Tài liệu Báo cáo Y học: Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis docx
Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf
... APDyTTPEMY-NH2were obtained from Dr A˚keEngstro¨m at the Peptide Synthesis and Analysis Laboratory of the Department of Medical Biochemistry and Microbio-logy, Uppsala University. The ... months. The phosphate content of the phosphopeptide was analysed by the malachite greenmethod [19 ]. The peptide concentration was calculated by using the molar absorbancy 13 600 of pNA at 315 nm. The nature ... degree of dephosphory-lation was calculated from the peak areas of phosphorylated and dephosphorylated peptides. In a control experiment, the stoichiometry of the cleavage was confirmed by the occurrence...
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Tài liệu Báo cáo Y học: Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana docx
... TC105068 1 1 1 3AtPCa TC1 212 67 2 2AtP32 TC 117 759 1 12AtP 31 TC123560 1 1 2AtP14 TC 111 256 22AtP46 TC 112 253 1 1AtP30 – 11 AtP18 TC 116 977 11 AtP36 TC122998 1 1AtP25 TC 117 275 11 AtP28 TC105 819 ... TC 115 545 17 218 37AtP 11 TC109595 24 16 31 AtP4 TC103 612 1 1 4 6 5 9 1 27AtP19 TC 115 663 15 2 7 24AtP17 TC1 215 24 2 2 13 5 22AtP15 TC109700 8 19 18AtPRC TC109863 5 1 2 2 3 13 AtP12 TC1 216 70 2 11 ... 27 4 49 10 54 18 AtP16 TC103486 10 0 2 4 23 12 9AtPCb TC1 212 66 49 1 12 3 15 1 1 19 10 1AtPEa TC 115 448 41 1 17 7 66AtP3 TC 115 510 19 232 53AtP2 TC109534 9 6 6 1 3 13 38AtP9 TC 115 527 8 2 17 3 8...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... mMphenylmethanesulfonyl fluoride. Cells were thendisrupted by treatment with lysozyme (1 mgÆmL )1 )onicefor 10 min followed by a single rapid cycle of freeze and thaw at 4 °C. The entire cell lysate ... at the beginning of the stationary phase the rate of synthesis of heat-shock proteins increases considerably,but only transiently [2 ,18 ]. Similarly, we observed that the steady-state amount of ... geneexpression, the structure and composition of the cell wall,DNA organization, synthesis of storage compounds such asglycogen and polyphosphate, and other cellular processes [1, 2]. As a result of these...
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Tài liệu Báo cáo khoa học: Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea pdf
... oxidation of the heme groups of the enzyme by various compounds was tested to identify the physiologicalelectron acceptor of the enzyme. The enzyme was notoxidized by the heterodisulfide of coenzyme ... amounts of th e 16 -kDa c-type cytochrome. The Fig. 2. Genomic o rganization of the genes e ncoding the subunits of the Hme complex from A. fulgidus. ThegenenamesannotatedbyTIGRaregivenabove the ... enzyme molecule has a mass of 15 0 kDa,this corresponds to 19 – 21 mol acid-labile sulfurÆ(molenzyme) )1 and 16 –20 mol nonheme ironÆ(mol enzyme) )1 .Analysis of the amino-acid sequence of the...
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Tài liệu Báo cáo Y học: Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis docx
... Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis Anne-Marie Justin, Jean-Claude Kader and Sylvie ... hostnaturally devoid of phosphatidylinositol (PtdIns). In the context of the bacterial membrane and in addition to de novo synthesis, the plant enzyme is capable of catalysing the exchange of the inositol ... reaction followed by synthesis is puzzling or in anycase very difficult to analyse in terms of kinetics. From afunctional point of view, if a reaction reverse of synthesis followed by synthesis using...
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Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
... cleaved by the restriction enzymes Nde1 and Xho1 and inserted between the Nde1 and the Xho1 sites of the pET21b. The HCV NS5B-1b and the GBV-B NS5B werepurified following the same procedure as for the ... efficiently replicates the 3 41 nt of the 3¢-end of the HCV minus-strand RNA in vitro and thatdeletion of the 3¢ 45 nt greatly impaired RNA synthesis [17 ]. Oh et al. [18 ] showed that RNA synthesis from ... 25 °C and stopped by the addition of 10 % (v ⁄ v) TCA. The radioactivity incorporated into newly synthesized RNAwas then determined. To quantify and analyze the 32P-labe-led RNA, the synthesis...
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
... The phosphoryl and pyridoxyl moieties of the P-pyridoxyl group are shown in red and magenta, respectively. The fractional solventexposed area of the P-pyridoxyl group is 0.39 and 0.082 for the ... concentrations.Substrate or ligand s 1 (ns) f 1 s2(ns) f2v2– 5 .15 0.34 1. 21 0.66 1. 1ATP, Mn2+5.27 0.37 1. 17 0.63 1. 06PEP, Mg2+5.52 0.47 1. 10 0.53 1. 12PEP, Mn2+6 .10 0. 51 1 .14 0.49 1. 06ATP, oxalate, ... lifetime of 1. 83 ns, whilethat of P-pyridoxyl bound to the protein could only be fitted by two exponential decays of 5 .15 ns and 1. 21 ns, withfractional intensities of 0.34 and 0.66, respectively (Fig....
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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx
... regulated by naturalinhibitors belonging to the cystatin superfamily. Theseinhibitors are of three major types [1] . Those of type 1, cystatins A and B (also called stefins), are approximately 10 0 ... cells (1 · 10 6mL )1 ) were seeded and cultured in the presence of 1 lMATRA for 4 days or in the presence of 0 .13 lMTPA for 2 days. After incubation for 4 and 2 days, respectively, the cells ... processing of the antigen rather than the MHC part of the complex. Manoury et al. [11 ]studied the processing of tetanus toxin in disrupted lysosomes from an EBV-transformed B-cell line. By using...
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Tài liệu Báo cáo Y học: Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin potx
... 265 .1 334.2 5T6 630.3 520.3 4 T 6 315 .7 260.6 4P7 727.4 419 .2 3P 7364.2 210 .1 3F8 874.4 322.2 2 F 8 437.7 16 1.6 2R9 10 30.6 17 5 .1 1R 9 515 .8 88 .1 1(Val1,Thr3,Thr6)-bradykininV1 10 0 .1 10 21. 5 ... 10 )6M 1 · 10 )3M) exposures and the endotheliallayer of the artery was removed by bubbling with oxygenfor 10 s. Absence of the endothelial layer was confirmed by the lack of relaxation in response ... to either potenti-ation of the effects of endogenous bradykinin by inhibition of catabolic proteases (snakes and scorpions) or by localdelivery of supraphysiological quantities of bradykinins(wasps...
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Tài liệu Báo cáo Y học: Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) pdf
... transduction by members of the TNFreceptor and IL1/Toll-receptor family [1, 2]. The TRAFproteins are characterized by a C-terminal homology domain of about 200 amino acids, called the TRAFdomain. The ... Henkler2,Ottmar Janssen3 and Harald Wajant 1 1Institute of Cell Biology and Immunology and 2Institute of Industrial Genetics, University of Stuttgart, Germany;3Institute of Immunology, Christian-Albrechts-University ... receptor-associated factor 1 gene(TRAF1) is up-regulated by cytokines of the TNF ligand family and modulates TNF-induced activation of NF-jBandc-JunN-terminal kinase. J. Biol. Chem. 274, 19 368 19 374.22....
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