Báo cáo khoa học: NO synthase isoforms speciﬁcally modify peroxynitrite reactivity pptx

... dimer; iNOS, inducible nitric oxide synthase; nNOS, neuronal nitric oxide synthase; NO, nitric oxide; NOS, nitric oxide synthase; NOSoxy, oxygenase domain of NOS;PN, peroxynitrite; RNS, reactive nitrogen ... PNadditions, nNOSoxy and bsNOS lose their ability toactivate PN decomposition, as observed for iNOSoxy[49].0255075100125150175200bsNOSnNOSoxyiNOSoxy Peroxynitrite reactivity upon NOS activationRelative ... However, bsNOS does notshow the same spectral modiﬁcations as mammalianNOSs upon PN activation.NOS isoforms diversely modify PN oxidativepropertiesWe investigated the effect of nNOSoxy and bsNOSactivation...

Tài liệu Báo cáo khoa học: Casein kinase 2 speciﬁcally binds to and phosphorylates the carboxy termini of ENaC subunits ppt

... and in vivo inENaC-expressing Xenopus oocytes. The c subunit is weaklyphosphorylated by this protein kinase on another residue(cT599), and the C tail of a is not signiﬁcantly phosphory-lated ... either[35S]methionine or32Pi. The b subunit could be speciﬁcally immunoprecipitated from oocytes by an anti-HA antibody(Fig. 4A). It was endogenously phosphorylated, and theamount of phosphorylation ... SDS/PAGE.(A) Autoradiogram of35S-labelled proteins immunoprecipitated from ENaC injected (b) and noninjected (–) oocytes. (B) HA-tagged b and bS631Aimmunoprecipitated from35S- and32P-labelled oocytes....

Tài liệu Báo cáo khoa học: Molecular and cellular speciﬁcity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx

... Comp Endocrinol 131,134–142.47 Giulianini PG, Smullen R, Bentley MG & Ferrero EA(1998) Cytological and immunocytochemical study ofthe sinus gland in the Norway lobster Nephrops norvegi-cus. ... However, it is worthnoting that the presence of a d-amino acid in CHHsfrom these last species may be overlooked because no speciﬁc investigation procedure, such as amino acidchiral analysis, ... functions.Gen Comp Endocrinol 95, 387–398.19 Serrano L, Charmantier G, Soyez D, Grousset E &Spanings-Pierrot C (2003) Putative involvement of crus-tacean hyperglycemic hormone isoforms in the neuroen-docrine...

Tài liệu Báo cáo khoa học: Changes in purine speciﬁcity in tandem GAF chimeras from cyanobacterial cyaB1 adenylate cyclase and rat phosphodiesterase 2 pptx

... in thecyanobacterial ACs. In this context, it is noteworthythat the rat PDE2 (rPDE2) GAF tandem, which isalmost identical with the mouse sequence, canfunctionally replace the cyanobacterial ... N6amino group. The inter-actions of the b2–b3 element with the purine appear tobe rather distinct in rPDE2 GAF B, cyaB2 GAF Aand cyaB2 GAF B (Fig. 1C–E). This suggests not onlythat amino acid ... III, we incre-mentally elongated the swapped stretch of amino acidstowards the N-terminal, two amino acids at a time, toidentify the amino acids that impeded cAMP stimula-CyaB2-GAFB-cAMPrPDE2A-GAF-B-cGMP...

Tài liệu Báo cáo khoa học: Structural and functional speciﬁcities of PDGF-C and PDGF-D, the novel members of the platelet-derived growth factors family docx

... no P15692), PDGF-B(accession no. 1109245 A), PDGF-C (acces-sion no AAF80597), PDGF-D (accession no AAK38840), VEGF-A (accession no NP003367) and PIGF-1 (accession no. 1FZV). Red, squared areas ... Cystine KnotDatabase (http://hormone.stanford.edu/cystine-knot).This review focuses on the structure and function ofthe two novel members, PDGF-C and -D, of thePDGF subfamily of the cystine knot ... activated platelets secrete PDGF-A, -B and -C,but not PDGF-D [76]. No endogen PDGF-D mRNAcould be detected [87], indicating that normallyPDGF-D does not play a role in the inﬂammatoryphase. A recent...

