Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf

Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf

Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf

... alignment of the interdomain and autolysis loops of mammalian chymotrypsins and trypsins. The enzyme that was used in this study, rat chymotrypsin B, is highlighted by bold type. The loops are boxed and ... 2). Finally it is of interest regarding the in vivo mechanism of inactivation, that chymotrypsin and trypsin, mixed in concentration ratios close to thos...
Ngày tải lên : 22/02/2014, 07:20
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Tài liệu Báo cáo Y học: Granule-bound starch synthase I A major enzyme involved in the biogenesis of B-crystallites in starch granules ppt

Tài liệu Báo cáo Y học: Granule-bound starch synthase I A major enzyme involved in the biogenesis of B-crystallites in starch granules ppt

... rate of amylopectin synthesis. The increase in crystallinity wit- nessed during these first 12 h is in line with the high rates observed for amylopectin synthesis. However between 12 and 58 h the ... amylose continues to accumulate at the final stage of starch synthesis in the absence of concomitant amylopectin synthesis (reviewed in [41]). In vitro synthesis of am...
Ngày tải lên : 22/02/2014, 07:20
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Tài liệu Báo cáo Y học: Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana docx

Tài liệu Báo cáo Y học: Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana docx

... present-day N-terminal domain. Conse- quently, the salt bridge may play an important role in the proper attachment of the N- and C-terminal domains during protein folding. Several other residues ... an invariantpartofallthreeclassesoftheplantperoxidase superfamily [44]. This motif (shown in grey shading in Fig. 1) includes Ser96 and Asp99 located at the beginning of heli...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: Structural and biochemical characterization of neuronal calretinin domain I– II (residues 1– 100) Comparison to homologous calbindin D28k domain I–II (residues 1 –93) pdf

Tài liệu Báo cáo Y học: Structural and biochemical characterization of neuronal calretinin domain I– II (residues 1– 100) Comparison to homologous calbindin D28k domain I–II (residues 1 –93) pdf

... (G66, R68 and G74) of Calb I–II lack the side-chain oxygens expected to provide calcium-binding ligands. The absence of calcium-binding to the EF-hand II motif of Calb has been shown in the individual ... for the full-length proteins. Keywords: calretinin; calcium; calbindin D 28k ; EF-hand; NMR secondary structure. Calretinin (CR) and calbindin D 28k (Calb) are homologo...
Ngày tải lên : 22/02/2014, 07:20
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Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx

Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx

... 2002 Extent of proteolysis as a function of temperature, time, and enzyme concentration BLG appeared to be virtually insensible to hydrolysis by either trypsin or chymotrypsin in the absence of a thermal unfolding ... of these hydrophobic residues are pointing inwards in the native protein structure, in which they line the hydrophobic cavity t hat characterizes all lip...
Ngày tải lên : 21/02/2014, 15:20
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Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt

Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt

... the activity of the enzyme was measured by the dinitrosalicylic acid method. Inhibition of the enzyme activity by glucose, maltose and cyclohexaamylose For the study of the enzyme inhibition by glucose, ... by the inhibitors of the enzymatic activity. Therefore the iodine staining method is well suited for extensive kinetic analyses of purified b-amylases. Stabili...
Ngày tải lên : 22/02/2014, 07:20
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Tài liệu Báo cáo Y học: Dissecting the effect of trifluoroethanol on ribonuclease A Subtle structural changes detected by nonspecific proteases ppt

Tài liệu Báo cáo Y học: Dissecting the effect of trifluoroethanol on ribonuclease A Subtle structural changes detected by nonspecific proteases ppt

... probably represents fragment 21–124, as in Fig. 5A. Quantification of the proteolytic susceptibility of RNase A. To gain further insight into the changes of the proteolytic susceptibility of RNase ... CD spectra in the far-UV region, on the other hand, indicate a detectable increase in the content of secondary structure only after the disruption of the native...
Ngày tải lên : 22/02/2014, 07:20
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... both O6 and N7 (Fig. 1C). The 2¢OH of the ribose is hydro- gen bonded to the main chain and the side chain of Asp142 (Fig. 1C). Both hydroxyl groups of the ribose are involved in coordinating a ... com- pared to the spectrophotometric methods. Because the enzymatic activity of PRTFDC1 towards hypoxanthine and guanine is only 0.26% and 0.09%, respectively, of...
Ngày tải lên : 15/02/2014, 01:20
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Tài liệu Báo cáo khoa học: Structural features of proinsulin C-peptide oligomeric and amyloid states pptx

Tài liệu Báo cáo khoa học: Structural features of proinsulin C-peptide oligomeric and amyloid states pptx

... structural role in the proper folding and disulfide bonding in insulin [2]. After proinsulin cleavage, it is released to the blood together with insu- lin in equimolar amounts. The 31-residue peptide ... distribu- tion of 100 l M proinsulin C-peptide in the presence of divalent Ca 2+ and Mg 2+ ions under native conditions (B), and of 100 l M C-peptide in the...
Ngày tải lên : 18/02/2014, 04:20
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Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of flavoproteins: electron transfer through the nitric oxide synthase flavoprotein domain pdf

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of flavoproteins: electron transfer through the nitric oxide synthase flavoprotein domain pdf

... in dual-flavin enzymes because of a number of factors, including the k 1 and k 2 of K eq A possibly being in uenced by changes in the FAD and FMN reduction state or by changes in NADP(H)-binding ... unclear at this point, but may certainly involve apparent differences in their CT and autoinhibitory insert elements, or elsewhere in the enzyme. Changing the set poi...
Ngày tải lên : 18/02/2014, 11:20
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