Báo cáo khoa học: Unraveling multistate unfolding of pig kidney fructose-1,6bisphosphatase using single tryptophan mutants docx

... The Authors Journal compilation ª 2007 FEBS 5349 Unraveling multistate unfolding of pig kidney fructose-1,6-bisphosphatase using single tryptophan mutants Heide C. Ludwig, Fabian N. Pardo*, Joel ... activity of the nonrecombinant, recombinant wild-type and themutant pig kidney FBPases as a function of GdmClTable 1. Kinetic parameters for wild-type and single tryptophan mutants of pig kidney ... theenzyme isolated from pig kidney and 0.904 for the single- tryptophan mutants (determined by comparison with theenzyme isolated from pig kidney) .Spectrophotometric assay of fructose-1,6-bisphosphatase...

Tài liệu Báo cáo khoa học: "Lexicalized Stochastic Modeling of Constraint-Based Grammars using Log-Linear Measures and EM Training" ppt

... y1 w3 h3" alt="" 687072747678808284868810 20 30 40 50 60 70 80 90precisionnumber of iterationscomplete-data estimationincomplete-data estimation...

Tài liệu Báo cáo khoa học: "DiMLex: A lexicon of discourse markers for text generation and understanding" docx

... group of words, many of which are traditionally treated as function words belonging to the realm of grammar rather than to the lexicon. But for a single discourse relation there is often a ... variety of semantic and pragmatic overtones, which render the choice of a marker meaning- driven, as opposed to a mere consequence of structural decisions. Furthermore, a number of lexical ... detailed studies of individual groups of markers, such as [Vander Linden, Martin 1995] for PURPOSE markers. Besides, the Linguistics literature offers fine-grained analyses of individual markers,...

Báo cáo khoa học: "Efﬁcient Inference of CRFs for Large-Scale Natural Language Data" docx

... {yt}Tt=1are sequences of in-put and output variables. Respectively, where T is thelength of sequence, xt∈ X and yt∈ Y where X is theﬁnite set of the input observations and Y is that of theoutput ... amethod of choosing appropriate values for the settingfunction δ of the active set and for the constant valueω of the inactive set. These two problems are closelyrelated. The size of the active ... becauseour goal is to evaluate the efﬁciency of inference algo-rithms. Moreover, using the previous state -of- the-artfeatures we expect the achievement of better accuracy.All our models were trained...

Báo cáo khoa học: "Finite-state Approximation of Constraint-based Grammars using Left-corner Grammar Transforms" doc

... of a top-down parser just before the construction of X consists of (the label of) X followed by the sequence of labels on the right siblings of the nodes encountered on the path from X back ... the kind of parser we want to study using G. 1.2 Mappings from trees to trees The transformations presented here can also be un- derstood as isomorphisms from the set of parse trees of the ... a top-down parse using the transformed grammar is isomorphic to some other kind of parse using the original grammar. Thus grammar transforms provide a simple, compact way of describing various...

Báo cáo khoa học: " Improved Automatic Detection of Zero Subjects and Impersonal Constructions in Spanish" docx

... precision of 74% and recall of 60%, and K-nearest neighbors in TiMBL: bothin (Evans, 2001) with precision of 73% and recall of 69%, and in (Boyd et al., 2005) with precision of 82% and recall of 71%.Since ... learning curve of Figure 1 (left) presents theincrease of the performance obtained by Elliphant using the training data randomly ordered. Theperformance reaches its plateau using 90% of thetraining ... number of instances of each class (right) for a given percentage of training data.annotated instances (fewer than 1,500) to achievegood results.6.3 Impact of GenreTo examine the inﬂuence of the...

Báo cáo khoa học: "A Grammatical Approach to Understanding Textual Tables using Two-Dimensional SCFGs" docx

... regarded as a somehow “de-generate” form of text, unworthy of the same de-gree of attention as the rest of the text. But aswe will discuss, the degree of ambiguity in ta-ble understanding is ... patterns of logical relations we havefound in the course of empirically analyzing tablesfrom corpora. One advantage of this inventory of data models arises from our appropriation of re-lational ... Dayal(1997)), consists of a set of numeric measures(though in fact the data need not be numeric), each of which is determined by a set of dimensions.Each dimension is described by a set of attributes.For...

Tài liệu Báo cáo khoa học: Unraveling the catalytic mechanism of lactoperoxidase and myeloperoxidase A reﬂection on some controversial features Elena Ghibaudi and Enzo Laurenti docx

... integrated vision of the catalyticmechanism of both enzymes. The main points are: (a) are-evaluation of the role of superoxide as a reductant in thecatalytic cycle; (b) the existence of Cpd I isomers; ... addressing each of these questions, we will proposea new hypothesis that describes an integrated vision of thecatalytic mechanism of MPO and LPO.A brief survey of the absorption features of MPO, LPO ... other hand, this factdoes not represent proof of the presence of one or twooxidizing equivalents on the decay product of Cpd I.The EPR spectrum of a spin-trapped radical in MPOCpd I has been...

Báo cáo khoa học: Multiple-probe analysis of folding and unfolding pathways of human serum albumin docx

... concentrations of GdnHCl (0.25–7M)at 25 °C for 1 h. The emission spectra were collected overthe range of 310–400 nm using 295 nm as an excitationwavelength. Unfolding of HSA in the presence of bilirubinThe ... slope of the transition, DGUÀFH2Ois the freeenergy of unfolding in the absence of denaturant. Thefluorescence intensity (F) of a protein at equilibrium withdifferentconcentrations ofdenaturant[D]canbeexpressed ... this equation using the linear least-squares method to obtain the best-fitted values of m and Dm. The curve fittings were performed using MICROSOFT ORIGINsoftware.Calculation of the rate constants...

Báo cáo khoa học: Probing the unfolding region of ribonuclease A by site-directed mutagenesis potx

... rate constants of this RNase A variant correspond to those of wild-typeRNase A (Fig. 4), indicating that the local unfolding of theAla20 loop is independent of the global unfolding of theRNase ... also used to determine the unfolding rate constants of RNase A. Thus, both the local unfolding of this loop (vi a the cleavage a t Ala20–Leu21) and t heglobal unfolding of the RNase A molecule ... the concept of t he unfolding region.AcknowledgementsWe are grateful to Professor R. T. Raines (University of Wisconsin,Madison, WI, USA) for the gift of the plasmid pBXR, to ProfessorG. Vriend...

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