Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt
Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt
... FEBS Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) Juliana H. C. Smetana and Nilson I. T. ZanchinCenter for ... the interaction of TIPRL with the C-terminal region of the catalytic subunits of type 2A phosphatases. TIPRL forms a heterotrimeric complexwith PP2Ac and a4 and does not compete with a4 forPP2Ac ... BrazilType 2A phosphatases are part of the PPP subfamilythat is formed by PP2A, PP4 and PP6, the mam-malian orthologs of yeast Pph21 ⁄ 22, Pph3 and Sit4,respectively. These are serine ⁄ threonine phosphataseswith...
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Báo cáo khoa học: Molecular analysis of the interaction between cardosin A and phospholipase Da Identification of RGD/KGE sequences as binding motifs for C2 domains pdf
... illustrated by the superimposition of the 3D structures of kistrin[32] and cardosin A (Fig. 8). The importance of bothmotifs and in particular their basic residues was fur-ther emphasized by the complete ... at the tip of a loop protruding away from the core of the protein. This amino-acid motif mimics RGD in terms of charge and is positioned at the tip of a loop and istherefore reminiscent of RGD ... [12,14,15,19,20,22],there are no reports on the true nature of the RGD-containing proteins and their interacting partners. The involvement of the PLDa C2 domain in theseFig. 7. C2 domain is degraded by cardosin...
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
... gifts of the MAP2 cDNA and the pCAG expression vector,respectively. The present study was supported by grants from the Japanese Ministry of Education,Culture, Sports, Science and Technology; the ... 1657Discussion The present study revealed the structural and func-tional determinants of the protein–protein interaction between v-KIND and MAP2 (i.e. the MAP2-bindingcore module in v-KIND and the v-KIND-binding ... phosphorylation of MAP2 by JNK1(such as downstream of v-KIND overexpression [12])enhances the activity of MAP2 to bind to microtubules and promote their assembly [18], and that the inhibition of JNK1...
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Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc
... depends on the relative amplitude and rate constant of the J–Iphase compared to the amplitudes and rate constants of the two other phases. The above should be gov-erned by the balance between the ... modeled by the superposition of the exponential rise to analyzequantitatively the contribution of each phase. The amplitude and rate constant of each of the threephases can be calculated by deconvolution ... amplitude and half-time of the J–I phase were bothdecreased with DCMU, demonstrating that the J–I riseis not directly related to the reduction of the PQ pool.A further analysis of the influence of...
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf
... (Met1)-ONC(M23L) by the addition of AAP, as described in the Materials and methods. Completion of the hydrolysis wasconfirmed by the disappearance of the (Met1)-ONC(M23L) signal in the MALDI-TOF mass ... analyzed by MALDI-TOF MS. The addition of the protease is considered the zero time point. The relative intensities of the peaks from the mass spectrawere used to calculate the fraction of Pyr-ONC ... diminished the Tm10;11 by 6 °CandtheDGU10;11 by 3.5 kJÆmol)1, whereas substitution of methionine withleucine at position 23 diminished the midpoint of the thermal denaturation curve (Tm )by7 °Candthefreeenergy...
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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt
... DEC1 with BMAL1 and the bin ding to the E-box.Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box.However, substitution of His57 for ... regulating the mammalian circadian rhythm by suppressing the CLOCK/BMAL1-activated promoter. The interaction of DEC1 with BMAL1 has been suggested a s one of the molecular mechanisms of the suppression ... increase in the mutantmice, the disruption of DEC1 might be compensated by DEC2. Further investigation is required to clarify the functions of DEC1 and DEC2. The suppressive activities of DEC1 and DEC2...
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Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc
... show that the data fit well with the indi-vidual determination of flux control coefficients by PFK, PK and LDH. The role of the las operon is dis-cussed on the basis of the distribution of flux controlfor ... individually and simultaneous by changing the expression of the respective genes. We found that nei-ther the individual enzymes nor the sum of the lasenzymes had significant control on the glycolytic ... the activity of the PTS system and therebyresult in further increases in hexose phosphate pools. The decreased LDH activity may contribute further tothis effect by causing an accumulation of...
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Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf
... bond, linkage of the activesite Ser of the protease to the carboxyl group of P1 by anester bond and insertion of the N-terminal part of the RCLas strand 4 in b-sheet A (s4A) of the serpin. ... behaviour of the serpin.Complex formation between serpins and their cognateproteases is fuelled by the thermodynamic properties of the serpin. Accordingly, insertion of the RCL as s4A and the ensuing ... 5), and the salt-bridgebetween E132 and R135 [18] contribute to the thermo-dynamic stability of the stressed conformation and/ orare important for the positioning of hF in a waydelaying the...
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Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc
... appearance of an additional peak at 7.65 mLon the elution profile. Increase of the temperature of incubation was accompanied by the simultaneous increase of the peak eluted at 7.65 mL and decrease of the ... 0.05, and 0.15 ± 0.06 lMin the absence of HSP25, and in the presence of the wild type HSP25 and its 3Dmutant, respectively. At the same time, both wild type and especially 3D mutant of HSP25 ... of the time of heating at 43 °C leading todecrease of parameter A up to 2.2–2.3 was accompanied by further decrease of the initial rate of polymerization. Whenparameter A was in the range of...
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Tài liệu Báo cáo khoa học: Fluorescence analysis of the Hansenula polymorpha peroxisomal targeting signal-1 receptor, Pex5p pdf
... spectroscopy. The intensity of Trp fluorescence of purified HpPex5p increased by 25%upon shifting the pH from pH 6.0 to pH 7.2. Together with the results of fluorescence quenching by acrylamide, thesedata ... calculation of the fraction (fa) and the effectivequenching constant (KQ) for the most accessible class of Trp chromophores [16]. The pH dependence of the Trp fluorescence was studiedin the pH ... 348.8 nm. Analysis of the fluorescence intensity decay and anisotropy decay wasperformed with a model of discrete exponentials using the TRFA Data Processing Package of the Scientific SoftwareTechnologies...
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