Báo cáo khoa học: Amyloid oligomers: formation and toxicity of Ab oligomers ppt

... characteristics of Ab oligomers and their formation mechanismremain unclear.Structure and size of soluble Ab oligomers Many types of natural and synthetic Ab oligomers of different sizes and shapes ... elucidatethe mechanism of formation of Ab oligomers. Possible mechanism of solubleoligomer formation and toxicity The mechanism of formation of soluble Ab oligomerin vivo remains unclear. Glabe et al. ... FEBSMINIREVIEW Amyloid oligomers: formation and toxicity of Ab oligomers Masafumi Sakono1,2 and Tamotsu Zako11 Bioengineering Laboratory, RIKEN Institute, Wako, Saitama, Japan2 PRESTO, Japan Science and...

Tài liệu Báo cáo khoa học: Substrate speciﬁcity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt

... 85% inhibition of BHLmL2 and BHAb, respectively, while 1 mm 2-mer-captoethanol caused 85% and 97% inhibition of BHLmL2 and BHAb, respectively.The influence of pH on the activities of the BHenzymes ... molecular mass of about 120 kDa. Thus, these data suggest that nativeBHAb is a dimer.Identification and chemical modification of thepurified enzymesTo identify BHLmL2 and BHAb, the bands obtainedfrom ... 55% and 51%loss of the initial activities of BHLmL2 and BHAb,respectively. These results strongly suggest that Ser isinvolved in the catalytic activity of BHLmL2 and BHAb and are consistent...

Tài liệu Báo cáo khoa học: Identiﬁcation, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx

... be able to transfer protons [31].However, without an overexpression approach of RpCAbr and RpCAtr, we cannot know the functionaleffect of changes of some key amino-acids.Origin and number of ... ﬂuorochrome labeling of the probes was chosento detect RpCAbr mRNAs and RpCAtr mRNAs by green and red ﬂuorescence, respectively. The antisense RpCAbrprobe was synthesized by incorporation of DIG-conjugatedUTP ... previously sequenced RpCAtr (BPNJ¼ 100 and BPMP¼ 99). Fungia scutaria (FCA-a and FCA-b) and Caenorhabditis elegans (CA1 and CA2) sequences falloutside of clade I and are more closely related to eachother...

Tài liệu Báo cáo khoa học: ATP-dependent modulation and autophosphorylation of rapeseed 2-Cys peroxiredoxin docx

... (A)Expanded view of peaks at m ⁄ z 2800.36 and 2832.35 and the fragmentation of the peak at m ⁄ z 2800.36. (B) Expanded view of peaks atm ⁄ z 2934.36 and 2950.35 and the fragmentation of the ... C53S 2-Cys Prx. Expanded view of peaks at m ⁄ z 2800.36 and 2832.35 and thefragmentation of the peak at m ⁄ z 2832.35. 2-Cys Prx was digested with trypsin and prepared for MALDI-TOF MS as described ... incorporation of the phos-phoryl moiety. Consistent with this, Fig. 3C showsthat a serine in place of Cys53 and Cys175 retained and abrogated, respectively, the ability to incorporatethe32P-label...

Tài liệu Báo cáo khoa học: Preliminary molecular characterization and crystallization of mitochondrial respiratory complex II from porcine heart ppt

... preparation of complex II. The four subunits of complex II are labeled on the left, and the positions of molecular weight standards are indicated onlane 2.X. Huo et al. Characterization of respiratory ... requirements of struc-ture determination, we had to measure the gene sequences and full amino acid sequences of the porcine complex IIsubunits. An abundance of gene sequences of mammaliancomplex ... G250, and the four bands corresponding tosubunits of porcine complex II were cut out and N-terminalsequenced by the Edman degradation method and amino acidanalyzer in the Department of Biology,...

Tài liệu Báo cáo khoa học: Unusual metal specificity and structure of the group I ribozyme fromChlamydomonas reinhardtii23S rRNA pptx

... 23S.5DGbpre-RNA, and the binding of a minimum of n Mg2+ions leads to formation of theactive complex, whereas binding of a minimum of mMn2+ions forms the inactive complex. The sizes of the active and ... the absoluteconcentrations; a ratio of Mn2+⁄ Mg2+ of approxi-mately 1 : 2 or higher inhibited G-dependent cleavage of 23S.5DGbRNA (and self-splicing of 23S.5, notshown).Quantiﬁcation of ... majority of product comes from the highly active fraction. Hence, plots of the percentage of total RNA that is InDGbyield n and m from Eqns 5 and 6.T C. Kuo et al. Mn2+inhibition and structure of...

Tài liệu Báo cáo khoa học: Physico-chemical characterization and synthesis of neuronally active a-conotoxins docx

... spectrometry and the lability of the sulfogroup in mass spectrometryanalysis allows recognition of the modification, and differentiation of sulfotyrosine and phosphotyrosine [38].Characterization of the ... verification of tyrosine sulfation ina-PnIA and a-PnIB and revision of those sequences weremade in an investigation of labile sulfo- and phospho-peptides by electrospray MALDI and atmosphericpressure ... the diversity and utility of the pool of available a-conotoxins. Their identificationandcharacterizationdependonasuiteoftechniqueswith increasing emphasis on mass spectrometry and micro-scale...

Tài liệu Báo cáo khoa học: "Beefmoves: Dissemination, Diversity, and Dynamics of English Borrowings in a German" ppt

... in one of the German and English CELEXwordlists are automatically classified as such. Thisimproved classifier results over tokens and types, asseen in Table 1 in the comparison of token and typeprecision ... comparison of DU, DT,log10f with ∆ log10fquency (log10f). DU=Uw˜Uwis defined as the ratio of the actual number of users of word w (Uw) overthe expected number of users of w (˜Uw), ... findings that DU and DTserve better than fre-quency as predictors of word fate, for the set of an-glicisms (Table 3), all correlations were both nega-tive and stronger, and initial frequency...

Tài liệu Báo cáo khoa học: "Multi-Component TAG and Notions of Formal Power" docx

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Tài liệu Báo cáo khoa học: "NLP for Indexing and Retrieval of Captioned Photographs" pot

... (Veltkamp and Tanase, 2000). Content-based indexing and re-trieval of images is based on features such ascolour, texture, and shape. Yet, image understand-ing is not well advanced and is very ... "knife on table" vs. "blade on bar counter", and "cable around neck" vs. "neck with cable re-moved" respectively.SOCIS consists of a pipeline of processingresources ... Ontology and Indexing TermsWe have made use of the British Police Infor-mation Technology Organisation Common DataModel and a collection of formal reports producedby scene of crime officers...

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