Báo cáo khoa học: Mutants of Saccharomyces cerevisiae deﬁcient in acyl-CoA synthetases secrete fatty acids due to interrupted fatty acid recycling docx

... Authors Journal compilation ª 2008 FEBS Mutants of Saccharomyces cerevisiae deﬁcient in acyl-CoA synthetases secrete fatty acids due to interrupted fatty acid recycling Michael Scharnewski1, Paweena ... certain pools of fatty acids. To assess the role of acyl-CoA synthetases in regu-lating particular pools of fatty acids, we used mutants of S. cerevisiae characterized by various combinations of ... exogenous fatty acids, additional release of internal fatty acids had to contribute to the increase of intracellular fatty acids. However, analysis of thepreviously mentioned fatty acid speciﬁcity of...

Báo cáo khoa học: Complementation of coenzyme Q-deﬁcient yeast by coenzyme Q analogues requires the isoprenoid side chain ppt

... Protein that interacts with silencing proteins at the telomere, involved in transcriptionalsilencing; has a role in localization of Bcy1p, a regulatory subunit of protein kinase A;implicated in ... respiratory chain complexes.The next possibility is that the protein of interest isinvolved in the transport of exogenous CoQ from theextracellular medium into the cell and on to mitochon-dria, ... ana-logues restoring respiration in isolated mitochondria.This suggests the presence of a protein that is able to recognize subtle differences in the side chain of CoQ.This protein is not part of the...

Báo cáo khoa học: Interaction of human steﬁn B in the preﬁbrillar oligomeric form with membranes Correlation with cellular toxicity doc

... 17.6Fig. 5. Insertion of stefins into DOPC and DOPG monolayers. (A)Kinetic traces of the insertion into DOPG lipid monolayers at initialpressure of 5 mNÆm)1. The proteins were injected into the ... forsome proteins that bind specifically to membranes,such as the small membrane-binding domains involved in cell signaling [39,40] or domains used by pore-form-ing toxins for attachment to the membranes ... directlylinked to the aggregation of mutant proteins. Proteinaggregates, in the form of amyloid plaques, neurofibril-lary tangles, intracytoplasmic or intranuclear inclusions[1] lead to increased...

Báo cáo khoa học: Mechanism of the ring contraction process in vitamin B12 biosynthesis by the anaerobe Propionibacterium shermanii under aerobic conditions docx

... ringcontraction involves the migration of ring A after for-mation of the d-lactone from the ring A acetate group to C20, following the methylation of Co-precorrin-3 atC17 [6,7]. In the aerobe Pseudomonas ... feeding experimentswith d-amino[1-13C]levulinic acid (a biosynthetic intermediate of tetra-pyrrole) and d-amino[1-13C,1,1,4-18O3]levulinic acid in combination with13C-NMR spectroscopy. ... led to the suggestion that ring contractioninvolved13C,18O-precorrin-4 formed from the migra-tion of ring D followed by cleavage of the carbon–oxy-gen bond of the c -lactone at C1 of 13C,18O-precorrin-3x...

Tài liệu Báo cáo khoa học: Roles of AP-2 transcription factors in the regulation of cartilage and skeletal development doc

... helix–span–helix domain at the C-terminus shown in blue medi-ates dimerization and, together with the basic domain, (red) DNA-binding. ‘*’, amino acids that are identical in all sequences in the align-ment; ... role of AP-2a and AP-2e in chondrogenesis. Overview of the differen-tiation stages during chondrogenesis andthe involvement of transcription factors(henatoxylin and eosin-stained section of ... over-lapping features, such as orofacial clefting and occa-sional lip pits, with Van der Woude syndrome, in which disruption of an AP-2a-binding site within aninterferon regulatory factor 6 enhancer...

Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx

... appears to besufficient to inhibit lysosomal degradation of modifiedproteins. Interestingly, apoB from oxidized LDL hasbeen shown to accumulate in secondary lysosomes in macrophages because of inefficient ... and either a mAb to CD36 [47], the SR-A inhib-itor fucoidin, or AGE–HSA, which is known to bind to RAGE [48]. The inhibition of uptake observed withthe mAb to CD36 or fucoidin indicates that ... time point (Fig. 2D).The extent of protein endocytosis, degradation andintracellular accumulation of apoB was increased in HMDMs exposed to glycolaldehyde-modified LDL in comparison to those...

Tài liệu Báo cáo khóa học: Suppression of b1,3galactosyltransferase b3Gal-T5 in cancer cells reduces sialyl-Lewis a and enhances poly N-acetyllactosamines and sialyl-Lewis x on O-glycans Lydia Mare and Marco Trinchera pdf

... modiﬁca-tions. Total lysates were obtained by boiling 10 min in phosphate-buffered saline containing 0.5% SDS and 1.0%2-mercaptoethanol, and spinning at 12 000 r.p.m. for10 min. The clean supernatants ... with a linearized plasmid containing a 553-bpfragment of b3Gal-T5 cDNA, that includes the initial360 bp of the coding sequence and 192 bp of the 5¢untranslated region of the gene, placed in the ... puriﬁcation of the included volume of the column in (A), largeO-glycans (j), obtained as the included volume of the column in (C),and small O-glycans (s), obtained as the included volume of the...

Tài liệu Báo cáo khoa học: "Integration of Large-Scale Linguistic Resources in a Natural Language Understanding System" pdf

... corresponding to nouns, because our system makes little use of hierarchical information about other parts of speech.' There are about 60,000 of these noun concepts in WordNet, including ancestor ... assumptions for all linguistic information, to be described later. There are four linguistic servers, corresponding to the four major categories of lexical information used in our system: lexicon, ... each individual sense, a process that we could not do automatically. 3 Default Linguistic Information If information about a word is not found in any of our linguistic resources, the NL Engine...

Tài liệu Báo cáo khoa học: Localization of N-linked carbohydrate chains in glycoprotein ZPA of the bovine egg zona pellucida pptx

... property of the N-linked chains of bovine zonaproteins is due to sialic acids [14], whereas many sulfates arelinked to the N-acetyllactosamine repeats of porcine zonaproteins [7,24]. The N-linked ... (sialidase digests of the acidicfraction in Table 1) were found in this analysis of ZPA.These sialic acids must be linked to the nonreducingterminal b-Gal residues of the core regions of the complex-type ... sites of bovine ZPA. The amino acid sequence of bovine ZPA, as deduced from cDNA and N-terminal amino acid sequence analyses [16,18]. Solid and dotted underlines indicate theN-terminal amino acid...

Báo cáo khoa học: Role of the C-terminal extension in a bacterial tyrosinase Michael Fairhead and Linda Thony-Meyer doc

... 37–357C-terminal extensionamino acids 358–518Copper binding motifArg40Phe453Cys84Tyr349Tyr347Copper binding motifPro-tyrosinase 481 amino acids Core domain 36–357C-terminal extensionamino ... Determination of the mass of the proteins byMS revealed masses of 36 506 kDa (recombinant coretyrosinase) and 37 874 kDa (trypsinized pro-tyrosi-nase) corresponding to a C-terminal amino acid of Val357 ... importance of a phenylalanine residue in this C-terminaldomain. These results demonstrate that the V. spinosum tyrosinase is a newexample of this interesting family of enzymes. In addition,...

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