... transfer in cytochrome P450 reductase
FEBS Journal 275 (2008) 454 0–4 557 ª 2008 The Authors Journal compilation ª 2008 FEBS 4557
Inter-flavin electron transfer in cytochrome P450
reductase – effects of ... The role of
cysteine 566 in catalysis and cofactor binding. J Biol
Chem 266, 1997 6–1 9980.
26 Shen AL & Kasper CB (1995) Role of acidic resi...
... folded domains: an FAD- and NADPH-binding
domain related to ferredoxin-NADP
+
reductase, a flavo-
doxin-like FMN-binding domain and a connecting ÔlinkerÕ
domain. In addition to the cytochromes P450, ... kinetic
experiments to include studies of internal electron transfer
in the presence of NADPH, NADH and fragments of
nicotinamide coenzymes. We show that binding of the...
... regulation
of transferrin binding
Previous reports have established that in certain cell
types HFE acts to regulate intracellular iron levels by
decreasing the binding of transferrin to transferrin
receptors ... striking decrease in cell-surface bind-
ing of transferrin, and that little or no reduction in
transferrin binding was observed in cells expressing the
HFE-C282Y mutan...
... [26] using a single
testosterone concentration of 100 l
M
. – Indic ates rate < 0.1 min
)1
.
P450
Rate (min
)1
)
1b-OH 2b-OH 6b-OH 15b-OH
1A1 – – 1.1 ± 0.1 –
1A2 – – 6.7 ± 0.3 –
1B1 – – 7.7 ... baculovirus-infected insect cells contain-
ing 4 pmol of P450 3A4. (Varying amounts of P450 were
used because of differences in rates of the systems, i...
... plasminogen activator inhibitor and
vitronectin and evidence that the bovine inhibitor binds to a
thrombin-derived amino-terminal fragment of bovine vitronectin.
Biochim. Biophys. Acta 1078, 2 3–3 0.
24. ... overlapping binding
site on thrombin
At this point, the mechanism of the inhibitory effect of TM
on the interaction between PAI-1 and thrombin remains to
be elucidated. To...
... 1748 6–
17500.
30 Sielaff B & Andreesen JR (2005) Kinetic and binding
studies with purified recombinant proteins ferredoxin
reductase, ferredoxin and cytochrome P450 comprising
the morpholine mono-oxygenase ... potassium phosphate buffer (pH 6.3) containing
10% glycerol and 100 mm KCl, CYP175A1 was eluted with
a linear gradient of 10 0–6 00 mm KCl in 50 mm potassium
ph...
... In addition, we outline an approach of si-
multaneous machine translation utilizing linguis-
tic phenomena, flexible word order, and inversion,
characterizing Japanese speech.
2.1 Difference of ... English speech transcript
into Japanese incrementally. It is composed of
three modules: incremental parsing, transfer and
generation. In the parsing module the parser deter-
mines th...
... proteins.
a-Actinin–stFRET (A), a-actinin–GFP (B), filamin A–stFRET (C), filam-
in A–CFP (D), spectrin–stFRET (E) and spectrin–CFP (F) in 3T3 fibro-
blast cells; Collagen-19–stFRET (G) and COL-19–GFP (H) in
C. ... stretching
of the coiled-coil myosin II [44]. Schweiger et al. [45]
obtained a three-phase force–distance relationship: a
linear phase of $ 1mNÆm
)1
, a plateau of $ 25...
... cobalamin at 0 min (thick solid
line), 30 min (thin solid line), 60 min (thin long-dashed line),
120 min (thin short-dashed line), 240 min (thin dotted line) and
360 min (thick dotted line) of incubation, ... model of damaged
cofactors) for free adeninylpentylcobalamin (AdePeCbl)
(an inactive coenzyme analog containing the adenine
ring in the upper axial ligand; a model of intact co...
... C
5
-domain,
catalyzing the intramolecular nucleophilic attack of
the L-hOrn
3
a-amino group onto l-haOrn
4
. Taking
into account that the synthetases involved in the bio-
synthesis of the DKP-inheriting ... (solvent B) at a flow rate of 0.3 mLÆmin
)1
: lin-
ear increase from 0% B to 50% within 20 min followed by
a linear increase to 95% B in 5 min, holding B for an
additional 5 min...