Báo cáo khoa học: Deﬁnition of the residues required for the interaction between glycine-extended gastrin and transferrin in vitro pptx

... Knowledge of the regions of transferrin required for the binding of gastrin, and of the regions in gastrin required for the interaction with transferrin, is obviously essential to a full understanding of ... saturation, suggest the possible involve-ment of the transferrin gastrin interaction in iron homeostasis. In the present work, the interaction between gastrins and transferrin has beencharacterized ... whether iron is required for the Ggly–trans-ferrin interaction, to deﬁne the domains ⁄ residues of Ggly involved in the interaction (using Ggly mutants), and, ﬁnally, to determine the regions of...

Báo cáo khoa học: Structural recognition of an optimized substrate for the ephrin family of receptor tyrosine kinases pot

... sig-naling pathways because of the distinctive mode of interaction between the RTK and the ephrin ligand;cells expressing and presenting the ligand interact withneighboring cells expressing transmembrane ... density for most of the substrate pep-tide, including main chain atoms for the )4 through to the +4 position, but partial or no density for the sidechains of the N-terminal three residues and C-terminaltryptophan ... isoforms (excepting the pseudo-kinases EPHA10 and EPHB6) (Fig. 4), suggesting that these residues are involved more generally in both binding and effec-tive catalysis of the substrate in the...

Báo cáo khoa học: Concerted mutation of Phe residues belonging to the b-dystroglycan ectodomain strongly inhibits the interaction with a-dystroglycan in vitro pot

... positions 691 and 719 [15]. In order to identify the speciﬁc amino acids within the linear interacting epitopes involved in the complex between a-DG and b-DG, alanine scanning of some of the residues ... afﬁnity between recombinant pep-tides spanning the C-terminal domain of a-DG and the b-DG ectodomain by solid-phase binding assays and SPR, demonstrating that despite the intrinsic pit-falls of ... generated. In the C-terminal region of a-DG, between amino acids 550 and 565, Trp551, Phe554 and Asn555were mutated to alanine. In the a-DG-binding epitope comprising residues 691–719 of the b-DG...

Báo cáo khoa học: Identiﬁcation of alternative promoter usage for the matrix Gla protein gene Evidence for differential expression during early development in Xenopus laevis doc

... role in the epithelium–mesenchymal cellinteractions required for normal differentiation and branching of respiratory components of the lung. In addition, MGP mRNA was consistently found in cellsfrom ... (Fig. 6 and results not shown). Although small, this difference in increase in luciferase activity is consistent with the other results obtained, namely the intensity of the RT-PCR bands and the ... regulator and the vertebrate homologue of the Drosophila pro-tein zfh-1 [19], a factor containing both zinc ﬁnger and homeodomain motifs. It is a 124 kDa DNA-bindingprotein which was initially...

Báo cáo khoa học: Kinetic characterization of methionine c-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and triﬂuoromethionine, a promising lead compound against amoebiasis ppt

... decrease in the kcat for Cys. This implies that the hydroxyl group of Tyr108 of EhMGL1 is actively involved in the b-elimination and c-elimination of the C–S bond, but not the b-elimina-tion of the ... 7.0) containing1mm dithiothreitol and 20 lm PLP, and 3–60 lgÆmL)1 of the recombinant enzymes, at 37 °C for 10 min. The ranges of substrate concentrations for the measurement of kineticparameters ... concentration of 2 mm) and the kinetic parameters of recombinant EhMGL1 and EhMGL2 (Tables 1 and 2). Despite the heterogeneity of the EhMGL1 preparation used in the previousstudy [13], the kinetic...

Báo cáo khoa học: Versatile regulation of multisite protein phosphorylation by the order of phosphate processing and protein–protein interactions pptx

... the kinase) due to competi-tion of the two enzymes for the target. Indeed, there isexperimental evidence for kinases and phosphatasescompeting for binding to their targets [39].Assuming the ... a single kinase.We conclude that knowledge concerning phosphory-lation order and the properties of protein–proteininteractions is crucial for the elucidation of the steady-state and kinetic ... approach starts from the description of the elementary steps of enzyme–targetbinding and catalysis and then uses the rapid-equilibrium approximation for protein–protein interac-tions for a systematic...

Báo cáo khoa học: Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans pot

... understand the mode of binding and interference with enzyme catalysis.As the inactivation of any enzyme involved in ametabolic chain will render the whole chain inoperat-ive, the inactivation of ... contact between the ubiquinone from the innermembrane and the active site of DHODH [23,27].Although there are some differences in processing and association in the organization of the fungal and ... of the six proteinsinvolved in pyrimidine de novo synthesis should result in the same profound effect on the pyrimidine nuc-leotide pools in C. albicans. However, in mammaliancell lines, the...

Báo cáo khoa học: Mycobacterium tuberculosis possesses a functional enzyme for the synthesis of vitamin C, L-gulono-1,4-lactone dehydrogenase doc

... Escherichia coli. The recombinantprotein contained an engineered enterokinase cleavagesite in the junction between the GST tag and the Rv1771 sequence. Upon 3 h of induction of the pDEST15_Rv1771 ... biosynthesis of colla-gen and other hydroxyproline ⁄ hydroxylysine-containingproteins ⁄ peptides; synthesis of secondary metabolites,hormones and cytokines [1]; oxidative protein folding and endoplasmic ... protein band, thus pointing to the absence of a covalently bound ﬂavin moiety in the recombinant product. Moreover, as in the case of the plant l-galactono-1,4-lactone dehydrogenase [12],the...

Báo cáo khoa học: Two short protein domains are responsible for the nuclear localization of the mouse spermine oxidase l isoform pdf

... structuraldifference between the two isoforms is the presence of an extra protein domain in mSMO l, encoded by the exon VIa [10].Comparative analysis of the amino acid sequence of the vertebrate members of the ... mammalian SMOs and that they maycooperate in targeting the mSMOl isoform to the nuc-leus. By means of transient expression of the deletionmutant, mSMOlD, in the murine neuroblastoma cellline, N18TG2, ... removal of the NDA amino acid region abolishes proper nucleartargeting of the mSMOl isoform.ResultsStructural analysis and modeling of vertebrateSMO proteinsComparison of the derived amino acid...

Báo cáo khoa học: "Automatic Acquisition of Ranked Qualia Structures from the Web" potx

... improved the results of the Jaccard measure by about 15%.6We determine this number experimentally as the number of web pages containing the words the and and .891matched. On the basis of these, ... evaluationmeasures. Then we describe the creation of the goldstandard. Further, we present the results of the com-parison of the different ranking measures with re-spect to the gold standard. Finally, ... corre-sponding rankings of the qualia elements for eachqualia structure. This is a similar case to evaluat-ing the ranking of documents within information re-trieval systems. In fact, as done in standard...

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