... transfectionHEK293 cells were cultured in DMEM containing 10%(v ⁄ v) fetal bovine serum and supplements (2 mml-gluta-mine, 100 UÆmL)1penicillin, 100 lg ÆmL)1streptomycin), at37 °C in a humidified ... thatincludes the proline-rich tyrosine kinase, Pyk2, Src and Grb2 [4,5], as well as Ras GEFs of the Ras-GRP (cal-DAG-GEF) family, through binding of Ca2+⁄ calmo-dulin (CaM) and diacylglycerol ... immunoglobulin (upperpanel) and anti-ERK1 ⁄ 2 immunoglobulin (lower panel), to confirmequal loading.Fig. 5. Serotonin increases intracellular Ca2+concentration through 5-hydroxytryptamine7(b)(5-HT7(b))...