... present during the incubation (data not shown).As Cat only partially prevented enzyme inactivation,EhPFOR inactivation by H2O2was examined in detail. The enzyme was strongly inhibited in a dose-dependentmanner ... DaPFOR,which is naturally resistant to inactivation under aero-bic conditions, contains an extra domain at the C-ter-minal region that spans the vicinal subunit of the dimer and that overlays the Fe–S ... indicatedthat, under saturating O2conditions, the enzyme wasfully inactivated after a short incubation (30 min). At this time, EhPFOR inactivation could be reversed to a great extent by incubation...