Báo cáo khoa học: The pH dependence of kinetic isotope effects in monoamine oxidase A indicates stabilization of the neutral amine in the enzyme–substrate complex ppt

... The pH dependence of kinetic isotope effects in monoamine oxidase A indicates stabilization of the neutral amine in the enzyme–substrate complex Rachel V. Dunn1, Ker R. Marshall2, Andrew ... Geneservices(Cambridge, UK) using the primers 5¢-GTCTTCGAA A CCATGGAGAATCAAGAGAAGGCGAGTATCGCGGG-3¢ and 5¢-GAGAGCTCGAGAACAGAACTTCAAGACCGTGGCAGGAGC-3¢. The NcoI and Xho I sites used forfurther cloning are ... 4048–4052.Supplementary material The following supplementary material is availableonline:Fig. S1. pH dependence of the steady-state kinetic parameters of MAO A- catalysed oxidation of benzyl- amine at 20...

Báo cáo khoa học: "You Can’t Beat Frequency (Unless You Use Linguistic Knowledge) – A Qualitative Evaluation of Association Measures for Collocation and Term Extraction" pot

... or associa-tion measures yield scores indicating to what de-gree a candidate qualiﬁes as a term or a colloca-tion. While term mining and collocation mining,as a whole, involve almost the same ... Proceedings of the 39th Annual Meeting of the Association for Com-putational Linguistics and the 10th Conference of the European Chapter of the Association for Com-putational Linguistics, pages ... general-language parsers (in the case of CE).Therefore, most recent approaches in both areashave backed off to more shallow linguistic ﬁlter-ing techniques, such as POS tagging and phrasechunking...

Báo cáo khoa học: Catalytic residues Lys197 and Arg199 ofBacillus subtilis phosphoribosyl diphosphate synthase Alanine-scanning mutagenesis of the flexible catalytic loop ppt

... 5¢-CGTCCGCGTGCAAACGTGG and 5¢-CCACGTTTGCACGCGGACG for P20 2A (pHO380), 5¢-CGCCGTCCGCGTCCAGCTGTGGCGGAAGTCATGAATATTGTAGGTAACATCGAAGGG and 5¢-CCCTTCGATGTTACCTACAATATTCATGACTTCCGCCACAGCTGGACGCGGACGGCG ... forN20 3A (pHO392), 5¢-CGCCGTCCGCGTCCAAACGCCGCGGAAGTCATGAATATTGTAGGTAACATCGAAGGGand 5¢-CCCTTCGATGTTACCTACAATATTCATGACTTCCGCGGCGTTTGGACGCGGACGGCG for V20 4A (pHO393), 5¢-CGTGGCGGCGGTCATGAATATTGTAGGand ... 5¢-CGTGGCGGCGGTCATGAATATTGTAGGand 5¢-CCTACAATATTCATGACCGCCGCCACG forE20 6A (pHO382), 5¢-CGTGGCGGAAGCGAGTAGG and5¢-CCTACAATATTCATCGCTTCCGCCACG for V20 7A (pHO383), and 5¢-CGTGGCGGAAGTCGCGAATATTGTAGG and 5¢-CCTACAATATTCGCGACTTCCGCCACGfor...

Báo cáo khoa học: Nucleotide binding to human UMP-CMP kinase using ﬂuorescent derivatives ) a screening based on afﬁnity for the UMP-CMP binding site potx

... FranceHuman UMP-CMP kinase (UCK) plays a key role in the ribonucleoside and deoxyribonucleoside salvagepathway and in the anabolic phosphorylation of nucleo-side analogs used as antiviral and anticancer ... NMP kinase [2] and ﬁnally NDP kinase [3] and ⁄ orone of the enzymes capable of synthesizing ATP, suchas phosphoglycerate kinase [4,5], pyruvate kinase orcreatine kinase [6]. However, acyclic ... 2 Van Rompay AR, Johansson M & Karlsson A (2000)Substrate speciﬁcity and phosphorylation of nucleosidesand nucleoside analogs by mammalian nucleosidemonophosphate kinases. Pharmacol Ther...

Báo cáo khoa học: Isoquinoline-1,3,4-trione and its derivatives attenuate b-amyloid-induced apoptosis of neuronal cells pdf

... caspasesand that the most promising caspase inhibitors tested in clinical trials are pan-caspase inhibitors, the abilityto inhibit most of the caspases is a desirable feature of isoquinoline-1,3,4-trione ... against Ab(25–35)-induced apoptosis by attenuating the activation of caspases and associated caspase cascades. Furtherstudy is in progress to verify their therapeutic effects in animal models of Alzheimer’s ... deter-mined by the Bradford method with BSA as the standard.Caspase-3 enzymatic assay and inhibition of catalytic activity The enzymatic activity of caspase-3 at 35 °C was deter-mined by measuring...

