Báo cáo khoa học: Different roles of two c-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii Remodelling of interaction surfaces may enhance microtubule nucleation at low temperature doc

... FEBS Different roles of two c-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii Remodelling of interaction surfaces may enhance microtubule nucleation at low temperature Francesca ... transition state in cold thermal regimes.How do the unique features of the E. focardii c-tubu-lins enhance microtubule nucleation by the c-TuRCcomplex at low temperature, and what do these changesimply ... in the nucleation of cellular microtubules. In the present study, we report the characterization of c-tubulin from the psychrophilic Antarctic ciliate Eupl-otes focardii. In this organism, c-tubulin...

Tài liệu Báo cáo khoa học: Different modes of dipeptidyl peptidase IV (CD26) inhibition by oligopeptides derived from the N-terminus of HIV-1 Tat indicate at least two inhibitor binding sites doc

... Ile8 of the peptide interacting with the Tyr752 hydroxyl group of the enzyme. A hydrophobicinteractionofthesidechainofMet1ofTat(1–9)withthephenyl ring of the substrate Ala-Pro-pNA resulted in ... considerable stabilization of the complex. The interactions of the C-terminus as well as the N-terminus of Tat(1–9)-related peptides permitted the dock-ing of the inhibitor close to the active site ... resulted in aﬁxation of the aromatic leaving group.Rather similar interactions were obtained for docking of Trp2-Tat(1–9) to DP IV in the presence of the substrateAla-Pro-pNA. The salt bridges...

Tài liệu Báo cáo khoa học: Critical roles of Asp40 at the haem proximal side of haem-regulated phosphodiesterase from Escherichia coli in redox potential, auto-oxidation and catalytic control doc

... switchinginduces conformational changes in the FG loop regionand movement of t wo subunits. These structural changes may play critical roles in c atalytic regulation of the PD Edomain [4].Structures ... potential of the haem, rate constants o f CO, O2and CN b inding tohaem, and CD spectra in the Soret region [15–18],suggesting that this residue is critical for maintaining the electronic states of ... reduction of the haem b y sodium dithionite, excess dithionite was removed in the glove box by using a Sephadex G25 column. Toensure that the temperature of the solution was maintained at 25 °C, the...

Tài liệu Báo cáo khoa học: "Word Vectors and Two Kinds of Similarity" pptx

... spaces.Another interesting finding is that there was noclear peak in the graphs of Figure1. For SPI testitems, correct rates of the three methods increasedlinearly as the number of dimensions increased,r ... dfjdenotes the number of words whose sensedefinitions contain the word tj. The second term in parentheses in Equation (4) means the squareroot of the number of times the word tjoccurs in a collection ... collection of sense definitions for any words thatare included in the sense definitions of the word ti,while the third term means the number of times tioccurs in the sense definitions of tj. The...

Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx

... reinforce the argument that the basic domain should be exposed at the surface of the Tat-containing recombinant proteins and call other reasonsto explain the differences in the mechanism of internaliza-tion ... sunder evaluation.Comparative FACS analysis of the internalization of the full-length Tat protein construct and the Tat CPPDifferences in the mechanisms of internalization between the Tat peptide ... A), the internalization of the Tat±GFP fusion construct was not signi®cantly reduced in the presence of the excess of t he Tat peptide, in keep ing withseparate internalization pathways. Internalization...

Báo cáo khoa học: Respective roles of the catalytic domains and C-terminal tail peptides in the oligomerization and secretory trafﬁcking of human acetylcholinesterase and butyrylcholinesterase potx

... counterparts, in agreement with the higher mass of BChE subunits [37]. This indicates apredominant in uence of the catalytic domain onoligomerization.Role of the C-terminal cysteineMutation of the ... modiﬁed,suggesting that degradation was increased by suppres-sion of the cysteine.Thus, it appears that the effect of a C-terminalcysteine on the trafﬁcking of cholinesterase in the secretory pathway ... aggregationand increases the neurotoxicity of the Ab peptide in vitro, suggesting that it may participate in the patho-genesis of the disease [51,52]; this appears to depend oninteractions of...

Tài liệu Báo cáo khoa học: Mitogen-activated protein kinase phosphatase-1 modulated JNK activation is critical for apoptosis induced by inhibitor of epidermal growth factor receptor-tyrosine kinase pdf

... was recently examined for mutations, and clus-tering of mutations was detected in the part of the gene encoding the ATP-binding pocket. Screening forsuch mutations may identify patients who will ... activation of caspase 3 in human non-small cell lung cancer cell line PC-9. To investigate the signaling routeby which AG1478 induced apoptosis, we examined the activation of c-JunN-terminal kinase ... signal-ing pathways such as the Ras-MAPK pathway andPtdIns3K ⁄ Akt pathway, as a consequence of inactiva-tion of EGFR [10–13]. The PtdIns3K ⁄ Akt pathway isdownregulated in response to geﬁtinib...

Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc

... analysis, and the determination of released ammonia. The coupled enzymeassay revealed the mechanism of the deamination reactionand the subsequent metabolism, including the deaminationstep. The product ... prepared by incubating catechol with restingcells of a mutant, strain Y-2, of the aniline-assimilatingPseudomonas sp. strain AW-2 [20].ResultsSpectral changes during metabolism of 4-amino-3-hydroxybenzoic ... toidentify the cofactor of the deaminase from strain 10dbecause the enzyme could not be puriﬁed in large enou ghquantities. We previously reported the identiﬁcation of the enzyme involved in the initial...

Tài liệu Báo cáo khoa học: An immunomodulator used to protect young in the pouch of the Tammar wallaby, Macropus eugenii pptx

... euge-nin, indicating that eugenin is indeed acting throughCCK2receptors.To further investigate the interaction of eugenin withCCK2receptors, we investigated the effect of eugeninon lymphocyte ... isdue to the release of acetylcholine from cholinergicnerve terminals, the effects of eugenin and CCK-8were determined in the presence of atropine (10)6m).This concentration of atropine was ... concerning the sequence from the C-terminal end of the peptide, while the Y+2 fragmentations (shown schematically under-neath the spectrum) provide sequencing data from the N-terminal end of the...

Tài liệu Báo cáo khoa học: "Using adaptor grammars to identify synergies in the unsupervised acquisition of linguistic structure" docx

... the parses assignedto the training data at the end of sampling, and for the last two epochs we annealed at temperature 0.5(i.e., squared the probability) during sampling in or-4001 10 100 1000U ... sample a parse of thatstring with a PCFG approximation to the adaptorgrammar. This PCFG contains a production for eachadapted subtree in the parses of the other strings in the training corpus. ... segmentation model shown in Figure 1. In this grammar and the grammars below, under-lining indicates an adapted nonterminal. Phonemeis a nonterminal that expands to each of the 50 dis-tinct...

Xem thêm