... measure-ment of k*on and k*off. At [LCTI] ⁄ [trypsin] ¼ 1.1 and 2.25 the conversion of LCTI into LCTI* (Fig. 3,curves 2 and 3), allowed the simultaneous computation of koff, k*on and k*off. The ... Based on theseconsiderations, the identification of novel BBI inhibi-tors, either as natural compounds or synthetic pep-tides, and the elucidation of their structural and functional properties, ... NMR and MSspectrometry at acidic pH in the absence of trypsin,might be the result of a concomitant series of factors.They include the nature of the amino acid at positionP2¢ (Gln instead of...