Tài liệu Báo cáo Y học: The effects of low pH on the properties of protochlorophyllide oxidoreductase and the organization of prolamellar bodies of maize (Zea mays) pot

Tài liệu Báo cáo Y học: Differential effects of arachidonoyl triﬂuoromethyl ketone on arachidonic acid release and lipid mediator biosynthesis by human neutrophils pot

... (1973) The enzymicincorporation of arachidonic acid into ether-containing choline and ethanolamine phosphoglycerides by deacylation-acylationreactions. Biochim. Biophys. Acta 326, 26–33.51. Yamashita, ... activity of arachidonoyl-CoA synthetase. J. Lipid Res. 25, 288–293.45. Norman, S.J. & Poyser, N.L. (1998) Detection of acyl-CoA syn-thetase, acyl-CoA. lysophospholipid acyltransferase and phos-pholipase ... constitutes the bulk of the fatty acid at the sn-2position of 1-alkyl-2-acyl-GPC under resting conditions[70]. Upon cell activation, most of this AA is replaced byother fatty acids. Finally,...

Tài liệu Báo cáo Y học: The effects of ring-size analogs of the antimicrobial peptide gramicidin S on phospholipid bilayer model membranes and on the growth of Acholeplasma laidlawii B ppt

... analogs on the thermotropic phase behavior of LMVs composed of dimyristoylglycerophosphocholine (Myr2Gro-PCho), dimy-ristoylglycerophosphoethanolamine (Myr2Gro-PEtn) and dimyristoylglycerophosphoglycerol ... (Lb¢)tothe(Pb¢) phase and the main transition from the conversion of the Pb¢to the Laphase. For a more detailed discussion of the thermotropicphase behavior of Myr2Gro-PGro and other members ... conformational effects. However, the generally positivecorrelation between the effective hydrophobicity and amphiphilicity of the GS ring-size analogs and the magnitude of their perturbation...

Tài liệu Báo cáo Y học: The effects of low pH on the properties of protochlorophyllide oxidoreductase and the organization of prolamellar bodies of maize (Zea mays) pot

... 2002 The effects of low pH on the properties of protochlorophyllide oxidoreductase and the organization of prolamellar bodies of maize (Zea mays)Eva Selstam1, Jenny Schelin1, Tony Brain2 and ... explanation of the acceleration of the low- pH driven spectral changes seen for the photoconverted and nonphotoconverted enzymes on addition of the adenylnucleotides or adenosine (Fig. 12) is that these ... form of a phosphorylated (or possibly adenylated) ternary complex of POR, its substrate PChlide, and NADPH. Dephospho-rylation of the photoconverted form of this complex by anendogenous phosphatase,...

Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx

... interaction (Fig. 9B) [49]. The above discussion rationalizes the observed effects of the Y7 4W mutation in PfTIM on the stability of the dimeric structure and catalytic activity. Examination of the ... includ-ing the proximal residues of the TIM active site. The isomerization of dihydroxyacetone phosphate to glycer-aldehyde 3-phosphate involves a proton abstractionfrom the substrate by the catalytic ... spectroscopy.Protein concentrations were determined by the Bradfordmethod [53] using BSA as a standard.Enzyme activity The enzyme activity of TIM was determined by the conver-sion of glyceraldehyde 3-phosphate...

Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx

... exchange and poly(U)-directedpoly(Phe) synthesis The ability of the deletion mutant of EF-Ts to stimulate the activity of EF-Tu was tested in guanine nucleotide exchange and poly(U)-directed poly(Phe) ... that of the wild-type strain,depending on the growth conditions used. The mutantstrain sensed amino acid starvation and synthesized the nucleotides guanosine 5¢-diphosphate 3¢-diphosphate and guanosine ... and guanosine 5¢-triphosphate 3¢-diphosphate at a lowercell density than the wild-type strain. Deletion of the coiled-coil motif only partially reduced the ability of EF-Ts to stimulate the guanine...

Tài liệu Báo cáo Y học: The glucose-speciﬁc carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx

... formation of NADPH in the presence of Glc6P dehydrogenase. This background activity musttherefore be due to phosphorylation of Glc released byslow hydrolysis of 3b, and not to phosphorylation of ... uptake concomitant with phosphory-lation of their substrates. The immediate source of high-energy phosphate is the phosphoryl carrier protein HPrwhich in turn is phosphorylated by phosphoenolpyruvate ... G. (1986) The oxidation of primary trimethylsilyl ethers toaldehydes: a selective conversion of a primary hydroxy group intoan aldehyde group in the presence of a secondary hydroxy group.J....

Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is speciﬁcally induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf

... galacto-oligosaccharides.ACKNOWLEDGEMENTS The authors thank Simon Flitter for the identiﬁcation and isolation of the galA containing phage clones and Matthew Illsley for the analysis of a-L-1,5-arabinofuranosidase activity in the ... 2 h and 20 h to determine the substratespeciﬁcity of the enzyme. The degradation of the polymer and subsequent oligosaccharide formation was analyzed byHPAEC and MALDI-TOF MS. HPAEC analysis ... vivoin potato resulting in a 30% reduction of the galactosylcontent of the pectin fraction of the cell walls [17].In this paper we describe the cloning, characterization and expression analysis of...

Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf

... governed byone or even two residues, but is most likely accomplished by the concerted effects of a combination of key residues.Further analysis of these and other mutant SODs iscurrently underway.ACKNOWLEDGEMENTSWe ... either iron or manganese.Selectivity of the proteins for their metal cofactor has beendemonstrated in vivo [3] and although apoenzymes of eachtype of SOD may be reconstituted by the addition ... MnSOD. We conclude thereforethat the position of this glutamine residue contributes tometal selectivity and is responsible for some of the diﬀerentphysicochemical properties of these SODs, and in...

Tài liệu Báo cáo Y học: The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168 pdf

... characterization of specific enzymes thatare involved in the biosynthesis of these structures and maylead to the discovery of potential therapeutic targets. Inaddition, the correlation of carbohydrate ... established by NMR was observed in the FAB-MS and MALDI-MS (Fig. 8) of the methylated PS-2. The MALDI-MS spectra shows a series of A-type primaryglycosyl oxonium ions and secondary ions of definedcompositions ... regarding the sequences of sugar unitswas obtained by FAB-MS of the methylated core OSderivative. Figure 2 and Table 3 shows a series of A-typeprimary glycosyl oxonium ions, and secondary ions, of defined...

Tài liệu Báo cáo Y học: Soluble silk-like organic matrix in the nacreous layer of the bivalve Pinctada maxima A new insight in the biomineralization ﬁeld pptx

... composition was very similar to the WSM, and consequently the EDTA-IM. Hydroxyproline, hydroxy-lysine and phosphoserine were not detected in WSM, WIM,EDTA-SM and EDTA-IM. The comparison of the amino ... nucleation, inhibition of crystalgrowth and control of aragonite-calcite polymorphism [43].At present, only a few constituents of these organicmatrices have been identiﬁed. One of the reasons ... in the biomineralization process. Someimportant roles were ascribed to them as the control of nucleation, the growth and the inhibition of crystal forma-tion [8,73]. The other compounds of the...

Xem thêm