... Novel bradykinins and their precursor cDNAs from European
yellow-bellied toad (
Bombina variegata
) skin
Tianbao Chen
1,2
, David F. Orr
1
, Anthony J. Bjourson
1
, Stephen ... Previously reported bradykinins
from other Bombina species were not detected. Respective
precursor cDNAs, designated BVK-1 and BVK-2, respect-
ively, were cloned from a skin library b...
... spectroscopy in peptide and
protein analysis. In Encyclopedia of Analytical Chemistry: Instru-
mentation and Applications (Meyers, R.A., ed.), pp. 5762–5779.
John Wiley and Sons, New York.
23. ... eEF1Ba
departs from eEF1A being ousted by newly synthesized
aminoacyl-tRNA (E) [32]. The finding that the complex of
eEF1A, eEF1Ba and nonhydrolyzable analog of GTP
could be dissociated by...
... phosphoryl and pyridoxyl moieties
of the P-pyridoxyl group are shown in red and
magenta, respectively. The fractional solvent
exposed area of the P-pyridoxyl group is 0.39
and 0.082 for the open and ... 2002
conformational changes caused by the ligand are easily
sensed by the P-pyridoxyl chromophore. Figure 4 shows the
modeled structure of the P-pyridoxyl-PEP carboxykinase in
the free...
... obtained from Promega, and LysC of
Achromobacter lytii from Wako. The O-phosphorylated
phosphopeptides EQTRsLDGR-NH
2
, DHTGFLtEY-
VATRWC-NH
2
, DADEyL-NH
2
, EGDNDyIIPL-NH
2
,
KKPRyEIRW-NH
2
, and ... GRRPsLFG
and APDyTTPEMY-NH
2
were obtained from Dr A
˚
ke
Engstro
¨
m at the Peptide Synthesis and Analysis Laboratory
of the Department of Medical Biochemistry and Microbio-
lo...
... exists in these two glycosylated
forms, but also as one most likely unglycosylated species
(lane 3, band C), with mobility exactly as recombinant
cystatin F after deglycosylation with PNGase F ... inhibitor, cystatin C. Cystatin F purified
from lysates of U937 cells showed three major forms car-
rying two, one or no carbohydrate chains. Immunocyto-
chemistry demonstrated a marked cytoplasmic...
... identity). Shinmyo et al. [64] have studied the promoter
activity and wound-induction of HRPC and -E types, and
of AtPCa and AtPEa. Most remarkably, C2, and only C2,
responded strongly to wounding. ... flowers and siliques to represent whole healthy plants
at all ages [12]. Whole plants were analyzed at day 3, 6, 15, 35 and 59 after sowing. Roots were analyzed at day 15, 35 and...
... and activation by only
two peptides from type II collagen (data not shown) and
only by peptide a1(I) CB3 from t ype I collagen, as already
known from the literature [49].
Peptide CB10 from type ... the
charge.
MATERIALS AND METHODS
Materials
Type I collagen from bovine skin and its CNBr peptides
were already available and characterized by our laboratory
[27–30].
Sulfosuc...
... Henkler
2
,
Ottmar Janssen
3
and Harald Wajant
1
1
Institute of Cell Biology and Immunology and
2
Institute of Industrial Genetics, University of Stuttgart, Germany;
3
Institute of
Immunology, Christian-Albrechts-University ... exons 7–10.
Purification and stimulation of primary human
T-lymphocytes
Mononuclear cells (PBMNC) were isolated from periph-
eral blood of healthy donors by F...
... phosphatidylinositol; PtdOH, phosphatidic
acid; CDP-DAG, CDP-diacylglycerol; CMP, cytidine
monophosphate; CTP, cytidine triphosphate.
Enzymes: phosphatidylinositol synthase or CDP-diacylglycerol/
myo-inositol ... Phosphatidylinositol synthesis and exchange of the inositol head
are catalysed by the single phosphatidylinositol synthase 1
from
Arabidopsis
Anne-Marie Justin, Jean-Claude Kader...
... De
Bruyne, C.K. (1988) D-xylose isomerase from Streptomyces
violaceoniger: structural and catalytic roles of bivalent metal ions.
Enzyme Microb. Technol. 10, 695–700.
41. van Bastelaere, P., Vangrysperre, ... finally dialyzed
twice against 50 m
M Mops (pH 7.0), this time without
EDTA. The apoenzyme was divided into aliquots and stored
at 2 70 8C until use.
Enzyme assays
BLXI activity was...