... generation. Theresulting serum was immunoaffinity purified and tested for reaction against HydA1 and HydA2 overexpressed in E. coli and against HydA2 in anaerobically induced algalextracts.Algal ... byPCR in 50 lL reactions that contained 1 lLofKODHOTSTART polymerase (Novagen, Madison, WI, USA),25 lL of BAC clone cell-free lysate as template, 125 ngeach of forward (5¢-CTGGACGTGACAAACAAGACCC-3¢, ... days.cDNA library screening Two specific primers, BE5P1 (5¢-AACATCTTCAAGGAGCGTGGCATC-3¢)andBE3P1(5¢-AGACAGCAGGAGACTCACAATCAC-3¢), were used to amplify a C. rein-hardtii expressed sequence tag (EST),...