...
4-12-1 Nakanarusawa
Hitachi, Ibaraki, 316-8511, JAPAN
shinnou@lily, dse. ibaraki, ac. jp
Abstract
In this paper, we propose a practical
method to detect Japanese homophone
errors in Japanese ... Proceedings of EACL '99
Detection of Japanese Homophone Errors by a Decision List
Including a Written Word as a Default Evidence
Hiroyuki Shinnou...
... for cathepsin B as a candidate
beta-secretase of Alzheimer’s disease. Biol Chem 386,
931–940.
5 Cataldo AM & Nixon RA (1990) Enzymatically active
lysosomal proteases are associated with amyloid ... Fab fragments prepared by
papain degradation of 2A2 mAb were purified by
affinity chromatography on a Protein A Sepharose
(Pharmacia, Uppsala, Sweden). On SDS ⁄ PAGE, the
biologic...
... respectively. An advantage with
this approach was that even though ATP also inter-
fered with these measurements (the performance of the
assay was reliable only within a limited range of probe
concentrations), ... -ATC ATC TCC
ATC GAC TAC TCC CTG-3¢, the antisense primer
5¢-AAG AAT
TCT AGA TTA ATG GTG ATG ATG GTG
ATG ATG GTG TGG GGT CAG CGG TGC AGC AGG
GGG GGT-3¢ (XbaI sites underlined;...
... Valencia,
Spain). Ten nanograms of the total RNA extracted was
used. Gene-specific primers for iNOS (forward primer, 5¢-
AGACGGATAGGCAGAGATTGG-3¢, and reverse pri-
mer, 5¢-ACTGACACTTCGCACAAAGC-3¢) and ... from mouse heart
was used, and quantification of iNOS mRNA was performed by
real-time RT-PCR. The relative level was calculated as
the ratio of inflammatory mRNA expression to b-actin...
... phosphatidyl-
inositol 3¢-kinase stimulates DNA synthesis by ras- and
p70, S6 kinase-dependent pathways. Mol Cell Biol 17,
248–255.
51 Katayama M, Hirai S, Kamihagi K, Nakagawa K,
Yasumoto M & ... and
5¢-CAUGUACCGAACCAAGUAGGA-3¢; control siRNA
5¢-GUACCUGACUAGUCGCAGAAG-3¢ and 5¢-UCUG
CGACUAGUCAGGUACGG-3¢. The specificities of these
sequences were confirmed by blasting against the Gen-...
... cleavage of mutated htt and
the release of an N-terminal fragment bearing the
mutation that is capable of nuclear localization [6,7].
HttEx1-polyQ N-terminal fragments with repeats of
fewer than ... We also observed a decrease in the three
major 20S proteasome activities that was CAG repeat
expansion dependent. This may reflect the fact that
proteasome has difficulty in degrading CAG...
... MINIREVIEW
Selection of stably folded proteins by phage-display with proteolysis
Yawen Bai and Hanqiao Feng
Laboratory of Biochemistry, National Cancer Institute, Bethesda, MD, USA
To facilitate the process of ... resistant to protease digestion than those
less stable and poorly folded; (b) M13 and fd phages are
resistant to cleavage by many proteases; (c) the surface g3p
proteins...
... concentration of AT and the
rates of interaction in the presence and absence of hep-
arin pentasaccharide (Table 1), one can calculate that
the half-life of enzyme activity in the absence of hep-
arin ... vascular disruption or the localized release
of heparin from the granules of mast cells which are
found lining the vasculature [28,29]. Thus the AT may
act as a sentinel to...
...
functional un-
certainty, as
defined by Kaplan and Zaenan [3]
and Kaplan and Maxwell [2]. In this paper, we
relate this characterization to that provided by
Tree ~,djoining Grammars (TAG), ... details are not relevant to the main point
of the paper. We note that, just as in a TAG, the
elementary trees which are the domains of depen-
dencies are available as a single...
... show that a sharp peak (quick up–down
conformational change of SERCA) was rarely observed
and that the lifetime of the elevated conformation was
apparently increased. The increased lifetime of the ... & Kodama A (1965) A new protein factor
promoting aggregation of tropomyosin. J Biochem 58,
107–108.
8 Yokokawa M, Wada C, Ando T, Sakai N, Yagi A,
Yoshimura SH & Takeyasu...