... amino-acid composition contribute to the
thermostability of
Bacillus licheniformis
xylose isomerase
Claire Vieille
1
, Kevin L. Epting
2
, Robert M. Kelly
2
and J. Gregory Zeikus
1
1
Department of Biochemistry ... example,
thermostable xylose isomerases (XIs) (EC 5.3.1.5), which
catalyze the isomerization of
D -xylose to D-xylulose in vivo,
are used for the...
... The phosphoryl and pyridoxyl moieties
of the P-pyridoxyl group are shown in red and
magenta, respectively. The fractional solvent
exposed area of the P-pyridoxyl group is 0.39
and 0.082 for the ... P-pyridoxyl group to the solvent.
The fluorescence decay of pyridoxamine and of the
P-pyridoxyl-labeled protein were monitored at 393 nm
upon excitation at 326 nm....
... A
˚
ke
Engstro
¨
m at the Peptide Synthesis and Analysis Laboratory
of the Department of Medical Biochemistry and Microbio-
logy, Uppsala University. The SP6 primer 5¢-ATT TAG
GTG ACA CTA TAG-3¢ and the three ... was
thus similar to that of the porcine liver enzyme.
The specificity of the recombinant enzyme towards
phosphohistidine was supported by the finding th...
... production of cystatin F compared
to the ubiquitous inhibitor, cystatin C, we measured the
cystatin contents in cell lysates and culture media of cells
grown for 2 and 4 days with cystatin F and cystatin ... of the cystatin F gene and promoter. The structures of the human cystatin F (CST7)andcystatinC(CST3) genes are illustrated
schematically at the top. In the...
... a nonglycosylated triplet at position Asn70 [48].
The majority of the peroxidases carry one or two putative
glycans. Seven appear to be nonglycosylated. Therefore, the
high number of glycans found ... [64] have studied the promoter
activity and wound-induction of HRPC and -E types, and
of AtPCa and AtPEa. Most remarkably, C2, and only C2,
responded strongly to wo...
... as the standard.
RESULTS
Induction of citral lyase
Initially, the reproducibility of the results was hindered by
variations in the citral lyase activity of the P. digitatum
spores. Remarkably, ... hydratase and aldolase activity
in a single enzyme. No other enzyme has been reported
to catalyse the conversion of citral into methylheptenone
and acetaldehyde, or a simil...
... endothelial
layer of the artery was removed by bubbling with oxygen
for 10 s. Absence of the endothelial layer was confirmed by
the lack of relaxation in response to a 30-min perfusion of
acetylcholine ... This sensitivity is well within the capability
of the ES-MS system employed.
The primary structural diversity displayed within
amphibian skin bradykinins may not simp...
... localized in the cytoplasm (Fig. 6, middle panel). The
GFP-tagged TRAF domains of TRAF2 and TRAF3
also localized to the cytoplasm whereas the TRAF
domain of TRAF1 showed nuclear and cytoplasmic
localization ... implicated
mainly in signaling by members of the TNF receptor family,
TRAF6 primarily transduces signals initiated by IL1/Toll
receptors. In particular, TRAF4 has...
... host
naturally devoid of phosphatidylinositol (PtdIns). In the
context of the bacterial membrane and in addition to de novo
synthesis, the plant enzyme is capable of catalysing the
exchange of the inositol ... Phosphatidylinositol synthesis and exchange of the inositol head
are catalysed by the single phosphatidylinositol synthase 1
from
Arabidopsis
Anne-Marie Ju...
... previously
shown that at the beginning of the stationary phase the rate
of synthesis of heat-shock proteins increases considerably,
but only transiently [2,18]. Similarly, we observed that the
steady-state ... the deletion of the UP12 encoding gene increased
the sensitivity of the mutant to CCCP (Fig. 6), it did not have
any detectable effects on the sensitivity...