... envelope
One of the goals of the present study was to deter-
mine the cleavage sites of hatching enzyme on egg
envelope proteins. The natural substrate of hatching
enzyme is fertilized egg envelope ... discuss the
mechanism of egg envelope digestion by hatching
enzyme, mainly based on the structural characteristics
of egg envelope prote...
... accompa-
nied by a reduction in the amount of cytosolic Sp1.
In order to determine whether glutamine increases the
binding of Sp1 to the SREBP-1a promoter region
containing the Sp1-binding elements, we performed ... containing a
mutated sequence of the SRE, pSQS containing the human
SQS promoter, pSQS SREKO containing two mutated
SREs, pLDLR containing the human LDL re...
... with other macromolecules as part of the
PCSK9-mediated degradation of the LDLR. A cluster
of residues on the conserved protrusion is involved in
the binding of PCSK9 to the EGF-A domain of the
LDLR, ... detected in any urochordate, e.g. in the
fairly well-studied Ciona intestinalis, or in any other
invertebrates. The C-terminal domain of PCSK9, the
CRD,...
... To
distinguish total binding from non-specific binding, cells were
incubated for 10 min in either PBSCM alone or PBSCM
containing 200 lm cocaine for inhibition of DAT or 200 lm
S-citalopram for inhibition ... Values are the
mean of six replicates. The specific uptake was determined by
subtracting the uptake counts in the absence of inhibitor
from the uptake counts in the...
... 2A). The zinc ions are required
for the structural integrity of the protein, as mutation of
any of the cysteine residues involved in zinc coordina-
tion results in protein unfolding [22]. The ... include
the MLL1 CXXC domain [22], a portion of the MLL1
TAD bound to the KIX domain of the cAMP
response element-binding (CREB) binding protein
(CBP) [23], a peptide...
... examining the role
of GIF in AD, linked to its initial discovery as a factor
deficient in the AD brain. There remains no clear con-
sensus on the role of GIF in the pathogenesis of AD,
and therefore ... in
1991, sparked considerable interest in understanding
the role of all MTs in the brain, and particularly
within the injured or diseased brain. In the cas...
... repre-
sent the last subunits incorporated into the bc
1
com-
plex. On the other hand, the absence of Qcr9p in the
DQCR9 ⁄ DQCR10 strain prevented the binding of ISP
(Fig. 4B). Interestingly, in the ... comparable amounts in
both yeast strains. Therefore, the reason for the disap-
pearance of the bc
1
dimer in the yeast strain in which
Qcr10p is missing r...
... and GH13 enzymes is the
lack of domain B protruding out of the TIM barrel in
the place of loop 3 connecting the b3-strand to the
a3-helix [9,21]. Sharing domain B by rBATs and
GH13 enzymes, and ... domain,
including domain B and the C-terminal domain C for
rBAT proteins (669 residues on average); (b) the cata-
lytic TIM barrel domain, including domain B and the
C-termi...
... from
C. intestinalis expressed in vitro and native
enzyme expressed in vivo indicates the enzyme
is AChE
To determine the nature of the cholinesterase activity
of the recombinant C. intestinalis enzyme ... with the PRAD domain
of ColQ indicates that the WAT domain of the
C. intestinalis AChE is capable of organizing the sub-
units into a tetramer through in...
... responsible for
the calcium binding [8], we hypothesize that this bind-
ing event takes place at the C2 domain.
A second calcium-binding site of lower affinity at
the C2 domain (corresponding to the dissociation
constant ... belongs
to the C2 domain (141 amino acids) with its extended
loops, whereas the lid-like region at the top represents
another flexible part of the struc...