... 15 mA per gel for 1 h 30 min and50 mA per gel for 5–6 h at 15 °C. For preparative gels,1 mg of protein was solubilized in 2 mL of buffer for 1 hat room temperature, centrifuged for 30 min at ... protein spots.Unfortunately, error-prone matching to wrong refer-ence spots is often underestimated, making quantita-tive statistical information useless. Hence, annotationsof interesting candidate ... pre-fractionation techniques (i.e. select-ing for cysteine-containing peptides or glycoproteins)may allow for higher resolution capacity but at thecost of information loss. For example, using ICATlabelling,...