Tài liệu Báo cáo Y học: Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin potx

Tài liệu Báo cáo Y học: Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin potx

Tài liệu Báo cáo Y học: Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin potx

... Novel bradykinins and their precursor cDNAs from European yellow-bellied toad ( Bombina variegata ) skin Tianbao Chen 1,2 , David F. Orr 1 , Anthony J. Bjourson 1 , Stephen ... Previously reported bradykinins from other Bombina species were not detected. Respective precursor cDNAs, designated BVK-1 and BVK-2, respect- ively, were cloned from a skin library b...
Ngày tải lên : 21/02/2014, 15:20
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Tài liệu Báo cáo Y học: Novel complexes of mammalian translation elongation factor eEF1AÆGDP with uncharged tRNA and aminoacyl-tRNA synthetase potx

Tài liệu Báo cáo Y học: Novel complexes of mammalian translation elongation factor eEF1AÆGDP with uncharged tRNA and aminoacyl-tRNA synthetase potx

... spectroscopy in peptide and protein analysis. In Encyclopedia of Analytical Chemistry: Instru- mentation and Applications (Meyers, R.A., ed.), pp. 5762–5779. John Wiley and Sons, New York. 23. ... eEF1Ba departs from eEF1A being ousted by newly synthesized aminoacyl-tRNA (E) [32]. The finding that the complex of eEF1A, eEF1Ba and nonhydrolyzable analog of GTP could be dissociated by...
Ngày tải lên : 21/02/2014, 15:20
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf

Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf

... phosphoryl and pyridoxyl moieties of the P-pyridoxyl group are shown in red and magenta, respectively. The fractional solvent exposed area of the P-pyridoxyl group is 0.39 and 0.082 for the open and ... 2002 conformational changes caused by the ligand are easily sensed by the P-pyridoxyl chromophore. Figure 4 shows the modeled structure of the P-pyridoxyl-PEP carboxykinase in the free...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf

Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf

... obtained from Promega, and LysC of Achromobacter lytii from Wako. The O-phosphorylated phosphopeptides EQTRsLDGR-NH 2 , DHTGFLtEY- VATRWC-NH 2 , DADEyL-NH 2 , EGDNDyIIPL-NH 2 , KKPRyEIRW-NH 2 , and ... GRRPsLFG and APDyTTPEMY-NH 2 were obtained from Dr A ˚ ke Engstro ¨ m at the Peptide Synthesis and Analysis Laboratory of the Department of Medical Biochemistry and Microbio- lo...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx

Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx

... exists in these two glycosylated forms, but also as one most likely unglycosylated species (lane 3, band C), with mobility exactly as recombinant cystatin F after deglycosylation with PNGase F ... inhibitor, cystatin C. Cystatin F purified from lysates of U937 cells showed three major forms car- rying two, one or no carbohydrate chains. Immunocyto- chemistry demonstrated a marked cytoplasmic...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana docx

Tài liệu Báo cáo Y học: Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana docx

... identity). Shinmyo et al. [64] have studied the promoter activity and wound-induction of HRPC and -E types, and of AtPCa and AtPEa. Most remarkably, C2, and only C2, responded strongly to wounding. ... flowers and siliques to represent whole healthy plants at all ages [12]. Whole plants were analyzed at day 3, 6, 15, 35 and 59 after sowing. Roots were analyzed at day 15, 35 and...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: Interaction of decorin with CNBr peptides from collagens I and II Evidence for multiple binding sites and essential lysyl residues in collagen pot

Tài liệu Báo cáo Y học: Interaction of decorin with CNBr peptides from collagens I and II Evidence for multiple binding sites and essential lysyl residues in collagen pot

... and activation by only two peptides from type II collagen (data not shown) and only by peptide a1(I) CB3 from t ype I collagen, as already known from the literature [49]. Peptide CB10 from type ... the charge. MATERIALS AND METHODS Materials Type I collagen from bovine skin and its CNBr peptides were already available and characterized by our laboratory [27–30]. Sulfosuc...
Ngày tải lên : 21/02/2014, 15:20
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Tài liệu Báo cáo Y học: Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) pdf

Tài liệu Báo cáo Y học: Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) pdf

... Henkler 2 , Ottmar Janssen 3 and Harald Wajant 1 1 Institute of Cell Biology and Immunology and 2 Institute of Industrial Genetics, University of Stuttgart, Germany; 3 Institute of Immunology, Christian-Albrechts-University ... exons 7–10. Purification and stimulation of primary human T-lymphocytes Mononuclear cells (PBMNC) were isolated from periph- eral blood of healthy donors by F...
Ngày tải lên : 21/02/2014, 15:20
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Tài liệu Báo cáo Y học: Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis docx

Tài liệu Báo cáo Y học: Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis docx

... phosphatidylinositol; PtdOH, phosphatidic acid; CDP-DAG, CDP-diacylglycerol; CMP, cytidine monophosphate; CTP, cytidine triphosphate. Enzymes: phosphatidylinositol synthase or CDP-diacylglycerol/ myo-inositol ... Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis Anne-Marie Justin, Jean-Claude Kader...
Ngày tải lên : 22/02/2014, 04:20
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Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc

Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc

... De Bruyne, C.K. (1988) D-xylose isomerase from Streptomyces violaceoniger: structural and catalytic roles of bivalent metal ions. Enzyme Microb. Technol. 10, 695–700. 41. van Bastelaere, P., Vangrysperre, ... finally dialyzed twice against 50 m M Mops (pH 7.0), this time without EDTA. The apoenzyme was divided into aliquots and stored at 2 70 8C until use. Enzyme assays BLXI activity was...
Ngày tải lên : 22/02/2014, 07:20
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