Tài liệu Báo cáo Y học: Studies into factors contributing to substrate specificity of membrane-bound 3-ketoacyl-CoA synthases pot
Tài liệu Báo cáo Y học: Studies into factors contributing to substrate specificity of membrane-bound 3-ketoacyl-CoA synthases pot
... 2002 Studies into factors contributing to substrate specificity of membrane-bound 3-ketoacyl-CoA synthases Brenda J. Blacklock and Jan G. JaworskiDepartment of Chemistry and Biochemistry, Miami ... conver-sion of a hydroxylase to a desaturase [26].The objective of our work was to examine the substrate specificity of A. thaliana and B. napus FAE1 KCSs and to study the determinants of fatty acyl chain ... thischimeric enzyme changed the specificitytothat of the B. napus enzyme without loss of catalytic activity.Thus, this study was successful in identifying a domaininvolved in determining substrate specificity...
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Tài liệu Báo cáo khoa học: Ionic strength and magnesium affect the specificity of Escherichia coli and human 8-oxoguanine-DNA glycosylases pdf
... Series 4)and found only marginal differences for any of theenzyme substrate pairs [Fig. 5C shows an example of DNA glycosylase activity of OGG1 with the range of poly(ethylene glycol) 8000 and 0 ... Activity and specificity of Fpg and OGG1 in buffers with different concentrations of KCl and MgCl2. (A–C) Fpg, (D–F) glycosylaseactivity of OGG1, (G–I) AP lyase activity of OGG1. The extent of ... Activity and specificity of Fpg and OGG1 in buffers with different concentrations of KGlu and MgCl2in the presence of KPi. (A–C)Fpg, (D–F) glycosylase activity of OGG1, (G–I) AP lyase activity of...
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Tài liệu Báo cáo khóa học: Active-site residues and amino acid specificity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx
... 4¢-phosphopantothenoyl-CMP by cysteine is inhibited.PPC synthetase binds derivatives of cysteine To study the substrate specificity of His-CoaB,L-serine,L-alanine, cysteamine,L-cysteine methyl ester, ... covalently linked to an adenylyl group, whereas it iscovalently linked to a serine hydroxyl group in acyl carrierproteins. 4¢-Phosphopantetheine is also cofactor of enzymes that catalyze the biosynthesis ... (R)-4¢-phospho-N-pantothenoylcysteine synthetase. Theproposed intermediate of the (R)-4¢-phospho-N-panto-thenoylcysteine synthetase reaction, 4¢-phosphopantothe-noyl-CMP, was characterized by MALDI-TOF MS and...
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Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
... that the lower activity of the mutant may beascribed to the loss of an acceptor hydrogen bond to ametal-bound hydroxide, as a consequence of replacement of a carboxylate oxygen with an amide group.MATERIALS ... mutation of Asp153 by asparagine, the catalyticactivity of agmatinase (agmatine ureohydrolase, EC3.5.3.11) from Escherichia coli was reduced to about 5% of wild-type activity. Tryptophan emission ... (Asp153), may be reason-ably expected. This expectation is supported by our presentfindings of a markedly decreased activity of a D153Nvariant of E. coli agmatinase. From the enzymic properties of D153N...
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Tài liệu Báo cáo Y học: Studies on the nonmevalonate pathway of terpene biosynthesis The role of 2C-methyl-D -erythritol 2,4-cyclodiphosphate in plants docx
... 2C-methyl-D-erythritol4-phosphate can be converted into a cyclic diphosphate by theconsecutive action of 4-diphosphocytidyl-2C-methyl-D-erythritol synthase, 4-diphosphocytidyl-2C-methyl-D-erythritolkinase ... to the 2C-methyl-D-erythritol 2,4-cyclodiphosphateFig. 3.13C NMR signals of 2C-methyl-D-erythritol 2,4-cyclodiphosphate obtained by incubation of [U-13C5]2C-methyl-D-erythritol4-phosphate ... saturationcharacteristics.[1-3H]2C-methyl-D-erythritol 4-phosphate as well as[2-14C]2C-methyl-D-erythritol 2,4-cyclodiphosphate wereefficiently converted into lipid-soluble material. The amount of [1-3H]2C-methyl-D-erythritol...
