... critical role of the lastamino acids in the C-terminus of MaL. Deletion of the four last amino acids strongly reduced the expression of the protein in T. reesei and S. cerevisiae. Further-more, the ... The C-terminus of MaLblocks this route, as the packing of the C-terminusagainst the tunnel is extensive, and there is no space for dioxygen or any other molecules to enter. Further-more, the ... folding of the protein during the post-translational processing,and thereby the function of the mature laccase.Our structural analysis of wild-type MaL indicatedthat the last four amino acids of the...