... ligands in the Mn-reconstituted enzyme and an additional water oxy-gen, which, together with the OD2 of Asp165, the NE2 of His79 and the NE2 of His169, formed anequatorial plane (Fig. 3B). The metal ... inhibi-tion of the binding of the superoxide substrate and the other to product inhibition by hydrogen peroxide.There are two mechanisms proposed for the reactioncycle of Mn ⁄ Fe-SOD: the 5-6-5 ... Mn-reconstitutedApeSOD, the metal ion was five-coordinate in trigonalbipyramidal geometry (Fig. 3A). Three of the ligands,OD2 of Asp165, NE2 of His79 and NE2 of His169,formed an equatorial plane. The other protein...