... the target genes of micro-
RNA124a (miR124a), and imaged the miR124a-mediated repression of
chromosome 14 open reading frame24 (c1 4orf2 4, unknown function) during
neurogenesis, using noninvasive ... miR 124 seed sequence of
c1 4orf2 4 (Fig. 4C).
To quantify the in vitro luciferase activity, represent-
ing the miR124a-directed endogenous expression level
of the...
... presence of modifier genes for the EDNRB
gene, and the expression of the modifier genes may be
influenced by the environmental factors, thereby lead-
ing to the phenotypic variability of WS4. One of such
modifiers ... representation of the promoters of
the EDNRB gene. The EDNRB gene is shown as a line and four arrows show the transcriptional initiation sites of...
... [13,23,28,30–33]. The genetic evidence
for the role of AMPK in the hypothalamus is less clear
because the loss of the AMPKa2 subunit in specific
hypothalamic cell types resulted in the opposite pheno-
type ... entry of fatty acid into mitochon-
dria. Thus, the steady-state level of malonyl-CoA in
these tissues is determined by the relative activities of
ACC and malony...
... terminal in
the sinus gland, as a result of late and progressive
isomerization of the Phe3 of the CHH during the
migration of the secretion vesicles along the axonal
tract [39,41].
The immunohistochemical ... whereas
several somas of the two types were dispersed in the
X organ (Fig. 3A), most likely as a result of arte-
factual displacement of the cell bodi...
... 97% inhibition of
BHLmL2 and BHAb, respectively.
The influence of pH on the activities of the BH
enzymes was investigated in the pH range 6–11. The
pH optima were determined to be in the basic range
(pH ... and
BHAb.
The temperature dependence of the activities of the
BHs was tested in the range 5–56 °C; the apparent
optimum temperatures of BHLmL2 were 25–30 °...
... of the CA D. melanogaster
sequences and, on the other hand, the other
D. melanogaster sequences more closely related to the
CAVI vertebrate isoforms (CAa, CAb and D. melano-
gaster-3) or to the ... was
observed in the cytoplasm of the cells. The staining
appears to be specific of the probe sequence because
the staining is clear with the complementary sequence
to RpC...
... approximated by the sum of
the probabilities of observing the set intermolecular
distances defined in the 3D structure, relative to the
likelihood of encountering such distance in the dataset
of all protein–RNA ... onto the RNA bases nearest the center of
the b-sheet structure of the RRM. We then scored
these sequence-variant structures with the distance-
depe...
... interruption of the geometry
of the recognition helix for either Antp or the TTF-1
HD, the anomalous amide exchange pattern and the
NOE connectivity data of the C-terminal portion of
helix III ... inertia.
In the context of RSDM, the detailed analysis of the
three spectral densities J(0), J(x
N
) and J(0.87x
H
)
allowed us to obtain a rather complete description...
... autophosphoryla-
tion of the Y166F mutant was similar to wild-type
2-Cys Prx. Given the absence of another putative site,
we approached the localization of the phosphoryl moi-
ety by examining the mass spectra of ... 2-Cys Prx
Given the essential role of ATP in the peroxidase
activity, we evaluated changes in the structure of
2-Cys Prx brought about by the concer...
... structure of BmKK4,
the first member of subfamily alpha-KTx 17 of scorpion
toxins. Biochemistry 43, 1246 9– 1247 6.
29 Yuan DD, Han YH, Wang CG & Chi CW (2007) From
the identification of gene organization ... S5. The coelution of the synthetic conomarphin
with d-Trp14 (A) or d-Phe13 (B) with the natural
conomarphin.
Fig. S6. Sequential d
aN(i,i +1)
connectivities in t...