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Tài liệu Báo cáo Y học: Electrochemical, FT-IR and UV/VIS spectroscopic properties of the caa3 oxidase from T. thermophilus docx
... terminal enzyme of therespiratory chain in mitochondria and many prokaryotes.As an integral membrane protein it catalyzes the reduction of dioxygen to water using electrons from cytochrome c.Four ... heme c. This potential differs for about 50 mV fromthe midpoint potential of isolated cytochrome c, indicatingthe possible shifts of the cytochrome c potential when bound to cytochrome c oxidase. ... labelingexperiments.Interestingly potential titrations of the enzyme show aslightly different redox-dependent behavior. It may besuggested that the stronger cooperativity displays themodulation of the enzyme to the...
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Tài liệu Báo cáo Y học: Interallelic recombination is probably responsible for the occurrence of a new as1-casein variant found in the goat species potx
... [18].Enzymatic hydrolysesTrypsin (Boehringer Mannheim) hydrolysis was carried outin 0.4% NH4HCO3,pH8.5,at37°C, for 4 h, in a substrate/ enzyme ratio of 5 0 : 1 (w/w). Dephosphorylationwith ... freeze-drying.Liquid chromatography/mass spectrometry analysis of proteins and peptidesThe whole caprine casein samples were fractionated by theprocedure of J aubert and M artin [21], modified by ... tryptic peptides on t he basis of the molecu larmass, taking into account the enzyme specificity and thereported amino-acid sequence of as1-Cas from differentspecies. Q uantitative a nalysis...
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Báo cáo khoa học: Structural basis for the changed substrate specificity of Drosophila melanogaster deoxyribonucleoside kinase mutant N64D docx
... deoxycytidine kinase; dGK, deoxyguanosine kinase; dN, deoxyribonucleosides; dT, deoxythymidine; dU, deoxyuridine; dC,deoxycytidine; dA, deoxyadenosine; dG, deoxyguanosine; hTK1, human thymidine ... sub-strate specificity and phosphorylates only thymidine(dT) and deoxyuridine (dU), whereas TK2 also phos-phorylates deoxycytidine (dC). dCK phosphorylatesdC, deoxyadenosine (dA) and deoxyguanosine ... 65–66 are hydrogen bonded to Tyr70, which forms a second hydrogen bond withthe 3¢-hydroxyl group of the substrate deoxyribose ring.In alignments of eukaryotic deoxyribonucleoside kin-ases,...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... agmatinenot only is practically not hydrolysed by wild-typearginase II, but it is also very poorly inhibitory to thisenzyme form. Thus, the altered specificity most prob-ably reflect an active ... activity of Asn149Asp on agmatine wasonly about 5% of the arginase activity of the wild-type enzyme. As measured by kcat⁄ Km, the catalyticefficiency of the Asn149Asp variant was found to beabout ... produced byagmatine hydrolysis, in studying the inhibitory effect of agmatine on arginine hydrolysis, reactions were followedby measuring the formation of ornithine, determined bythe method of Chinard...
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Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx
... The oxidation of primary trimethylsilyl ethers to aldehydes: a selective conversion of a primary hydroxy group into an aldehyde group in the presence of a secondary hydroxy group.J. Prakt. Chem. ... Methyl 2,3-anhydro-a-D-allopyr-anoside (2a) and methyl 3,4-anhydro-a-D-galactopyrano-side (2b) were obtained by desilylation of 1 mmol of the6-O-[dimethyl-(1,1,2-trimethylpropyl)silyl]-protected ... stereoselective synthesis of enantiomericallypure mono- and di-O-hexadecyl-b-D-glucosylglycerol ethers byepoxidation of an allyl b-D-glucopyranoside asymmetricallyinduced by the glucide moiety. Tetrahedron:...
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