... ligand is involved in the initial homing of leuko-cytes to sites of in ammation [73,74]. The binding of PSGL-1 to P-selectin is largely due to two PTMs,namely an O-glycan that contains tetrasaccharidesialyl-LewisX, ... complete conversion in a matter of hours.As a demonstration of the biological relevance, thismethodology was used to model the P-selectin-bindingdomain of the mucin-like glycoprotein PSGL-1 [73,74].This ... (lacZ)reporter protein. In all cases, the proteins maintainednative function as well as being endowed withadditional lectin-binding properties. The two methods of modification, although employing different...