Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf
Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf
... FEBS 4521 In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases Di Ouyang, Long Yi, Liangliang Liu, Hong-Tao Mu and Zhen XiState Key Laboratory of Elemento-Organic ... pocket, with its amino sugar anchored in the major groove of the DNA pointing toward the3¢ -bulge- flanking base pair.We were interested in small molecules that can selec-tively bind bulge DNA and ... theligand–nucleic acid complex is formed. To gain insightinto the binding of drugs to bulge DNA, several bulge- containing oligonucleotides were selected as bindinghosts for DDI-1A and DDI-1B.CD...
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Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc
... and itsbinding affinity is not altered by the presence of DNA in the DNA binding site.Interestingly, when 3 nMRNA and 10 nM DNA wereadded simultaneously to a limiting amount of protein in TK8 ... using two different preparations of protein and three different preparations of DNA and RNA substrates. Each time thesplicing reaction was minimally affected by prebinding the DNA to protein.As ... not bind the same singlebinding site, rather that I-AniI appears to contain twobinding sites.Keywords: Aspergillus nidulans; homing endonuclease;RNA binding protein; DNA sliding; RNA splicing.Group...
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Báo cáo khoa học: In vitro characterization of a plastid terminal oxidase (PTOX) pot
... properties of PTOX in vitro. In this report, we produced the protein in E. coli and studied its substrate preferences, the factors in uencing its activity and the nature of suitable metalcofactors and ... stimulatedstrongly in both membrane types (Fig. 1A,B) in thepresence of 0.2 mM of ubiquinone and, to some extent, of dPQ, duroquinone or vitamin K1 (phylloquinone). In contrast, addition of benzoquinone ... O2consumption in presence of KCNminus O2consumption in presence of nPG.Fig. 4. Comparison of a putative quinone-binding site in PTOX and AOX sequences. The potential Q site is shaded and arrows indicate...
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Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf
... hpfembryos were incubated during 15 min with different kinaseinhibitors at 30 °C and then used to analyse in vitro recom-binant CNBP phosphorylation in kinase buffer containing100 lm ATP and 5 lCi ... November2006)doi:10.1111/j.1742-4658.2006.05596.xThe zinc-finger cellular nucleic acid binding protein (CNBP) is a strikinglyconserved single-stranded nucleic acid binding protein essential for normalforebrain formation during mouse and chick ... properties of a variety of proteins with common biochemical activities, such asthe binding to single-stranded nucleic acids and theremodelling of their secondary structures.A model involving CNBP...
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Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx
... dimethylsulfoxide in aconcentration-dependent fashion.Our results indicate that in vitro c-secretase cleavage of APP substrates is inhibited by zinc and that zincincreases the apparent molecular mass of ... Thisis the first report suggesting that this metal bindingdomain is functional within APP C99 causing oligo-merization with the biochemical consequence of inhib-iting c-secretase cleavage. This ... prepared using C101-3FLAG, c-3FLAG and e-3FLAG standards with theresulting line having a correlation coefficient of 0.998. Thisline was used to predict the molecular mass of the CTFmigrating faster...
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Báo cáo khoa học: In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I pot
... signal sequence is involved in guiding the protein into the targeting and translocatingpathway by interacting with the membrane and othercomponents of the cellular secretory machinery [1]. Thesignal ... of S. pneumoniaeSPase IFor in vitro self-cleavage of S. pneumoniae SPase I in thepresence of phospholipid, 20 lL of reaction containing5 lg of purified SPase I was incubated in 37 °Cfor2hin20mMTris/HCl ... thenwashed with 50 mL of lysis buffer with 15 mMimidazole.The protein was finally eluted out with 10 mL of elutionbuffer, and 1 mL of fractions were collected and analyzedby SDS/PAGE. In vitro self-cleavage...
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Báo cáo khoa học: In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase potx
... display),increasing the likelihood of correct folding in a redu-cing environment while minimizing the risk of proteo-lytic degradation. The association of plasmid and fusion protein can be maintained ... for 10 min. In vitro selection formAb M2 binding was by adding 200-lL aliquots of thesupernatant to the selection matrix and incubating at roomtemperature for 30 min. Unselected protein–plasmid ... epitope without significantly perturbing thefolding of the underlying (ba)8-barrel. This was inves-tigated by comparing the far-UV and near-UV CDspectra of PRAI and FLAG-PRAI in a buffer in which...
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Tài liệu Báo cáo khoa học: Oocyte membrane localization of vitellogenin receptor coincides with queen flying age, and receptor silencing by RNAi disrupts egg formation in fire ant virgin queens ppt
... Streptavidin (Invitrogen;1 : 200) in blocking solution for 1 h. Sections were washed and mounted in Vectashield Mounting medium with 4¢,6-di-amidino-2-phenylindole for nuclear staining (Vector,Burlingame, ... ethanol and in water for 30 min at room temperature.After rinsing twice for 5 min with PBST (NaCl ⁄ Picontain-ing 0.05% Triton X-100), slides were incubated in blockingsolution (5% goat serum and ... nutrition and insulin signaling is inverted in honey bee adults, and JH inhibits Vg expression in adults rather than stimulating it [59,60]. The short neu-ropeptide F signaling cascade is involved in...
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Tài liệu Báo cáo khoa học: A kinetic model of the branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis thaliana doc
... validated in vitro andusedtoexaminethe branch-point kinetics in detail and to obtain insights intothe kinetic controls of methionine and threonine synthesis in plants.The branch-point between ... divergence of two fluxes (fluxes of cystathionine and threonine) with theconvergence of two fluxes (fluxes of Phser and cysteine).Interestingly, the properties of CGS are such that the flux of cystathionine ... equations.Reconstitution of the branch-pointA constant flux of Phser was obtained with purifiedhomoserine kinase in the presence of saturating concentra-tions of ATP and homoserine. Two different couplingsystems...
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Báo cáo khoa học: In vivo production of catalase containing haem analogues pot
... gallium, tin, and zinc, and also variants con-taining meso-protoheme IX, ruthenium meso-protoporphyrin IX and (metal-free) protoporphyrin IX. Engineered haem proteins of this type are of potential ... essentiallyinactive, and that this is not due to inactivation duringisolation of the protein.Light absorption spectra of the purified iron-con-taining and gallium-containing catalases are presented in ... protein, in binding porphyrins.It is not known how haem is transported intracellularly in bacteria, and the mechanisms by which haem isincorporated into soluble and membrane-bound pro-teins inside...
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