Báo cáo khoa học: Comparing the substrate speciﬁcities of cytochrome c biogenesis Systems I and II docx

... Sys-tem II. ResBC is able to attach heme to monoheme c-type cytochromesc550from Paracoccus denitrificans and c552from Hydrogenobacter thermo-philus, both normally matured by System I. The production ... andCcdA [12,13] (Fig. 1). Notably, the genomes of someBordetella species, e.g. B. parapertussis, encode bothSystems I and II [1].In common with System I, it is not clear whetherheme is transported ... an as yetunidentified, novel biogenesis system. All fullysequenced genomes of organisms expressing such sin-gle-cysteine cytochromes lack identifiable homologuesof known c-type cytochrome biogenesis...

Báo cáo khoa học: Phytoene synthase genes in tomato (Solanum lycopersicum L.) – new data on the structures, the deduced amino acid sequences and the expression patterns doc

... Psy1 and Psy2 relative tran-script contents were normalized onto the 18S rRNA(endogenous reference) transcript contents and com-pared to normalised petal transcript content (calibra-tor), ... contents were normalized onto the endogenous refer-ence transcript (18S rRNA) to account for differences inthe amount of total RNA content among samples and com-pared with the normalised transcript ... (2004) Solan-aceae – a model for linking genomics with biodiversity.Comp Funct Genomics 5, 285–291.3 Peralta IE, Knapp S & Spooner DM (2006) Nomencla-ture for wild and cultivated tomatoes....

Báo cáo khoa học: pH dependence, substrate speciﬁcity and inhibition of human kynurenine aminotransferase I ppt

... amino acids or other keto acids onhKAT-I catalyzed KYNA formation. Analysis of substratespeciﬁcity revealed t hat a number of amino acids a nd a-ketoacids can serve as the amino group donor ... Guidetti, P., Okuno, E. & Schwarcz, R. (1993)Characterization of human b rain k ynurenine aminotransferasesusing [3H]kynurenine as a substrate. Neuro scienc e 55, 177–184.26. Okuno, E.F ., ... rine pyruvate aminotransferase. FEB S Lett.527, 1 99–204.29. Baran, H., O kuno, E ., Kido, R. & Sc hwarcz, R . ( 1994) Puriﬁca-tion and c haracterization of kyn urenine aminotransferase I...

Tài liệu Báo cáo khoa học: LAT – an important raft-associated transmembrane adaptor protein pptx

... S, Notarangelo LD & Samelson LE(1999) Linker for activation of T cells (LAT), anovel immunohistochemical marker for T cells, NKcells, mast cells, and megakaryocytes: evaluation innormal ... association of LAT with phosphatidylinositol3-kinase and phospholipase C-c following CD2 stimu-lation. Eur J Immunol 32, 2188–2198.145 Umehara H, Inoue H, Huang J, Kono T, Minami Y,Tanaka Y, Okazaki ... 262amino acids (human) or 242 amino acids (mouse). Ashorter human isoform exists (233 amino acids), whicharises by alternative splicing and lacks residues 114–142 of the long form. So far nothing...

Tài liệu Báo cáo khoa học: Helicases ) feasible antimalarial drug target for Plasmodium falciparum pptx

... falciparum genome in 2002, new opportunities forresearch have arisen [15]. The P. falciparum genomehas 14 chromosomes, a linear mitochondrial genomeand a circular plastid-like genome [15,16]. ... compoundstested, including daunorubicin and nogalamycin, islowest for helicases from P. falciparum compared withthe other helicases [29,30]. It is interesting to note thatinhibition by nogalamycin is highly ... parasite-speciﬁcsequences could still be speciﬁcally targeted becausethe antibodies to PfDH60 do not cross-react with thehuman p68 [17,19–21].To the best of our knowledge only a few helicaseshave...

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