... fusionproducts. Individual synthetic Calb EF-hands I, III, IVand Vbind calcium [29]. This data of A˚kerfeldt et al. [29] agreeswith the large body of independent data collected on Calb and truncated Calbs ... (h) and other amino acids (.), thecalcium-binding ligands are designated by their coordinates: x, y, z, -y, -x and -z [60]. Residues discussed in the text are given in bold (G34,K60 and G81 of ... preparedin 50 mM deuterated Tris, 25 mM NaCl pH 8.1. Thestimulated-echo with longitudinal eddy current delay(STE-LED) method was applied to obtain diffusion orderedspectroscopy (DOSY) spectra...