Báo cáo khoa học: Interference with the citrulline-based nitric oxide synthase assay by argininosuccinate lyase activity in Arabidopsis extracts docx

... Interference with the citrulline-based nitric oxide synthase assay by argininosuccinate lyase activity in Arabidopsis extracts Rudolf Tischner1,*, Mary Galli2,*, ... determine the source of arginine-dependent NOS activity in plants,using crude protein extracts from Arabidopsis leaves. By employing the citrulline-based NOS assay, an argi-nine-dependent activity ... converted to arginineand fumarate by argininosuccinate lyase (ASL;EC 4.3.2.1; Fig. 5). Argininosuccinate is normallymade from citrulline and aspartate by argininosuccin-ate synthetase, but it...

Báo cáo khoa học: Binding and activation of nitric oxide synthase isozymes by calmodulin EF hand pairs potx

... with strongbinding to the NOS CaM binding domains (Fig. 3).All other mutant CaM proteins failed to either activateor bind the cNOS enzymes. These results indicate that the N-terminal domain ... enzymes during binding and activation[4,5]. The present investigation, designed to furtherassess the role of the two CaM EF hand pairs in the binding and activation of nitric oxide synthase (NOS;EC ... binding domain with the oxygenase domain.If we initially assume that CaM binds to the NOSenzymes in the classical closed conﬁguration, the N-terminal EF hands bind the C-terminus of the targetpeptide...

Tài liệu Báo cáo khoa học: Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells pdf

... enrichment in iNOS activity within the particulate fraction of epithelial cells.Apical distribution of iNOS in intestinal epithelialcellsTo get more insight into the localization of iNOS in intestinal ... of iNOS to apical domains of intestinal epithe-lial cells.Particulate fraction association of iNOS in vivo In order to determine the distribution of iNOS in intestinal epithelial cells in vivo, ... the C-termi-nus of iNOS promotes in vitro interactions with the PDZ protein EBP50 [13]. Interestingly, EBP50 has dif-ferent binding partners including ezrin that can beanchored to the actin...

Báo cáo khoa học: "deals with the interpretation of conceptual operations" docx

... together with the name, the two extreme points of the chain leaving the intermediate undefined. The entire process, using generic knowledge as the reference point, is shown in Figure 2. Figure ... of the objects while the A-Box contains the set of the assertions on the objects. The former corresponds to the ability of describing by the use of NPs, and the latter to that of constructing ... one another. This makes It possible to insert into the language functions based on different processes. Taking for instance the history of the objects as a reference point, the objects themselves...

Báo cáo khoa học: "DEALING WITH THE NOTION "OBLIGATORY" IN SYNTACTIC ANALYSIS" docx

... (i.e. the surface form of valency). In this way, the approach is similar to other theories: In the lexical entries of the wordforms there is a pointer to the rules which can be applied with the ... darin, (The nature consists in ) Here in (I) and (3) the subject is obligatory, but in (2) only the direct object. In the other cases the prepositional objects are obligatory, thus the ... be sub- ordinated to nouns and verbs: many of them are optional, some of them stand in certain relations to others, etc. Thus we concentrate all these bundles by defining another form of...

Tài liệu Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves doc

... serine and gly-cine was reduced from 33% in the light to 25% in the dark (Fig. 7A). Glutamine and asparagine were the major amino acids in the xylem sap in both the light(63% of the total amino ... orglutamine was omitted from the reaction mixture. The kinetics ofFd-GOGAT activity were assayed by increasing the concentrationsof Fd isoprotein as indicated in the ﬁgure (C). Photosynthetic ... described in [17]. Free ammonium contents weredetermined by the phenol hypochlorite assay (Berthelot assay) . Soluble protein contents were determined by the Coomassie blue dye-binding assay (Bio-Rad...

Báo cáo khoa học: Expression of the Drosophila melanogaster ATP synthase a subunit gene is regulated by a transcriptional element containing GAF and Adf-1 binding sites pptx

... igestion with SalI (which recognizes the sequence underlined in the oligonucleotides), the DNAfragment was cloned into the pXp2 vector that contains the luciferase reporter g ene. The construct with ... both in vitro and in vivo [38].To analyse the involvement of the potential GAF andAdf-1 binding sites in activating the a-F1-ATPasepromoter, we eliminated the target sequences by site-directed ... fragment with reference to the transcription start point as established in this study. The relative promoter activities of the constructs measured in the luciferase assay are indicatedon the right by...

Báo cáo khoa học: Structure of the core oligosaccharide of a rough-type lipopolysaccharide of Pseudomonas syringae pv. phaseolicola docx

... glycoformsdiffering in t he postion of a terminal Rha residue occurs in the P. aeruginosa LPS [30], one of them being markedlysimilar to the Rha-containing glycoform o f the P. syringaeLPS core. ... species, that ofP. syringae being distinguished by the simultaneous pres-ence of GlcNAc and Rha. The same LPS core compositionwas revealed by other studies in all P. syringae strains t ested[11,13–16], ... performed by the proce-dure of Ciucanu and Kerek [20] with CH3I(0.3mL )in dimethylsulfoxide (0.5 mL) in the p resence of solid NaOH(stirring for 20 min before and 2 h after a dding CH3I), the reaction...

Báo cáo khoa học: Insights into the activation of brain serine racemase by the multi-PDZ domain glutamate receptor interacting protein, divalent cations and ATP ppt

... thatGRIP binding to brain SR induces a conformationalchange that increases the racemase activity by overtwo-fold. Although the PDZ6 domain of GRIP isinvolved in the interaction with the last ... at increasingsubstrate concentrations. The Lineweaver–Burk plot is depicted in the insert. These assays were performed in the presence of 1 mMCa2+and in the absence of ATP. (D) Increase in ... of the naturally occurring amino a cid l-serine into its race-mic counterpart d-serine [1,2]. In brain tissues,d-serine occupies the so-called ‘glycine site’ withi n the NMDA receptor together...

Tài liệu Báo cáo khoa học: Efﬁcient killing of SW480 colon carcinoma cells by a signal transducer and activator of transcription (STAT) 3 hairpin decoy oligodeoxynucleotide – interference with interferon-c-STAT1-mediated killing pdf

... andindicating that, in itself, the interaction of ODN with STAT3 induced these effects (i.e. inhibition of the cyclin-D1-dependent promoter, colocalization with STAT3 and STAT3 binding in pull-down ... (particularly in whole ani-mals) by impairing the action of STAT1-dependentinterferon. The results obtained in the present study, including the ability of the hairpin decoy STAT3 ODN to inhibitboth ... keycomponents of the STAT3 protein complex, therebyimpairing normal interaction with the nuclear trans-port machinery. Alternatively, the hairpin ODN itselfmight interact with components of the nuclear...

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