... Research Resources, a research award from
JDRF, a grant from ADA, and a research grant from
OCAST HR07-067.
References
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36 Bok D, Ruiz A, Yaron O, Jahng WJ, Ray A, Xue L &
Rando RR (2003) Purification and characterization of a
transmembrane domain-deleted form of lecithin retinol
acylt...
... radiation facilities and we would like to thank Dr
P. Carpentier for assistance in using beamline BM3 0a.
References
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coccus thiocyanatus a ... sulfate.
X-ray diffraction data of the wt and M100K crystals
were collected on an in-house beam using a MAR345
Image Plate detector. The crystals were mounted in a capil-
lary and datasets...
... RCL, with
attached protease, as an additional strand into b-sheet A of
the inhibitor, causes a dramatic conformational change in
the serpin [9]. Recent X-ray crystallographic diffraction
analysis ... vivo
clearance of the T–AT complex in rats
Thrombin, native AT, and elastase-cleaved AT were labeled
with
125
I (Amersham Pharmacia AB, Uppsala, Sweden)
using the chloramine T method, acc...
... wild-type a1 AT in a manner that was
comparable with inhibition of cationic and anionic
trypsins, demonstrating that Arg198 is the critical
determinant of resistance against a1 AT (Fig. 2A, B).
Figure 2A ... Mesotrypsin associates with a1 AT Pittsburgh with an SI
of 2, and the calculated k
a
is 1.1 · 10
6
M
)1
Æs
)1
. Using purified pan-
creatic cationic and anionic trypsins and...
... 5¢-GGTATTGAGGGTCGCCATGGTTATGTTC
AATCACCA-3¢; reverse primer, 5¢-AGAGGAGAGTTAG
AGCCTTACAAGAAGGGTCCAAAGA-3¢). The PCR
product was purified, treated with T4 exonuclease to
create vector-compatible overhangs and annealed to a
prepared ... chitinolytic
machineries, such as in the lactic acid bacterium
(LAB) Lactococcus lactis ssp lactis IL1403. LABs are
Gram-positive, facultatively, anaerobic,...
... Healthcare).
Caspase 9 activity assay
Caspase 9 activity was examined according to the instruc-
tion manual of the Caspase 9 ⁄ Mch6 Fluorometric Protease
Assay kit (Medical and Biological Laboratories ... The Bcl-2 protein family:
arbiters of cell survival. Science 281, 1322–1326.
38 Tsukahara T, Kannagi M, Ohashi T, Kato H, Arai M,
Nunez G, Iwanaga Y, Yamamoto N, Ohtani K,
Nakamura M et...
... antibodies
were as follows: anti-porin from Calbiochem, anti-HA from
Covance BabCo, anti-mouse and anti-rabbit secondary
antibodies from Bio-Rad Laboratories and Amersham
Pharmacia Biotech, respectively. Antibodies ... S-HA. The
blots were probed with antisera against HA, with anti-porin, and with
two other antisera against complex I proteins (MWFE and PSST).
(B) BN/PAGE. Top panel: his...
... short-lived transient protein
protein interactions by intermolecular nuclear paramag-
netic relaxation: plastocyanin from Anabaena variabilis.
J Am Chem Soc 125, 6858–6859.
Thermodynamic parameters ... thermodynamic parameters for the
haems calculated from the NMR data are relative, it
could be argued that all haem potentials had been
modified to a similar degree but to an unknown extent...
... Enhanced Ras maturation by heat stress
was associated with a heightened activation of extra-
cellular signal-regulated kinase (ERK), a key mediator
of both mitogenic and stress signaling pathways, ... heat-induced
plasma membrane fluidization, are indeed capable of
activating HSP formation even at the growth tempera-
ture, without causing measurable protein denaturation.
We also de...
... 2004
hydrolases (Fig. 3). These enzymes share a functional
catalytic triad made of a catalytic nucleophile serine,
associated to a proton c arrier histidine and a c harge r elaying
aspartic (or glutamic) ... and characterization of the protein Rv1399c
from
Mycobacterium tuberculosis
A novel carboxyl esterase structurally related to the HSL family
Ste
´
phane Canaan
1
, Damien Ma...