Báo cáo khoa học " ĐẶC ĐIỂM KỸ THUẬT TRONG THI CÔNG GIÀN MÁI KẾT CẤU THÉP KHẨU ĐỘ LỚN NHÀ THI ĐẤU THỂ DỤC THỂ THAO THÀNH PHỐ ĐÀ NẴNG " potx

... ph i hợp thử nghiệm nhiều lần và đã tìm ra được quy trình khắc ph c như sau: - Dùng giấy nhám thô v a ph i đánh sạch bề mặt bị châm kim; - Pha loãng sơn lớp 2, phun lớp mỏng lên bề mặt v a ... s a ch a các mặt bích hở 80mm ÷ 90mm là thay đoạn ống bị hụt bằng đoạn ống dài hơn (khi nối ống có lót ống bên trong). Giàn giáo ph c vụ s a ch a lắp dựng ở r a sàn thao tác. Theo đề xuất c a ... xảy ra bất kỳ sự cố nào), Kinh nghiệm rút ra là, các bên cần ph i thảo luận kỹ khi l a chọn biện ph p thi công vì biện ph p ph hợp làm cho công việc thi công ph c tạp trở nên đơn giản hơn, an...

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Báo cáo khoa học: Methylcitrate synthase from Aspergillus fumigatus Propionyl-CoA affects polyketide synthesis, growth and morphology of conidia ppt

... environment and may play a role in the resistance against killing by alveolar macro-phages [23,27,28]. In contrast to that the white conidia of a pksP mutant strain posses a plain surface andseem ... generally contain large amounts of proteins wecan assume that a germinated A. fumigatus spore willuse these proteins as carbon source. In that case, the amino acids isoleucine, valine and methionine ... activating enzymes are intact, acetate is always the preferred substrate over propionate [3]. In order to investigate the activation of acetate andpropionate in A. fumigatus, activities of the...

Tài liệu Báo cáo khoa học: Structure determination and biochemical studies on Bacillus stearothermophilus E53Q serine hydroxymethyltransferase and its complexes provide insights on function and enzyme memory doc

... by:FD¼ A TÀ A N =A DÀ A NWhere A Nis the absorbance of the protein sample at 30 °C, A Dis the absorbance of the sample at 80 °C and A Tis the absorbance of the protein sample at different ... cuvettes and equilibrated at 30 °C to obtain the baseline. The samples were heated from 30 °Cto90°Cat the rate of 1 °CÆmin)1using a programmable thermalcontrol unit. The absorbance change in each ... tetrahydrofolate.J Mol Biol 296, 155–168.12 Trivedi V, Gupta A, Jala VR, Saravanan P, Rao GS,Rao NA, Savithri HS & Subramanya HS (2002) Crystalstructure of binary and ternary complexes of...

Tài liệu Báo cáo khoa học: Nop53p, an essential nucleolar protein that interacts with Nop17p and Nip7p, is required for pre-rRNA processing in Saccharomyces cerevisiae pdf

... eukaryotic translation and mRNA stability. A short upstream open reading frame strongly inhibitstranslational initiation and greatly accelerates mRNAdegradation in the yeast Saccharomyces cerevisiae.J ... pre-rRNA processing in Saccharomyces cerevisiaeDaniela C. Granato1, Fernando A. Gonzales1, Juliana S. Luz1, Fla´via Cassiola2,Glaucia M. Machado-Santelli2and Carla C. Oliveira11 Department ... [3H]uracil labeling.An aliquot of 20 lg of total RNA was loaded in each lane. The ﬁgures show autoradiographs of RNA transferred to nylon membranes incuba-ted in En3Hance (Amersham Biosciences)....

Báo cáo khoa học: "Does Size Matter – How Much Data is Required to Train a REG Algorithm?" potx

... Domain complexity appears to be a factor in how much training data is needed: using Dice as anevaluation metric, training sets of 10 sufﬁced in the simple furniture domain, while in the more complex people ... REG challenges. For the sake of compari-son, we also follow the evaluation methodology of the REG challenges, training and testing on two do-mains (a furniture and a people domain), and usingtwo ... “semantically transparent” data sets of various sizes and evaluating on a held-out testset. The graph-based algorithm seems a good can-didate for this exercise, in view of its performance in the...

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