Báo cáo khoa học: Mapping of the 45M1 epitope to the C-terminal cysteine-rich part of the human MUC5AC mucin potx

Báo cáo khoa học: "Capturing Paradigmatic and Syntagmatic Lexical Relations: Towards Accurate Chinese Part-of-Speech Tagging" pdf

... features; the number is the total number of the clusters; the sym-bol “+” in the Data column means which portion of the Gigaword data is used to cluster words; the sym-bol “S” and “SS” in parentheses ... tagging accuracies relative to the length of the spans. We can see that with the increase of the number of words governed by the token, the difﬁculty of its POS prediction increase.This analysis ... range of conditions. This con-ﬁrms the importance of the paradigmatic relations.We then present a comparative study of our taggerand the Berkeley parser, and show that the combi-nation of the...

Báo cáo khoa học: A ‘pure’ chemoattractant formylpeptide analogue triggers a speciﬁc signalling pathway in human neutrophil chemotaxis potx

... Cell migration in the presence or absence of the chemotactic factor was evaluated by estimating the distance(in lm) migrated by the leading-front of the cell, after the method of Zigmond and Hirsch ... with other mechanisms [12,13], and may actat several steps in the cascade. MAPK phosphoryla-tions have an impact on cascade processes in the cyto-plasm, the nucleus, the cytoskeleton and the ... kinase 2 of pathway p38 andso dissociate from F-actin to allow cytoskeletal rear-rangement [34].Therefore, elucidation of the mechanism of inhibi-tion of neutrophil movement is of great importance...

Báo cáo khoa học: Mapping of the 45M1 epitope to the C-terminal cysteine-rich part of the human MUC5AC mucin potx

... MUC5AC, with epitopes locatedin the MUC5AC C-terminal cysteine-rich part. Further localization of the epitopes of 45M1, 2-12 M1 and 166M1In order to further locate the epitopes of the hithertounmapped ... in the N-terminal region of the C-terminal cysteine-rich part of MUC5AC, pre-sumably in the last CysD domain of the mucin. The epitope of 2-12M1 is located in the sequence corre-sponding to the ... Mapping of the 45M1, 166M1 and 2-12M1 epitopes on the C-terminal cysteine-rich part of human MUC5AC. The upper part of the ﬁgure shows a schematic representation of full-length human MUC5AC with...

Tài liệu Báo cáo khoa học: Mapping the functional domains of human transcobalamin using monoclonal antibodies pptx

... surface of holo-TC. Western blot analysis of the CNBr cleavage fragments of TC allowedus to distribute the epitopes between two regions, which spanned either the second quarter of the TC sequence ... assigned to the second quarter of TCsequence, whereas epitope clusters 1 and 3 (group 2)were localized to the last quarter of the full-lengthsequence.Antigenic properties of the synthetic peptidesTwo ... of TC (this study, [16]) supplemented the epitope mapping and provided a deeper insight intooperation of the TC domains. The binding of TCÆCbl to the receptor was suppressed by many antibodies,...

Tài liệu Báo cáo khoa học: Mapping of the epitope of a monoclonal antibody protecting plasminogen activator inhibitor-1 against inactivating agents pptx

... Localization of the amino acids in the epitope for Mab-1. The structure shown is a ribbon representation of the coordinates of Stoutet al. [40]. The side chains of the amino acids in the epitope and ... measurement of the increase in absorbance at 405 nm. The specific inhibitoryactivity of PAI-1 was calculated from the amount of PAI-1that had to be added to inhibit 50% of the uPA. The half-lives of the ... on the specific inhibitory activity of PAI-1 To measure the effects of antibodies and inactivators on the specific inhibitory activity of wt and substituted PAI-1, i.e. the fraction of inhibitor...

Tài liệu Báo cáo khoa học: Mapping the functional domain of the prion protein docx

... isoform of the prion protein in brains of individuals with these diseases.This abnormal form of the protein (PrPSc) is also suggested to be the infectious agent in the disease on the basis of infection ... PrPchave also beenshown to inﬂuence the level of the activity of the protein, asprotein with the sequence of the mouse ÔbÕ allele is moreCorrespondence to D. R. Brown, Department of Biology and Bio-chemistry, ... according to the epitope to which theybind. It can be clearly seen that the antibodies and antiserathat inhibit PrP’s activity are clustered around two parts of the protein. The ﬁrst cluster is in the...

Báo cáo khoa học: Mapping of the functional phosphate groups in the catalytic core of deoxyribozyme 10–23 potx

... P2, P4 and P9–13lowered the krelvalues by c. 50%. The replacement of 3mm Mg2+with 3 mm Mn2+resulted in a restoration of the activity to the level of the kMnobs of the unmodifiedenzyme (Table ... °C.ckMgrel¼ the ratio of the kobsvalues of modified deoxyribozyme to unmodified deoxyribozyme, in the presence of Mg2+.ekMnrel¼ the ratio of the kobsvalues of modified deoxyribozyme to unmodified ... coordi-nation of the metal cation to one of the two (the proRPor the proSP) oxygen atoms. However, it shouldbe noted that the kobsvalues were c. 30-fold higher in the presence of Mn2+compared...

Báo cáo khoa học: Mapping of the interaction site of CP12 with glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii Functional consequences for glyceraldehyde-3-phosphate dehydrogenase pot

... Wetherefore mutated Asp36 and Glu39 of CP12 into Alaor Lys. To test whether the interactions of thesemutants with the other partner, GAPDH were im-paired, we tried to reconstitute in vitro the ... of the C-terminus of CP12 to thioredoxin in the GAPDH ⁄ CP12 complex is speciﬁcally due to the inter-action of CP12 with GAPDH.Impact of cysteine residues of CP12 on the reconstitution of the ... BPGA. To date, mapping of the interaction site of CP12had not studied but our results give some clues withregard to the binding of CP12 to GAPDH. The impact of CP12 binding on the activity of GAPDH...

Báo cáo khoa học: Mapping of the active site of glutamate carboxypeptidase II by site-directed mutagenesis potx

... byintroducing the 3-carboxybenzyl group to the P1¢ sidechain of the inhibitor together with the sulfhydrylzinc-binding moiety [32]. To analyze the binding mode of the sub-strate ⁄ inhibitor to the active ... the binding of the glutarate part of the inhibitor in the S1¢ pocket contributes to the inhibition effect of the speciﬁc inhibitor 2-PMPA[29]. This notion can be supported by the fact that the R210A ... mutation Y552I leads to the decrease of both the Kmand kcatvalues. The mutation of Gly518 to Pro led to a slight increase of the Kmvalue and oneorder of magnitude decrease of the kcatvalue(Table...

Báo cáo khoa học: Mapping of chorismate mutase and prephenate dehydrogenase domains in the Escherichia coli T-protein doc

... from the product-binding pocket of the CM site to the substrate-binding pocket of the PDH site,then the weak CM activity of the CM fragments might bedue to slow product release or trapping of ... intoindividual domains of the T-protein. A further goal of the study was to ascertain whether feedback inhibition by Tyrmight also involve a discrete region of the T-protein. The established domain ... considered for the consistently low levels of mutase activity. The association of engineered fragments into homodimers, shown to beimportant in the monofunctional mutase derived from the E. coli...

Xem thêm