... stabilization of
the Toc /Tic/ preprotein supercomplex. In this model, Tic1 10 forms the channel protein and also acts in the recruitment of Hsp93 in concert
with the co-chaperone Tic4 0. The TPR domain of Tic4 0 ... 1166–1176 ª 2009 The Authors Journal compilation ª 2009 FEBS
MINIREVIEW
Protein transport in organelles: The composition,
function a...
... monitored by the reduction of
cytochrome c in the presence of NADH at 550 nm [1].
Protein determination
Protein content was determined by the Bradford test [20],
using bovine serum albumin as standard.
PHI ... Lovenberg [23] and Beinert [24], respectively.
Kinetic constants
The catalytic activity of PHR was evaluated both in the
presence and in the absence of...
... cloning
the PCR fragments into the His-tag vector, pET-15b. The
recombinant proteins were generated in the E. coli strain
Origami (DE3), and the N-terminal His-tagged proteins
were purified using ... opening and cell swelling, an event that
is probably reduced in extent by CsA. CsA specifically
inhibits PTP opening by binding to cyclophilin D in
the matrix and on the i...
... located in independent domains
(a b-domain for the N-terminal cluster and an
a-domain for the C-terminal cluster). In the absence of
metals, apo-thionein (apo-T) is predominantly unstruc-
tured and ... composed of a single polypep-
tide chain of 61–68 residues. They are characterized by
a conserved array of 20 cysteines and the absence of
His and aromatic amino a...
... forming eight b-sheets [23]. The motif
was noted on the basis of homology with the C-terminal
region of meprins A and B and the TRAF-C domain,
and, like the BTB domain, facilitates protein protein
interaction ... with
CUL3 and other BTB proteins [2,16], it remains unclear
what kind of interactions are mediated by the MATH
domain in plants.
In this article, we sh...
... answers each. The total cost of producing the
corpus was about $350, consisting of $310 paid in
workers rewards and $40 in Mechanical Turk fees,
including all the trials conducted during the devel-
opment ... documents). The source of Wik-
iXML is the English part of the Wikipedia XML
Corpus by Denoyer and Gallinari (2006). In the
original data some of the...
... suppression of malignant
phenotypes, suggesting the role of miR-9 in the growth
inhibition of malignant cells.
The fundamental function of miRNA is to regulate
their targets by direct cleavage of the ... of tumorigenesis, and may have diagnostic
and therapeutic value in the future.
Materials and methods
Materials
Four pairs of ovarian tissues, including fou...
... organelles.
Therefore it could be used to study the involvement of these proteinases in
protein degradation and the processing of invariant chain. An assay combi-
ning a new monospecific CatE antibody and ... distinguishes between the activities of
the enzymatically similar proteinases, CatE and CatD,
and can therefore be used to investigate the involvement
of the...
... upon the distance between and relative ori-
entation of two fluorophores, being independent of the
strength of protein protein interactions. In the case of
very weak interactions as described in ... citrine) and citrine–GAPDH
(chimeric protein with N-terminal citrine and C-terminal
GAPDH) were localized in the cytoplasm. PGK–ceru-
lean (chimeric protein with N...
... cholesterol, and proteins, is
then stored in the gall bladder. Bile acids excreted into
bile undergo intensive enterohepatic circulation. They
are reabsorbed in the intestine, taken up again by the
liver, ... proteins,
promoting binding of the von Hippel–Lindau tumor
suppressor protein, which acts as the ubiquitin ligase
for HIF-a proteins, leading to the degradation by...