... pump and
in vitro vacuolar acidification in hepatoma HepG2 cells. The vacuolar H
+
-
ATPase inhibitor bafilomycin and the cathepsin D inhibitor pepstatin A
partially inhibited, both in vivo and in ... in NaCl ⁄ P
i
for 4 min and
0.5% saponine in NaCl ⁄ P
i
for 10 min. Permeabilized cells
were blocked for 20 min with 10% horse serum in NaCl ⁄ P
i
,
and then incubated fo...
... binding of ACP to
the docking site followed by a conformational change in
ACP proposed to inject the acyl chain into the sub-
strate-binding pocket [32]. If a similar mechanism is
involved in ... protein was crystallized in
hanging drop experiments at 2 94 K with reservoirs contain-
ing 2% (w ⁄ v) poly(ethylene glycol) 40 0, 100 mm bis-Tris
propane (pH 6.5) and 1.9 m (NH
4
)
2
SO...
... in the initial hour,
which could be due to prior attainment of an active
conformation by the enzyme, remaining stable up to
240 min (4 h) after which there was a decline (Fig. 4C,D),
indicating ... radiation at 1. 541 4 nm (Enraf Nonius Co., Bohemin,
NY). The relative intensity was recorded in a scattering
range (2h)of0 45 °. The crystallinity index (CrI, %) was
determined using the for...
... be used in the construction.
That is, beginning with only characters in the lexi-
con and using the training data to alter the current
lexicon in each iteration. This is also an interesting
direction.
References
Maximilian ... Brill, and Andreas Stolcke. 2000.
Finding consensus in speech recognition: Word er-
ror minimization and other applications of confusion
networks. Comp. S...
... UIM-mediated ubiquitin signaling in cells, we
performed a proteomic screen for Hrs UIM-interacting ubiquitinated pro-
teins in human brain by using an in vitro expression cloning screening
approach. ... Proteins can be either monoubiquitinated
or polyubiquitinated by attachment of a multiubiquitin
chain linked through one of the internal lysine residues
in ubiquitin [2]. K48-linked po...
... documents in order to include the
logical lead in most instances. Using a fixed
amount of leading text regardless of document
length would have resulted in LEADs that include
13 64
too much ... queries to the headline and leading text than if
they were to apply their queries to the full text.
Documents in our news database have several text
fields including HEADLINE and B...
... nascent chains that can affect protein elongation in
prokaryotes and in mammalian cells [67–71], and act as a
discriminating gate. Striking examples are SecM, a protein
belonging to the secretion ... cross-linking data [44 ] and
rationalizing the difficulties of its localization [18,23 ,44 ,45 ].
In accordance with the finding that despite sparsomycin
universality, ribosomes from...
... fluoro-
graphy.
Transient transfection of WT and mutant CYP2D6
in COS-7 cells
COS-7 cells were cultured in DMEM containing 10% fetal
bovine serum and gentamycin (50 lgÆmL
)1
). Cells were
transiently ... in
CYP2D6 content, ranging from a relatively high level
in HL1 34 and 137 to a moderate level in HL136, low
levels in HL132, 139 and 140 , and almost undetectable
leve...