Báo cáo khoa học: Puriﬁcation and characterization of zebraﬁsh hatching enzyme – an evolutionary aspect of the mechanism of egg envelope digestion pot

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc

... composed of two each of the a and b subunits.Analysis of the N-terminal transit peptide of CLGATAnalysis of the CLGAT cDNA predicted a chloroplasttransit peptide upstream from the N-terminus of CLGAT. ... plants. Images 1 and 2 representphotographs of wild watermelon plants watered daily and subjec-ted to drought for 5 days, respectively, and images 3 and 4 showtheir thermal distributions analysed ... edn, pp. 32 1–4 48. Springer-Verlag,New York.28 Marc F, Weigel P, Legrain C, Almeras Y, Santrot M,Glansdorff N & Sakanyan V (2000) Characterization and kinetic mechanism of mono- and bifunctionalornithine...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... – – 16Dog – – 4Donkey – – 4Human A – 0 0Human B – 0 2Human O – 0 0Cow – 0 0Goat – 0 0Sheep – 0 0aData from [30] and [63].Table 3. The eﬀect of enzyme treatment of rabbit and horse erythrocytes ... surface. They are found at the outermostends of the sugar chain of animal glycoconjugate. They actas an important component of the ligands recognized by the lectins. Recognition can be affected ... haemagglutination.On the other hand, human blood cells A, B and O [44,45], and sheep have surface glycoconjugates rich in NeuAc [44] and thereby the lectin showed poor binding afﬁnity towardsthese erythrocytes.The...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulﬁde reductase from methanogenic archaea pdf

... (Hdr) is a key enzyme in the energy metabolism of methanogenic archaea. In the finalstep of methanogenesis, the mixed disulfide of the metha-nogenic thiol coenzymes coenzyme M and coenzyme B isgenerated ... of the genes encoding the subunits of the Hme complex and sequence analysis The N-terminal sequences of the four polypeptides presentin the purified enzyme preparation were determined byEdman ... oxidation of the heme groups of the enzyme byvarious compounds was tested to identify the physiologicalelectron acceptor of the enzyme. The enzyme was notoxidized by the heterodisulfide of coenzyme...

Báo cáo khoa học: Puriﬁcation and characterization of zebraﬁsh hatching enzyme – an evolutionary aspect of the mechanism of egg envelope digestion pot

... that of medaka hatching enzyme. Finally, the evolution of the mechanism of egg envelope diges-tion is discussed on the basis of the manner of the reciprocal or cross-species egg envelope digestion using ... swells the envelope. The cross-species digestion using enzymes and substrates of zebraﬁsh and medaka revealed that both ZHE1 and MHCE cleaved the same sites of the egg envelope proteins of two ... regions of egg envelope subunits wheremost of cross-links are located, and swelled the egg envelope. Therefore, the manner of egg envelope diges-tion is analogous between ZHE1 and MHCE. The cross-species...

Báo cáo khoa học: Puriﬁcation and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt

... cm). The column was run in20 mm 1,3-diamino propane, pH 11. Ion-exchange chroma-tography of two pools (A and B) that contained proteinbands of the expected mass range was then repeated on the Mono ... Biosystems).AcknowledgementsFinancial support for this research was provided by agrant to T. Kyndt from the ‘Institute for the Promo-tion of Innovation through Science and Technology inFlanders (IWT-Vlaanderen)’ and ... content in their latex; and (2) the pres-ence of other cysteine proteinases or isoforms in the latex. To investigate these two hypotheses, the proteinconcentration per milligram of dried latex,...

Báo cáo khoa học: Puriﬁcation and characterization of cathepsin B-like cysteine protease from cotyledons of daikon radish, Raphanus sativus docx

... electrophoresis and N-terminal sequence of the purified enzyme. (A) Native PAGE of the purified enzyme (0.5 lg) in the absence of SDS. (B) SDS ⁄ PAGE of the purified enzyme (2 lg) and N-terminal sequence of bands ... (Cys14–Cys43, Cys2 6– Cys71, Cys62–Cys128, Cys63–Cys67, Cys100–Cys132 and Cys108–Cys119 in human mature cathepsin B).Residues in the S1, S2 and S1¢ sites (S1, Gln23 and Gly74; S2, Glu245 and Gly198; ... correspondingto the N-terminal sequence (LPKSFDART) of purified enzyme and a conserved sequence (AGLPSSKN) in the C-terminal region of the catalytic domain of plantCBCP. The sequences of the sense and antisense...

Báo cáo khoa học: Puriﬁcation and characterization of Helicobacter pylori arginase, RocF: unique features among the arginase superfamily doc

... Department of Microbiology and Immunology and the College of Medicine, by Public Health Service grant CA101931 (toD.J.M.) from the National Institutes of Health, and the AustralianResearch ... concentration of 0.2 0–0 .25 mM), 5 0–1 00 pmol of each primer, and 2.5 U of thermostable DNA polymerase. E. coli was transformedby the calcium chloride method.Construction of the arginase mutant of H. ... After 1 h the reaction was stopped by the addition of 750 lL of acetic acid, and the color developedby the addition of 250 lL of ninhydrin (4 mgÆmL)1)at95 °C for 1 h. A standard curve of different...

Báo cáo khoa học: Puriﬁcation and characterization of NTPDase1 (ecto-apyrase) and NTPDase2 (ecto-ATPase) from porcine brain cortex synaptosomes doc

... Cu2+ and Ba2+were strong inhibitors of the analysed enzymes,whereas the presence of monovalent ions in the incubationmixture did not affect the rate of ATP and ADP hydrolysis(Table 4).ATP and ... ATP and ADP and the product of ATP and ADP hydrolysis is AMP – the compound with a very low affinity for P2 receptors. The above results confirm the participation of NTPDase1 in the scavenging of ... changed their affinitytowards ATP and ADP [1 1–1 5].NTPDase1 differs from NTPDase2 in respect to reactionproducts of ATP hydrolysis and in the ratio of the rate of ATP hydrolysis to the rate of...

Báo cáo khoa học: Puriﬁcation and characterization of three isoforms of chrysophsin, a novel antimicrobial peptide in the gills of the red sea bream, Chrysophrys major doc

... 5–5 5 min2 0–4 7% solvent B, and 5 5–8 0 min 4 7–1 00% solvent B. The efﬂuents were separated into two directions, and the ﬂowrate of one direction was adjusted to 0.6 mLÆmin)1 and that of the other ... hemolytic than melittin and morehemolytic than magainin (Fig. 10).Fig. 5. Cation-exchange HPLC of native P1, P2-2 and P3, and their synthetic peptides. Mixtures of synthetic P1-COOH and P1-CONH2(A), ... antibody (A, C, E and F) and with hematoxylin and eosin (D, F and H) as described in Materials and Methods.Immunopositive cells were mainly observed among connective tissuesat the base of the...

Báo cáo khoa học: Puriﬁcation and characterization of novel salt-active acharan sulfate lyase from Bacteroides stercoris HJ-15 ppt

... heparan sulfate. The heparin lyases have becomeincreasingly important in understanding the biological roles and structure of the glycoaminoglycans (and proteoglycan),which are involved in the ... involved in the well known anticoagulant activity[7] and the regulation of various cellular processes such as the potentiation of angiogenesis [8] and the modulation of cellular proliferation ... Fr-a, Fr-b and Fr-c) containing the activity of these enzymes werecollected separately and assayed for the activities degradingacharan sulfate and heparan sulfate. Fr-s had acharansulfate...

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