Báo cáo khoa học: Neural retina leucine-zipper regulates the expression of Ppp2r5c, the regulatory subunit of protein phosphatase 2A, in photoreceptor development pdf

... ª 2010 FEBS Neural retina leucine-zipper regulates the expression of Ppp2r5c, the regulatory subunit of protein phosphatase 2A, in photoreceptor development Jung-Woong Kim, Sang-Min Jang, Chul-Hong ... S-transferase;NRE, neural retina leucine-zipper- response element; Nrl, neural retina leucine-zipper; NS, not signiﬁcant; P, postnatal day; PP2A, protein phosphatase 2A; siRNA, small interfering RNA; WT, ... blotting.Fig. S1. Expression of neural retina Nrl mRNA.Fig. S2. Generation of the polyclonal antibody for Nrland expression of Nrl proteins in various cell lines.Fig. S3. Efﬁciency of Nrl...

Báo cáo khoa học: [Na+]i-induced c-Fos expression is not mediated by activation of the 5¢-promoter containing known transcriptional elements ppt

... elevation of the [Na+]i⁄ [K+]iratio [7].Keeping in mind the increase of total RNA synthesisseen in ouabain-treated VSMCs [6], we proposed that the expression of antiapoptotic genes, including ... localize the putative Na+response element (NaRE) within the 5¢-UTR containing all known elements involved in the regulation of c-Fos expression. Results The effect of ouabain on c-Fos expression is ... that5 h of inhibition of Na+⁄ K+-ATPase with ouabainled to an  10-fold elevation of intracellular exchange-able Na+ in all types of cells investigated. In contrast, the action of ouabain on...

Tài liệu Báo cáo khoa học: Disulﬁde bridge regulates ligand-binding site selectivity in liver bile acid-binding proteins ppt

... T2values in the apo protein and in all of the investigated holo proteins,thus plays a key role in regulating the positioning of the helix–loop–helix motif with respect to the b-barrel in order ... whereas the bound GCA mostly in uenced the C-terminal region of the protein, in agreement with the site selectivity observed for the twoligands. Interestingly, the heterotypic complex, in which the ... cou-pled in different ways, whether in the presence or in the absence of the ligands [10]. Another source of ligand-binding variability may be introduced by the presence of disulﬁde bridges. Indeed,...

Báo cáo khoa học: Amino acid limitation regulates the expression of genes involved in several speciﬁc biological processes through GCN2-dependent and GCN2-independent pathways ppt

... that the mecha-nism underlying the recognition of protein quality actsthrough the sensing of free circulating amino acidsresulting from the intestinal digestion of proteins [4,5].Another ... contributeto these multistep processes, including the metabolicconditions of the cell, nature of the stimulus, RNAbinding factors and the sequence of the target mRNA[35].Another amino acid sensing ... original French government works in the regulation of gene expression in response toamino acid starvation. However, comparison of the regulatory mechanisms involved in the control of geneexpression...

Báo cáo khoa học: a-Fetoprotein positively regulates cytochrome c-mediated caspase activation and apoptosome complex formation docx

... processing in the presence of a low suboptimal dose of cyt-c and requires the presence of all members of the apoptosome complex toinitiate this process. We examine the mechanisms by whichAFP regulates ... independent of membrane receptorsignaling [20–23]. We investigate here the intracellularmolecular pathways of the AFP-mediated triggering of apoptosis. To analyse the involvement of cyt-c release in AFP-mediated ... by the reduced expression of Apaf-1 protein in resistant leukemic cells [48]. A highbackground level of cytosolic cyt-c has been shown in vivo in the aging heart, with a signiﬁcant decrease in...

Báo cáo khoa học: "Neural Network Probability Estimation for Broad Coverage Parsing" doc

... bias in training recursively defined neural networks. The only trained parameters of the mapping h are the parameters of the functiong, which records a subset of the information froma set of ... applied at the be-ginning of training but reduced to zero by the end of training.7A11 our results are computed with the evalb program fol-lowing the now-standard criteria in (Collins, 1999).Length<40AllLRLP ... where the information is input and the place where the infor-mation is needed, the less likely the training is tolearn to record this information. We can exploitthis recency bias in inducing...

Báo cáo khoa học: TORC-SIK cascade regulates CREB activity through the basic leucine zipper domain pdf

... [9]. In addition to the DNA binding, regions involved in the nuclear import and export of CREM have beenmapped in the bZIP domain [10]. The bZIP domain of CREB interactswith a variety of cellular ... steroidogenicacute regulatory protein (StAR) and side chain clea-vage P450 (CYP11A1), rose as SIK1 expression declined. Overexpression of SIK1 in Y1 cells lowered the level of the cAMP-induced expression of the ... within their bZIPs andcore sequences in the transactivation domain, knownas the kinase inducible domain (KID). Serine residue133 (Ser133) in the KID of CREB and the equival-ent residues of...

Báo cáo khoa học: "Neural Network Recognition of Spelling Errors" docx

... drawbacks, though: they require much more training time and use part of their processing capability for the development of representations, rather than the problem to which the network is applied, ... available space in the FLLB. Instead of leaving certain letter positions blank, as in a split representation, extra letter positions are filled by continuing to add letters from the beginning and ... containing many-to-many mappings. The testing phase of ARTMAP-ICMM outputs a rank-ordered list of potential mappings, with the rank of the desired output returned as a score. A score of 1...

Tài liệu Báo cáo khoa học: High-afﬁnity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx

... Seurin D, Segovia-Quinson B, Hardouin S& Binoux M (1986) Analysis of serum insulin-like growthfactor binding proteins using western blotting: use of the method for titration of the binding ... binding must only be a localeffect because binding of PMP-BSA, which binds toother sites in the ectodomain of these heterodimers,resulted in the predicted amount of ligand binding.This loss of ... the I1572T mutation residing in the heart of the IGF-II binding domain in repeat 11 disruptsIGF-II binding to the receptor [22,31–33]. The ligandblotting data shown in Fig. 1C conﬁrm that the...

Tài liệu Báo cáo khoa học: 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5¢-phosphate synthases of fungi and archaea docx

... were of two types, either with the two-domain architecture of the RibG protein of Bacil-lus subtilis or the RibD protein of E. coli, respectively[18–20], or the single-domain type of 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone ... reductase domain islocated at the N-terminus and the predicted deaminaseat the C-terminus of the bifunctional protein. In plants, orthologues of pyrimidine reductases of the riboﬂavin pathway ... Enzymaticsynthesis o f guanine n ucleotides labeled w ith15Natthe2-amino g roup of the purine ring. Anal Bi ochem 225, 18–23.27 Shaw E (1950) A new synthesis of the purines adenine,hypoxanthine,...

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