Tài liệu Báo cáo Y học: Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin doc
... Short peptides are not reliable models of thermodynamic
and kinetic properties of the N-terminal metal binding site
in serum albumin
Magdalena Sokolowska
1
, Artur Krezel
1
, Marcin Dyba
1
, ... comparative study of thermodynamic and kinetic aspects
of Cu(II) and Ni(II) binding at the N-terminal b inding site of
human and bovine...
... transcription.
In summary, the data obtained in this study indicate the
conservation of the SRF DNA -binding and dimerization
domains during evolution and the divergence of the
transactivation domain. These ... C-terminal regions did not acti-
vate CA promoter and strongly repressed the c-fos promoter,
independently of the origin of the N-terminal region...
... University
{sgwater,jurafsky,manning}@stanford.edu
Abstract
Many factors are thought to increase the
chances of misrecognizing a word in ASR,
including low frequency, nearby disfluencies,
short duration, and being at the start of a turn.
However, few of these ... initial repetitions.)
3.2.1 Disfluency features
Perhaps the most interesting result in Table 2 is
that the ef...
... amyloid from the
C110)131 peptide.
Role of the NH
2
-terminal sequence
The role of the NH
2
-terminal sequence of the 124 centered
peptides included in this study was analyzed by comparing
the ... determination of the peptides by FT-IR
spectroscopy in D
2
O. Spectrograms of solutions containing the same
peptides included in Fig. 4 were analyzed in D
2
O...
... the in uence on d ecorin binding of chemical modi-
fication of Lys and Hyl side chains of collagens and
peptides. Derivatizations that eliminate the positive charge
of Lys/Hyl eliminate the binding ... of collagens and their CNBr
peptides demonstrated that acetylation eliminates their
binding to decorin. Lys/Hyl side chains are therefore
present at, or very ne...
... residue and/ or by the binding contribution from
theGalNAcresidue.
Docking the glycopeptide to the antibody the sugar ring
has little contact to the protein surface. Only the N-acetyl
methyl group ... struc-
ture into the binding cavity of SM3 and the binding epitope
obtained from STD NMR is very good.
Comparison with the X-ray and previous NMR structures
As men...
... parabutoporin and opistoporin
The peptides were prepared by solid-phase synthesis by
Ansynth Service B.V. (the Netherlands) using the Fmoc/
tert-butyl-based methodology with Rink resin as the solid
support. ... same family, independent of the continent
and region where they live. In this study, the biological
activity of parabutoporin and opistoporin 1 is compared
wit...
... quantitatively induced
erythrocytes aggregations.
Proteolytic stability
The proteolytic stability of d endrimeric peptides to trypsin
and chymotrypsin was determined using peptide and
enzyme in ratio of ... respectively. In contrast, the hemolytic
activity of the dendrimeric R4 peptides increased only
2.3 -and3 .3-foldfromR4toD
2
R4 and D
4
R4 peptides,
respectivel...
... (b -site
APP cleaving enzyme) at the N-terminus of Ab [4,5] and by
an as yet unidentified c-secretase at the C-terminus of Ab
(reviewedin[6]).Ab is the main component of vascular
amyloid in Alzheimer’s ... a
wavelength of 490 nm. Total lactate dehydrogenase was
determined by lysing cells in 0.2% Triton X-100 in DMEM/
10% fetal bovine serum and measuring the total a...
... points to changes in the dynamical
properties of the local environment of the P-pyridoxyl
group due to changes of the protein conformation induced
by the binding of PEP or the ternary combination.
Quenching ... high
binding affinity of PEP in the presence of Mn
2+
[28]. The
high affinity of PEP in the presence of Mn
2+
suggests a
specific interaction...