Báo cáo khoa học: Coordination of three and four Cu(I) to the a- and b-domain of vertebrate Zn-metallothionein-1, respectively, induces signiﬁcant structural changes doc

Báo cáo khoa học: Tetracysteine-tagged prion protein allows discrimination between the native and converted forms pptx

... arsenic atoms in FlAsHenables it to bind with high affinity and specificity to the TC motif introduced into a variety of different proteins[15]. The binding of FlAsH reagent and also the quan-tum ... ¼ðPrPtotÀ PrPnatÞ=PrPtotwhere PrPnatrepresents the nonconverted amount and PrPtot the total amount (converted plus nonconverted) of PrP.Construction of retroviral plasmids, production of retrovirions ... method to determine the fraction of converted PrP, based on the ability of the fluorescein arseni-cal helix binder reagent to differentiate between the monomeric and fibril-ized form of TC-tagged...

Báo cáo khoa học: Epidermal growth factor receptor-regulated miR-125a-5p – a metastatic inhibitor of lung cancer potx

... subset of human lung cancers To gain further insights into the role of miR-12 5a-5 pin lung carcinogenesis and to examine the clinical rele-vance of our findings, we investigated the expression of ... added to the insert. Forty-eight hours afterseeding, the cells on the upper surface of the membranewere removed using cotton buds. Cell monolayers on the lower surface of the insert were fixed and ... gefitinib. The value for miR-12 5a-5 p in the EGF group was set at 1, and the relative amounts of miR-12 5a-5 pin the other groups were plotted as fold induction.G. Wang et al. MiR-12 5a-5 p inhibiting...

Báo cáo khoa học: "Entailment-based Text Exploration with Application to the Health-care Domain" pot

... common type of entailmentrules, in which the left-hand-side of the rule (LHS) and the right-hand-side of the rule (RHS) are propo-sitional templates - a proposition, where one or both of the arguments ... Fragment of the entailment graph for ‘asthma’(top), and its conversion to a DAG (bottom).3.2 Exploration Scheme The objective of the exploration scheme is to supportquerying and offer facets ... the roots of the DAG. As a starting point, only the roots of the DAGare displayed to the user. Figure 4 shows the ﬁveroots for the ‘asthma’ query.Exploration process The user selects one of...

Báo cáo khoa học: Coordination of three and four Cu(I) to the a- and b-domain of vertebrate Zn-metallothionein-1, respectively, induces signiﬁcant structural changes doc

... stoichiometries of three and four for the a- and b-domain, respectively. The presence of Cu(I) led to dramatic conformational changes of both polypeptide folds. Cu(I) stoichio-metries of up to six Cu(I) ... mm. Coordination of Cu(I) to acetonitrile prevented undesired Cu(I) disproportionationinto Cu(II) and elemental copper. Four and three molarequivalents of Zn(ClO4)2were added to the a- and b-domains, ... Analysis of the NOESY and TOC-SY (not shown) spectra of the two domains permitted the full sequence-speciﬁc assignments, the identiﬁcation of the spin systems and the assignment of 398 and 252 of the...

Tài liệu Báo cáo khoa học: 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5¢-phosphate synthases of fungi and archaea docx

... wereshown to be A-type reductases catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1¢ (S) position of the substrate. Theseresults are in agreement with the known three- dimensional ... temperature, and a dualset of 13C-NMR signals is therefore observed foreach of the 13C-labelled carbon atoms of the ribosylside chain [12]. Moreover, the signals of the side-chain carbon atoms of 13C-labelled ... reduction of the ribosyl side chain of compound 3 in the ascomy-cete Ashbya gossypii is conducive to introduction of ahydrogen atom into the 1¢-proS position of the ribitylside chain of riboﬂavin...

Tài liệu Báo cáo khoa học: DNA modiﬁcation with cisplatin affects sequence-speciﬁc DNA binding of p53 and p73 proteins in a target site-dependent manner pptx

... in the genome of living cells is naturally stochastic on the one hand, and inﬂuenced by the actual structural and functional state of the given chromatin domainon the other. Moreover, some of the ... Hence, the response of a cancer cell to cisp-latin seems to be rather complex, and its relationship to the status of the p53 family proteins does notappear to be straightforward. The results of ... GGG and AG motifs in one strand and GG and GTG motifs in the other, thus offering not only the highest total number of reactive motifs among the p53DBSs tested, but also the highest number of...

Tài liệu Báo cáo khoa học: Efﬁcient RNA ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes consisting of conventional and reverse-joined hairpin ribozyme units ppt

... Addition of the fragment S20 cp led to the for-mation of new products detected as three additionalbands (lanes 3 and 4). These signals correspond to the 29- and 35-mer resulting from ligation of the ... the oxygen atom of the free 5¢-OHgroup of one RNA fragment attacks the phosphorous of the cyclic 2¢,3¢-phosphate group of another, result-ing in ligation of the two fragments. In contrast to ... elucidating the mechanism and structure of catalytic RNA molecules, and we are now beginning to understand ribozymes well enough to turn them into useful tools. Work inour laboratory has focused on the...

Tài liệu Báo cáo khoa học: Coordination chemistry of iron(III)±porphyrin±antibody complexes In¯uence on the peroxidase activity of the axial coordination of an imidazole on the iron atom ppt

... about the topology of the binding site of the antibody and particularly to appreciate the size of the cavity left around the iron atom and which kind of ligands it can accommodate; second, to measure ... in the presence of increasing concentra-tions of imidazole and compared to that of the a,a -and a,b-1,2-Fe(DoCPP ))13G10 complexes. With both a,b -and a ,a-1 ,2-Fe(DoCPP), the rate of oxidation of ... imidazole ligands, that of the Fe(ToCPP))13G10complex is able to bind only one imidazole ligand; (c) the binding of one imidazole to the iron atom inhibits the peroxidase activity of the Fe(ToCPP))13G10...

Tài liệu Báo cáo khoa học: Casein kinase 2 speciﬁcally binds to and phosphorylates the carboxy termini of ENaC subunits ppt

... between the C tails of b and c and the ubiquitin ligase Nedd4. The WW domains of Nedd4 bind to the proline-rich PY motifs of b and cENaC, leading to channel ubiquitination, internalization and degradation ... quantiﬁed byphosphorimaging of the band corresponding to the full-length protein, and normalizing to the Coomassie bluestaining of the same band.)It was next demonstrated that the kinase eluted infraction ... (Fig. 8A), the inhibitory effects of heparin and DPG (Fig. 8B), the fact that puriﬁed CK2 canphosphorylate bS631 and cT599 (Fig. 7A), the presence of the a subunit of CK2 in the active cytosolic...

Báo cáo khoa học: Caenorhabditis elegans metallothionein isoform speciﬁcity – metal binding abilities and the role of histidine in CeMT1 and CeMT2 potx

... level to be of the highest interest, in order to shed light on the possible physiological functions of MTs in thisorganism and to further the understanding of the forces driving MT isoform differentiation, ... for the native and recombi-nant Cd(II)–CeMT2 isoform [16,20]. The slight blue-shift of the spectrum of Cd6Zn1–CeMT1 in relation to that of Cd6–CeMT2 is attributable to the inﬂuence of the ... than that of CeMT2. MTs appear to compriseonly one of three strategies developed by C. elegans to prevent cadmium intoxication, with the other twoconsisting of phytochelatins [23] and the selectivepumping...

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