... ofVrp1p and Las17p in yeast and WIP and WASP-fam-ily proteins in mammalian cells are direct and resultin constitutive and apparently stable complexes[28,29,31,32]. In yeast, Vrp1p and Las17p ... yeast lysates and directly binds purified yeast and rabbit skeletalmuscle G-actin in vitro (Fig. 4B–D and data notshown). Mutations of K485 and R486 abolish bothtwo-hybrid interaction and binding ... of Singapore, Singapore4 Institute for Molecular Bioscience and ARC Special Research Centre for Functional and Applied Genomics, The University of Queensland,St Lucia, Australia5 UMR7156,...