Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc

Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc

Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc

... amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase Claire Vieille 1 , Kevin L. Epting 2 , Robert M. Kelly 2 and J. Gregory Zeikus 1 1 Department of Biochemistry ... example, thermostable xylose isomerases (XIs) (EC 5.3.1.5), which catalyze the isomerization of D -xylose to D-xylulose in vivo, are used for the...
Ngày tải lên : 22/02/2014, 07:20
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf

Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf

... The phosphoryl and pyridoxyl moieties of the P-pyridoxyl group are shown in red and magenta, respectively. The fractional solvent exposed area of the P-pyridoxyl group is 0.39 and 0.082 for the ... P-pyridoxyl group to the solvent. The fluorescence decay of pyridoxamine and of the P-pyridoxyl-labeled protein were monitored at 393 nm upon excitation at 326 nm....
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf

Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf

... A ˚ ke Engstro ¨ m at the Peptide Synthesis and Analysis Laboratory of the Department of Medical Biochemistry and Microbio- logy, Uppsala University. The SP6 primer 5¢-ATT TAG GTG ACA CTA TAG-3¢ and the three ... was thus similar to that of the porcine liver enzyme. The specificity of the recombinant enzyme towards phosphohistidine was supported by the finding th...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx

Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx

... production of cystatin F compared to the ubiquitous inhibitor, cystatin C, we measured the cystatin contents in cell lysates and culture media of cells grown for 2 and 4 days with cystatin F and cystatin ... of the cystatin F gene and promoter. The structures of the human cystatin F (CST7)andcystatinC(CST3) genes are illustrated schematically at the top. In the...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana docx

Tài liệu Báo cáo Y học: Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana docx

... a nonglycosylated triplet at position Asn70 [48]. The majority of the peroxidases carry one or two putative glycans. Seven appear to be nonglycosylated. Therefore, the high number of glycans found ... [64] have studied the promoter activity and wound-induction of HRPC and -E types, and of AtPCa and AtPEa. Most remarkably, C2, and only C2, responded strongly to wo...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: A novel, inducible, citral lyase purified from spores of Penicillium digitatum docx

Tài liệu Báo cáo Y học: A novel, inducible, citral lyase purified from spores of Penicillium digitatum docx

... as the standard. RESULTS Induction of citral lyase Initially, the reproducibility of the results was hindered by variations in the citral lyase activity of the P. digitatum spores. Remarkably, ... hydratase and aldolase activity in a single enzyme. No other enzyme has been reported to catalyse the conversion of citral into methylheptenone and acetaldehyde, or a simil...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin potx

Tài liệu Báo cáo Y học: Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin potx

... endothelial layer of the artery was removed by bubbling with oxygen for 10 s. Absence of the endothelial layer was confirmed by the lack of relaxation in response to a 30-min perfusion of acetylcholine ... This sensitivity is well within the capability of the ES-MS system employed. The primary structural diversity displayed within amphibian skin bradykinins may not simp...
Ngày tải lên : 21/02/2014, 15:20
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Tài liệu Báo cáo Y học: Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) pdf

Tài liệu Báo cáo Y học: Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) pdf

... localized in the cytoplasm (Fig. 6, middle panel). The GFP-tagged TRAF domains of TRAF2 and TRAF3 also localized to the cytoplasm whereas the TRAF domain of TRAF1 showed nuclear and cytoplasmic localization ... implicated mainly in signaling by members of the TNF receptor family, TRAF6 primarily transduces signals initiated by IL1/Toll receptors. In particular, TRAF4 has...
Ngày tải lên : 21/02/2014, 15:20
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Tài liệu Báo cáo Y học: Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis docx

Tài liệu Báo cáo Y học: Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis docx

... host naturally devoid of phosphatidylinositol (PtdIns). In the context of the bacterial membrane and in addition to de novo synthesis, the plant enzyme is capable of catalysing the exchange of the inositol ... Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis Anne-Marie Ju...
Ngày tải lên : 22/02/2014, 04:20
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx

Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx

... previously shown that at the beginning of the stationary phase the rate of synthesis of heat-shock proteins increases considerably, but only transiently [2,18]. Similarly, we observed that the steady-state ... the deletion of the UP12 encoding gene increased the sensitivity of the mutant to CCCP (Fig. 6), it did not have any detectable effects on the sensitivity...
Ngày tải lên : 22/02/2014, 07:20
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