Tài liệu Báo cáo Y học: Electrochemical, FT-IR and UV/VIS spectroscopic properties of the caa3 oxidase from T thermophilus docx
Tài liệu Báo cáo Y học: Electrochemical, FT-IR and UV/VIS spectroscopic properties of the caa3 oxidase from T. thermophilus docx
... anumber of enzymes, which show deviations to the centrallydiscussed oxidases. The study of these aberrant systems isimportant to understand the principles of these enzymes. The cytochrome c oxidase ... labelingexperiments.Interestingly potential titrations of the enzyme show aslightly different redox-dependent behavior. It may besuggested that the stronger cooperativity displays the modulation of the ... the Soret band was reported to absorb at 418 nm, the b-band at520 nm and the a–band at 550 nm [18]. The deviations of approximately 3 nm between the signals of horse heartcytochrome c and the...
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
... fluorescence intensity, Fois the fluorescence intensity at the start of the titration, F¥is the fluorescence intensity at saturating concentration of ligand, P the total protein concentration, and L ... due to minoralterations in the active site region that affect catalysisbut not substrate binding. On the other hand, the statisti-cal comparison between the structures of the labeled and unlabeled ... bindingTable 3. Rate constants for the quenching by acrylamide of singletexcited state of P-pyridoxyl bound to the protein in the presence of different ligands at saturating concentrations. The...
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Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf
... A˚keEngstro¨m at the Peptide Synthesis and Analysis Laboratory of the Department of Medical Biochemistry and Microbio-logy, Uppsala University. The SP6 primer 5¢-ATT TAGGTG ACA CTA TAG-3¢ and the ... environment of the N-phosphate of protein-bound phosphohistidine can influence its sensitivityto a phosphatase. Of potential interest in such a context was the finding that the rate of isomerization of ... obtained after a 1000-fold purification from the porcine liver cytosol (Table 1). At pH 5.6, the activity was only 9% of that at pH 7.5. No activity wasdetected at pH 9.0. The specific activity toward...
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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx
... The structures of the human cystatin F (CST7)andcystatinC(CST3) genes are illustratedschematically at the top. In the upstream region of the CST7 gene, the ATG (arrowhead) at position 0 is the ... interesting in this context. Although adetailed study of the cystatin F transport route was beyond the scope of the present investigation, the fact thatcystatin F is present in significant quantities ... colocalization forcystatin F. Analysis of the promoter region of the cystatin Fgene (CST7) showed that it, like that of the cystatin C gene(CST3), is devoid of typical TATA- and CAAT-box ele-ments....
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Tài liệu Báo cáo Y học: Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana docx
... Fig. 1) terminated the first part corres-ponding to the present-day N-terminal domain. Conse-quently, the salt bridge may play an important role in the proper attachment of the N- and C-terminal ... 3 and 4.Both methods support a constitutive expression of AtP1,AtP2, AtP9, AtP17, AtP4 and AtPCb. It is noteworthy thatthese AtPs represent highly diverse structures (Fig. 1,Table 2) and properties. ... indicate a lack of structure and susceptibility to proteolytic degradation.Experimental work on secreted peroxidases isolated from sweet potato tissue culture has demonstrated that theirN-termini...
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Tài liệu Báo cáo Y học: Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin potx
... activity of (Val1,Thr3,Thr6)-bradykinin in both of the smooth muscle preparationsemployed in this study, in contrast to the activity observedfor the Ala3,Thr6-substituted variant (Figs 7 and ... substitutedwith a prolyl residue. The propeptide convertases in thisspecies, appear to disregard the -Arg-Lys- doublet twopositions proximal to this substitution and rather cleave at the C-terminal ... secretion of the yellow-bellied toad, Bombina variegata.These peptides are the first amphibian skin bradykinins toexhibit amino acid substitutions at the Pro3 position of the bradykinin nonapeptide....
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Tài liệu Báo cáo Y học: Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) pdf
... differences in these cells in the distribution of TRAF4 between the cytoplasmic and the nuclear fraction (data not shown). This discrepancy cannotbe attributed to the GFP part of the various TRAF4 fusionproteins, ... Analysis of the various TRAF4 deletionmutants showed that the C-TRAF domain part of the TRAF domain is sufficient to direct the molecule into the sites of cell–cell contacts (Fig. 5).To verify ... and 2Institute of Industrial Genetics, University of Stuttgart, Germany;3Institute of Immunology, Christian-Albrechts-University of Kiel, GermanyTo gain insight in the subcellular localization of...
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Tài liệu Báo cáo Y học: Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin doc
... pointstoward slight differences in the conformation of the chelaterings. The characteris tic alternate pattern of the d–d bandsin the C D s pectra is dictated by the c onformation o f the ... conformation of the chelate system in the protein is alsointermediate between these two models. The Cu(II) stabilities at site II were measured directly, bytaking advantage from the presence of ... estimated spectrophotometrically at279 n m [6] and by Cu(II) titrations (see be low). Purities of both peptides were determined by potentiometric titrationsto exceed 98%.Peptide synthesisThe...
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Tài liệu Báo cáo Y học: Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis docx
... novo synthesis of PtdIns is most active. Thoroughenzymatic characterization of synthesis and exchange hasattemptedtounderstandifbothreactionsaremediatedby the same enzyme, but only one study has ... clear, for the first time, that a PtdIns synthase from Arabidopsis is able to catalyse both de novo synthesis of PtdIns and the exchange of the inositol moiety, but it is stilluncertain by what mechanism ... evaluation of net synthesis, one might wish to inhibit the exchange and one laboratory reported, to this end, that the addition of 5 mMEDTA was sufficient to abolish the exchange ability of PtdIns...
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Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc
... available to make this determination. In an attempt totest the simple hypothesis that class II XI stability at hightemperatures correlates with its N þ Q content, independent of the growth temperature ... activity and stability. Our results show that for class II XIs, N þ Qcontent relates to the enzyme’s functional temperature range and that BLXI thermostability is also directly related to the binding ... the binding of specific metals as cofactors. At the same time, the simple relationship between thermostability and N þ Qcontent may not hold in general, as it is not the case for the thermostable a-amylase...
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