Báo cáo khoa học: Crystal structure of salt-tolerant glutaminase from Micrococcus luteus K-3 in the presence and absence of its product L-glutamate and its activator Tris pdf
Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf
... at its N-terminusinstead of a free amino group [32]. Therefore, the asparagine in position 26 was labeled within the intactprotein at its a-amino group and represents the aminoterminal amino ... the amino terminus of the pro-tein. Using this method, we showed that asparagine 26represents the amino terminus of P40. Knowing, that the first amino acids of the processed P40 and P90 is the ... (CAF) [30]: during the first step of the labelingreaction the e-amino groups of lysine are specificallyconverted to homoarginine. In a second step, the freeamino terminal groups of the peptides...
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Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt
... its native structure after the inser-tion of foreign sequences and in the presence and absence of Ca2+ions, so that the in uence from the host protein to the inserted sites is minimized [14]. ... was introduced to perturb the Ca2+-binding ability of the grafted EF-hand of STIM1.(B) Modelled structure of the engineered protein with the grafted EF-hand Ca2+-binding motif (magenta) from ... grafting approach to address thisquestion. As shown in Fig. 1B, the distance between the two termini of the inserted Ca2+-binding sites in the model structure of the EF-hand of STIM1 is within15...
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Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt
... form the phenolic substrate-binding site of the enzyme, whereas the T2 and the T3-pair coppers form the trinuclear site that is responsible for binding and reduction of the molecular oxygen. The ... which is Asp in TaLcc1, and Glu in MaL. In addi-tion, the crystal structure of TaLcc1 does not have a chloride attached to the T2 copper, as observed in the crystal structure of MaL. The unique ... against hydrolysis, and improving the Fig. 1. (A) The crystal structure of TaLcc1as a surface model. Domain A is presented in blue, domain B in green, and domain C in yellow. The N-glycans are...
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Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt
... determined the X-ray crystal structure of a truncated form of importin-a lacking the importin-b binding domain, bound to a CLIC4NLS peptide. The NLS peptide binds to the major binding site in anextended ... cargo binding sites, referred to as the major and minor binding sites [1]. These sites arelocated in the concave face of the protein near regions of invariant Trp and Asn arrays. The major bindingsite ... significant interactions with importin-a.This primarily occurs by the formation of a hydrogenbond between the side-chain hydroxyl group of Tyr205 and the importin-a main chain at the C-terminus of the...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... an independent metal-binding siteat the interface of the two domains, which consists of four side chains: two (His31 and His79) from the N-terminal domain and two (Asp165 and His169) from the ... residuesinvolved in the binding of the metal cofactor.ApeSODs in different forms were superimposed with the least square fit of Ca atoms of the residues in the range10–200. ApeSOD and P. shermanii ... for the first time, we describe the crystal structure of ApeSOD. In particular, we focuson the coordination of the metal cofactor in the activesite as well as the changes it experiences in response...
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Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc
... is the only direct contactbetween the protein backbone and the substrate. The side chain of Thr292 is found to recognize the 2-phos-phate in both the binding model of PhyK and the crys-tal structure ... in the acidic range. They consist of two domains, a large a ⁄ bdomain and a small a domain with the catalytic site at the interface of the two domains [4,5]. HAPs can initi-ate hydrolysis of ... AppA involvesGlu219. The side chain of this residue is pushed out of the catalytic pocket upon phytate binding. Here,PhyK mimics the phytate-bound structure of AppA,even in the absence of sulfate...
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Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx
... on the remaining subgroups of the HAT ⁄ HAT-like ⁄ DESC and corin subfamilies exists. We therefore cloned,expressed and purified the serine proteinase domain of DESC1 and solved the crystal structure ... cystein-rich(SRCR) domain, which was rigidly bound to the back of the proteinase domain in the crevice between the C-terminal helix, the 204-loop and the 126-loop [7]. The C-terminus of hepsin ... on the opposite side of the proteinase domain from the active site cleft. It seems very unlikely, there-fore, that the SEA domain would directly in uence the binding of either substrates or inhibitors...
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Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc
... role in the enzymaticreaction which will be discussed later. The structure of the active site, the type of proteinligands, the zinc-binding motif, the presence of a waterFig. 3. Structure of the ... residue in the zinc-bound MshB structure (PDB code 1Q74) adopts a sim-ilar strained conformation. On the other hand, in the absence of a zinc ion in the active site, such as in the structures of the ... that the metalmust be intrinsically contained in the protein. The structure of the active site is illustrated in Fig. 3. The zinc ion is tetrahedrally coordinated bythree protein residues and...
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Tài liệu Báo cáo khoa học: Crystal structure of a subtilisin-like serine proteinase from a psychrotrophic Vibrio species reveals structural aspects of cold adaptation docx
... surfa-ces. Furthermore, the substrate-binding cleft of 1SH7differs from that of 1IC6 and 1THM in shape, beingseemingly deeper and more distinct, and in being morenegatively charged than the binding ... ongoing mutagenic research in which single and combined amino acid substitutionsaimed at increasing the stability of the Vibrio protein-ase are being tested. Elucidation of the Vibrio protein-J. ... vulgaris.Results The crystal structure of the Vibrio proteinase The obtained Vibrio proteinase crystals formed clusters of needles, which transformed into thin platelets withina few days. The crystals...
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Tài liệu Báo cáo khoa học: Crystal structure of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase complexed with an analogue of 1,3-bisphospho-D-glyceric acid Selective inhibition by structure-based design docx
... sequences and superimpos-able 3D structures [24]. Parallel studies of these effectsallowed identification of the specific interactions between the inhibitors and the proteins. In the absence of a 3D structure ... the phosphorylation step, Arg249 may induce compression of the product, to set it on its way for expulsion from the activesite (or its introduction into the active site of the substrate in the ... improved activity of this compound may be a result of hydrogen bondingbetween the hydroxyl of Thr167 of the protein and the amino group of compound 8. No other inhibitor offeredsuch an interaction....
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