... WSM.Glycosaminoglycan analysisGlycosaminoglycans are highly negatively charged because of the presence of sulfate ester and/or carboxyl groups.Therefore, they interact with cations and are precipitated ... Sulfated and nonsulfated glycosamino-glycans (GAGs) from the supernatant were estimated by the Whiteman Alcian blue binding technique [28,29], usingchondroitin sulfate as standard. The assay was ... 23,175–179.45. Balmain, J., Hannoyer, B. & Lopez, E. (1999) Fourier transforminfrared spectroscopy (FTIR) and X-ray diffraction analyses of mineral and organic matrix during heating of mother of pearl(nacre)...