... hexamer. PCR was per-formed with PCR master mix (Roche, Indianapolis, IN,USA) at 94 °C for 5 min followed by 35 cycles of 94 °C for 30 s, 48 °C for 30 s and 72 °C for 30 s using a set of degener-A ... broken line arrows), andRT-PCR was performed with Taq DNA polymerase(Roche) at 94 °C for 5 min followed by 35 cycles of 94 °C for 30 s, 58 °C for 30 s and 72 °C for 30 s. The sizes of thePCR products ... RPE65c-Rev; con-taining a HindIII site) (Table 1) at 94 °C for 5 min fol-lowed by 35 cycles of 94 °C for 1 min, 58 °C for 1 min and72 °C for 2 min. After agarose gel electrophoresis, the PCRproduct...