and analysed
by 3–12% gradient SDS/PAGE. The gel was dried and the radioac-
tivity was visualized with a Bio-Imaging analyser BAS-2000. Lanes 1–3
represent extracts of epithelial cells and ... bacteria. (A)
Epithelial cells and fibroblasts were labelled with [
35
S]sulfate, washed
and incubated with chondroitinase ABC and HS lyase. Triton extracts
of untreated and enz...
... that
A
B
C
shifted
123
123456
band
Probe
1.00E+008
AAAGAGATG
AAGGACATG
AAAGACCTG
AAAGACATG
K
d
=1.2 nM
AAAGAGATG
K
d
=0.29 nM
AAAGACACG
K
d
=4 nM
AAAGACACG
AAAGACATA
AGAGACATG
AAAGACATG
AAAGCCATG
AAATACATG
8.00E+007
6.00E+007
4.00E+007
2.00E+007
0.00E+000
14
16
12
10
8
Fluoriscence
340
Fluoriscence
340
6
4
0.00 ... EMSA
Fluorescence quenching
Result Average K
d
(nM)
AAAGACATG + + 0.25
A
G...
... F, Cazzola G, Consolini R, De Mattia D,
Dell’Erba G et al. (2002) Clinical, immunological, and
molecular analysis in a large cohort of patients with
X-linked agammaglobulinemia: an Italian multicenter
study. ... or SYK themselves, is capable
of transforming NIH-3T3 cells [70]. In addition, we
and others have demonstrated that the activation and
plasma membrane localization of the...